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Cultivation of Bartonella henselae in an Ixodes ricinus cell line to assess this tick as a potential reservoir of the bacterium Cover

Cultivation of Bartonella henselae in an Ixodes ricinus cell line to assess this tick as a potential reservoir of the bacterium

Open Access
|Sep 2025

Figures & Tables

Fig. 1.

Experimental design schematic. d.p.i. – days post infection; B. henselae – Bartonella henselae; IRE/CTVM19 – Ixodes ricinus embryonic cell line
Experimental design schematic. d.p.i. – days post infection; B. henselae – Bartonella henselae; IRE/CTVM19 – Ixodes ricinus embryonic cell line

Fig. 2.

Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of monolayer cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection
Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of monolayer cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection

Fig. 3.

Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of supernatant cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection. * – statistically significant (P-value < 0.01)
Quantification of Bartonella henselae complementary DNA assessed by threshold cycle (Ct) values determined by real-time PCR in samples of supernatant cells from Ixodes ricinus embryonic cell line (IRE/CTVM19) cultures. Data were reported as the mean Ct ± standard error of the mean for three replicates of each day post infection. * – statistically significant (P-value < 0.01)
Language: English
Page range: 469 - 475
Submitted on: Jan 31, 2025
Accepted on: Aug 27, 2025
Published on: Sep 1, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2025 Violetta Zając, Jacek Sroka, Ewa Bilska-Zając, Angelina Wójcik-Fatla, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.