Antimicrobial agents used in the study and antimicrobial susceptibilities of Lactococcus garvieae
| Antimicrobial agent | MIC range (mg/L) | |||||
|---|---|---|---|---|---|---|
| Strain No. 5 | Strain No. 13 | Strain No. 64 | ||||
| MIC | Interpretation | MIC | Interpretation | MIC | Interpretation | |
| Benzylpenicillin | 0.5 | R | 1 | R | 0.5 | R |
| Clindamycin | ≤ 1 | R | ≤1 | R | ≤1 | R |
| Linezolid | ≤2 | S | ≤2 | S | ≤2 | S |
| Teicoplanin | ≤0.12 | S | ≤0.12 | S | ≤0.12 | S |
| Vancomycin | 0.5 | S | 0.5 | S | 0.5 | S |
| Tetracycline | 0.5 | S | ≤16 | R | 0.5 | S |
| Tigecycline | ≤0.06 | S | ≤0.06 | S | ≤0.06 | S |
| Moxifloxacin | 0.5 | S | 0.5 | S | 0.5 | S |
| Chloramphenicol | 4 | S | 2 | S | 4 | S |
| Rifampicin | ≤4 | R | ≤4 | R | ≤4 | R |
| Erythromycin | ≤0.12 | S | ≤0.12 | S | ≤0.12 | S |
| Trimethoprim-sulfamethoxazole | ≤0.12 | S | ≤10 | S | ≤10 | S |
Results of basic local alignment search tool analysis
| Bacterial strain No. | Query coverage (%) | Identity (%) | GenBank sequence length (base pairs) | Species | Accession No. |
|---|---|---|---|---|---|
| 5 | 95 | 98.74 | 1,471 | Lactococcus garvieae | LC377166.1 |
| 13 | 91 | 97.21 | 1,233 | Lactococcus garvieae | PP422542.1 |
| 64 | 88 | 85.16 | 895 | Lactococcus garvieae | MT000064.1 |
Groups of microorganisms identified in microbiological analyses
| Isolates | Number (n) | % of all milk samples | % of all strains |
|---|---|---|---|
| Micrococcus spp. | 2 | 1.69 | 4.88 |
| Enterococcus sp. | 1 | 0.85 | 2.44 |
| Streptococcus spp. | 10 | 8.47 | 24.39 |
| Lactococcus spp. | 3 | 2.54 | 7.32 |
| Corynebacterium sp. | 1 | 0.85 | 2.44 |
| Coagulase-negative staphylococci | 18 | 15.25 | 43.90 |
| Coagulase-positive staphylococci | 5 | 4.24 | 12.20 |
| Candida sp. | 1 | 0.85 | 2.44 |
| Contamination | 1 | 0.85 | – |
| No growth | 76 | 64.41 | – |
| Total | 118 | 100 | 100 |