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A preliminary study through lymphocyte immunophenotyping of the effects of different forms of phosphorus deficiency on dairy cattle blood haematological parameters and immune status Cover

A preliminary study through lymphocyte immunophenotyping of the effects of different forms of phosphorus deficiency on dairy cattle blood haematological parameters and immune status

Open Access
|Jun 2025

Figures & Tables

Fig. 1.

Gating strategy for immunophenotyping of bovine peripheral blood lymphocytes. A – single-cell gating; B – lymphocyte gating; C–F – fluorescence cytograms of positive cells
Gating strategy for immunophenotyping of bovine peripheral blood lymphocytes. A – single-cell gating; B – lymphocyte gating; C–F – fluorescence cytograms of positive cells

Morphology of blood samples of Friesian dairy cows with atypical or clinical hypophosphataemia

ParametersScope of standards (17)Group I (control)Group IIGroup IIIGroup IV
Pi (mmol/L)1.5–2.91.53 ± 0.10A,a1.12 ± 0.16A,b0.37 ± 0.05B,c0.33 ± 0.15B,c
WBC (×109/L)6.25–9.57.55 ± 1.20A,a5.63 ± 1.03B,b4.35 ± 1.45B,c3.50 ± 1.64C,d
GP (%)0–20.0 ± 0.00.0 ± 0.00.0 ± 0.00.0 ± 0.0
GS (%)23–3729.0 ± 7.07A,a23.0 ± 9.38A,b37.75 ± 11.09B,c54.25 ± 7.23C,d
L (%)53–6760.0 ± 4.24A,b68.75 ± 19.26a54.0 ± 15.51b41.75 ± 5.19c
TCD4+ (%) 24.64 ± 2.04a29.38 ± 3.48b29.44 ± 5.33b32.51 ± 4.27b
TCD8+ (%) 12.79 ± 0.57a7.91 ± 0.71b10.84 ± 2.66A,b11.61 ± 4.27A,b
TCD4/TCD8 1.94 ± 0.233.72 ± 0.452.77 ± 0.342.89 ± 0.57
MHC II (%) 39.29 ± 2.5a46.22 ± 3.83A,b50.64 ± 11.8b64.20 ± 5.33c
BCD21+ (%) 30.56 ± 5.6334.10 ± 3.8334.38 ± 5.0737.17 ± 9.75
RBC (×1012/L)5.0–7.07.03 ± 1.17a6.04 ± 0.59a4.95 ± 0.67b4.91 ± 0.77b
HGB (g/L)105–140101.5 ± 9.19A,a90.25 ± 10.72A,a63.75 ± 22.74bi'73.75 ± 11.41B,b
HCT (L/L)0.3–0.40.33 ± 0.04a0.28 ± 0.04A,b0.23 ± 0.03b0.23 ± 0.04b
MCV (fL)40–6046.5 ± 2.1247.25 ± 2.2245.75 ± 2.0647.25 ± 0.96
MCH (fmol/L)0.9–1.50.97 ± 0.030.89 ± 0.030.90 ± 0.030.93 ± 0.02
MCHC (mmol/L)16–2119.44 ± 0.4819.58 ± 0.2719.47 ± 0.3019.70 ± 0.48
PLT (×109/L)200–800330.5 ± 17.78647 ± 269.16469 ± 114.07388.5 ± 121.33
Language: English
Page range: 257 - 262
Submitted on: Dec 30, 2024
Accepted on: Jun 2, 2025
Published on: Jun 13, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2025 Beata Abramowicz, Łukasz Kurek, Urszula Lisiecka, Katarzyna Żarczyńska, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.