Fig. 1.
Fig. 2.
Reference sequence to partial F gene sequence match results after Newcastle virus RNA preservation as free virus and free RNA and by Flinders Technology Associates (FTA) card, RNASound and RNAstable
| Free virus | FTA cards | RNASound cards | Free RNA | RNAstable tubes | ||||||
|---|---|---|---|---|---|---|---|---|---|---|
| Sample number | Treatment (°C)/days | Sample number | Treatment (°C)/days | Sample number | Treatment (°C)/days | Sample number | Treatment (°C)/days | Sample number | Treatment (°C)/days | |
| 1 | –80/1 | 10 | –80/1 | 19 | –80/1 | 28 | –80/1 | 37 | –80/1 | |
| 7 | –80/35 | 11 | 25/1 | 20 | 25/1 | 29 | 25/1 | 38 | 25/1 | |
| 8 | 25/35 | 12 | 56/1 | 21 | 56/1 | 30 | 56/1 | 39 | 56/1 | |
| - | - | 14 | 25/28 | 22 | –80/28 | 31 | –80/28 | 40 | –80/28 | |
| - | - | 15 | 56/28 | 24 | 56/28 | 32 | 25/28 | 41 | 25/28 | |
| - | - | 16 | –80/35 | 26 | 25/35 | 34 | –80/35 | 42 | 56/28 | |
| - | - | 17 | 25/35 | 27 | 56/35 | 35 | 25/35 | 43 | –80/35 | |
| - | - | - | - | - | - | - | - | 44 | 25/35 | |
| - | - | - | - | - | - | - | - | 45 | 56/35 | |
| Matching samples/total samples | - | 3/9 | - | 7/9 | - | 7/9 | - | 7/9 | - | 9/9 |
Newcastle virus RNA integrity data for various preservation methods and controls at three temperatures across three time points_ The integrity of RNA was scored in a range from 1 to 10, where 10 was the highest integrity
| Temperature (°C) | FTA cards | RNASound cards | RNAstable tubes | Free virus | Free RNA | |
|---|---|---|---|---|---|---|
| –80 | 10 | N.D. | 9.1 | 9.4 | 8.7 | |
| Day 1 | 25 | 10 | N.D. | N.D. | 9.0 | 9.5 |
| 56 | 10 | N.D. | N.D. | 8.5 | 7.9 | |
| –80 | 10 | N.D. | 9.1 | 9.3 | 8.6 | |
| Day 14 | 25 | 10 | N.D. | N.D. | 8.9 | 9.5 |
| 56 | 10 | N.D. | N.D. | 8.3 | 7.4 | |
| –80 | 10 | N.D. | 9.2 | 8.6 | 8.6 | |
| Day 35 | 25 | 10 | N.D. | N.D. | 8.9 | 9.4 |
| 56 | 10 | N.D. | N.D. | 8.2 | 7.2 |