Have a personal or library account? Click to login
Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test Cover

Inhibitory effect of dimethyl sulfoxide on the development of gastrointestinal nematode larvae in the larval development test

Open Access
|Mar 2025

Figures & Tables

Fig. 1.

The observed percentage of unhatched eggs, larvae of the first and second stage (L1-L2) and larvae of the third stage (L3; percentage development) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Dots – arithmetic means; whiskers – 95% confidence intervals; asterisks (*) – statistically significantly lower percentages than in the control wells according to Dunnett’s test at α = 0.05
The observed percentage of unhatched eggs, larvae of the first and second stage (L1-L2) and larvae of the third stage (L3; percentage development) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Dots – arithmetic means; whiskers – 95% confidence intervals; asterisks (*) – statistically significantly lower percentages than in the control wells according to Dunnett’s test at α = 0.05

Fig. 2.

The five-parameter logistic curve (blue line) fitted to the observed percentage development of larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Red dots – exact values of tested concentrations indicated. Orange broken lines and arrows – DMSO concentrations inhibiting the development of 10%, 50% and 90% of L3
The five-parameter logistic curve (blue line) fitted to the observed percentage development of larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. at increasing dimethyl sulfoxide (DMSO) concentrations. Red dots – exact values of tested concentrations indicated. Orange broken lines and arrows – DMSO concentrations inhibiting the development of 10%, 50% and 90% of L3

Fig. 3.

The proportion of developed larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. (out of 100 counted L3) at increasing dimethyl sulfoxide (DMSO) concentrations. P-values of McNemar’s test
The proportion of developed larvae of the third stage (L3) of Haemonchus contortus, Trichostrongylus spp. and Oesophagostomum spp. (out of 100 counted L3) at increasing dimethyl sulfoxide (DMSO) concentrations. P-values of McNemar’s test

The observed percentage of eggs, larvae of the first and second stage (L1 and L2) and larvae of the third stage (L3; percentage development – PD) at increasing dimethyl sulfoxide (DMSO) concentrations

DMSO concentration Percentage of developmental stages observed after the 7-day incubation (%) a
nL3 (PD)Unhatched eggsL1 and L2
P-value b P-value b P-value b
0.0% (control)3095.5 ± 3.1 (87.5–100)-c0.05 ± 0.28 (0–1.54)-c4.43 ± 3.08 (0–12.5)-c
0.6%3097.0 ± 2.6 (89.2–100)0.8560.99 ± 1.60 (0–8.11)0.022*1.99 ± 2.26 (0–7.9)0.450
1.3%3092.8 ± 4.4 (81.0–100)0.3350.85 ± 1.27 (0–4.11)0.0686.35 ± 4.40 (0–19.0)0.683
2.6%3076.3 ± 8.8 (50.0–91.8)<0.001*0.49 ± 0.90 (0–3.13)0.56623.2 ± 8.8 (8.2–50.0)<0.001*
5.2%3027.6 ± 10.4 (10.8–50.0)<0.001*0.74 ± 1.43 (0–5.41)0.15771.7 ± 10.5 (50.0–89.2)<0.001*
10.4%305.7 ± 6.6 (0–33.7)<0.001*1.21 ± 1.53 (0–5.97)0.003*93.1 ± 6.6 (66.3–100)<0.001*
20.8%300.04 ± 0.22 (0–1.2)<0.001*0.84 ± 1.27 (0–4.62)0.07399.1 ± 1.3 (95.4–100)<0.001*
Language: English
Page range: 83 - 90
Submitted on: Sep 12, 2024
Accepted on: Mar 11, 2025
Published on: Mar 25, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year

© 2025 Marcin Mickiewicz, Zofia Nowek, Michał Czopowicz, Agata Moroz-Fik, Adrian-Valentin Potărniche, Kinga Biernacka, Olga Szaluś-Jordanow, Paweł Górski, Alistair Antonopoulos, Iwona Markowska-Daniel, Marián Várady, Jarosław Kaba, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.