Table 1.
Nutrient composition of diets in the Japanese Black calves
| Dietary component | Percentage of dry matter intake from commercial feed diet | |
|---|---|---|
| Milk replacer | Starter | |
| TDN | 116 | 75 |
| CP | 25 | 20 |
| Fat | 25 | 2 |
| NDF | 1 | 10 |
Table 2.
Primers used for real-time PCR expression analysis
| Gene | GenBank Accession No. | Product length (base pairs) | Primer | 5′–3′ sequence |
|---|---|---|---|---|
| IL-4 | NM_173921 | 117 | Forward | GCCCCAAAGAACACAACTGA |
| Reverse | GAGATTCCTGTCAAGTCCGC | |||
| IL-12p40 | NM_174356.1 | 101 | Forward | CACCCCGCATTCCTACTTCT |
| Reverse | TGACTTTGGCTGAGGTTTGG | |||
| IL-17A | NM_001008412.2 | 138 | Forward | ATCTCACAGCGAGCACAAGT |
| Reverse | GTGGGATGATGACTCCTGCC | |||
| IFN-γ | NM_174086 | 108 | Forward | TCAAATTCCGGTGGATGATCT |
| Reverse | CTTCTCTTCCGCTTTCTGAGG | |||
| TGF-β | NM_001166068.1 | 140 | Forward | TGACCCGCAGAGAGGAAATAG |
| Reverse | GTTCATGCCGTGAATGGTG | |||
| β-actin | NM_173979.3 | 76 | Forward | TCTTCCATAGGACACAATGCC |
| Reverse | GAGGCATACAGGGACAGCAC |
Fig. 1.
Changes in antibody titres against Pasteurella multocida, Mannheimia haemolytica and Histophilus somni. Solid circles – research group; hollow circles – control group. Results are expressed as the mean ± standard error (n = 24). O.D. – optimal density
Fig. 2.
Changes in numbers of peripheral lymphocyte, CD3+, CD4+, CD8+, CD14+ and MHC class-II cells. Solid circles – research group; hollow circles – control group. *–significant difference (P-value < 0.05) between two groups on the same day of life
Fig. 3.
Changes in relative gene expressions of interleukin (IL)-4, IL-12p40, IL-17A, interferon (IFN)-γ and transforming growth factor (TGF)-β. Solid circles – research group; hollow circles – control group. *–significant difference (P-value < 0.05) between two groups on the same day of life