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The pathological characteristics of enzootic nasal adenocarcinoma in goats Cover

The pathological characteristics of enzootic nasal adenocarcinoma in goats

Journal of Veterinary Research
Volume 69 (2025): Issue 1 (March 2025)
By:
Open Access
|Mar 2025

Figures & Tables

Fig. 1.

Dissection pictures of the nasal cavity in Chinese Anhui white goats with enzootic nasal adenocarcinoma. A – varying degrees of osteolysis (white arrows) in the frontal bone; B – tumour surface covered with a large amount of mucus; C – pale white and cauliflower-like tumours with a granular surface (white arrows); D – goat’s head incised along the paramedian sagittal plane exposing a large tumour (white arrow) deep in the nasal cavity obstructing the nasopharynx
Dissection pictures of the nasal cavity in Chinese Anhui white goats with enzootic nasal adenocarcinoma. A – varying degrees of osteolysis (white arrows) in the frontal bone; B – tumour surface covered with a large amount of mucus; C – pale white and cauliflower-like tumours with a granular surface (white arrows); D – goat’s head incised along the paramedian sagittal plane exposing a large tumour (white arrow) deep in the nasal cavity obstructing the nasopharynx

Fig. 2.

Representative haematoxylin-eosin and periodic acid–Schiff staining pictures of a caprine enzootic nasal adenocarcinoma. A – papillae located in the superficial layer of the tumour; B – acini located in the deeper layer of the tumour (broken black lines); C – papillae covered with a pseudostratified ciliated columnar epithelium; D – acinus consisting of a cuboidal epithelium of two to four layers (broken black line); E – remnant hyaline cartilage within the tumour tissue, with structure no longer intact (black arrows); F – epithelial-mesenchymal transition (black arrows) of tumour cells; G and H – mitotic figures (black arrow) of acinar and stromal tumour cells; I – a large number of PAS-positive granules on the cell surface; J – uneven, clumped purple granules aggregated in the acinar lumens (black arrows)
Representative haematoxylin-eosin and periodic acid–Schiff staining pictures of a caprine enzootic nasal adenocarcinoma. A – papillae located in the superficial layer of the tumour; B – acini located in the deeper layer of the tumour (broken black lines); C – papillae covered with a pseudostratified ciliated columnar epithelium; D – acinus consisting of a cuboidal epithelium of two to four layers (broken black line); E – remnant hyaline cartilage within the tumour tissue, with structure no longer intact (black arrows); F – epithelial-mesenchymal transition (black arrows) of tumour cells; G and H – mitotic figures (black arrow) of acinar and stromal tumour cells; I – a large number of PAS-positive granules on the cell surface; J – uneven, clumped purple granules aggregated in the acinar lumens (black arrows)

Fig. 3.

Representative immunohistochemical pictures of enzootic nasal adenocarcinomatous and healthy goat ethmoidal labyrinth. A and B – strong, diffuse cytoplasmic expression of pancytokeratin (Pan-CK) in a tumour epithelium; C – positive cytoplasmic expression of Pan-CK in a normal ethmoidal labyrinth epithelium; D and E – moderate, diffuse cytoplasmic expression of cytokeratin 7 (CK7) in a tumour epithelium; F – positive cytoplasmic expression of CK7 in a normal ethmoidal labyrinth epithelium; G and H – strong, diffuse cytoplasmic expression of cytokeratin 18 (CK18) in a tumour epithelium; I – positive cytoplasmic expression of CK18 in a normal ethmoidal labyrinth epithelium; J and K – no expression of olfactory marker protein (OMP) in a tumour epithelium; L – positive cytoplasmic expression of OMP in a normal ethmoidal labyrinth epithelium; M and N – Kiel 67-positive nuclei in a tumour epithelium with a Kiel 67 index of approximately 23%; O – near absence of Kiel 67-positive nuclei in a normal ethmoidal labyrinth epithelium with a Kiel 67 index of close to 0%; P, Q and R – negative control for tumour tissue and a normal ethmoidal labyrinth. Black boxes (A, D, G, J and P) indicate the regions of higher magnification in the right-adjacent images (B, E, H, K and Q)
Representative immunohistochemical pictures of enzootic nasal adenocarcinomatous and healthy goat ethmoidal labyrinth. A and B – strong, diffuse cytoplasmic expression of pancytokeratin (Pan-CK) in a tumour epithelium; C – positive cytoplasmic expression of Pan-CK in a normal ethmoidal labyrinth epithelium; D and E – moderate, diffuse cytoplasmic expression of cytokeratin 7 (CK7) in a tumour epithelium; F – positive cytoplasmic expression of CK7 in a normal ethmoidal labyrinth epithelium; G and H – strong, diffuse cytoplasmic expression of cytokeratin 18 (CK18) in a tumour epithelium; I – positive cytoplasmic expression of CK18 in a normal ethmoidal labyrinth epithelium; J and K – no expression of olfactory marker protein (OMP) in a tumour epithelium; L – positive cytoplasmic expression of OMP in a normal ethmoidal labyrinth epithelium; M and N – Kiel 67-positive nuclei in a tumour epithelium with a Kiel 67 index of approximately 23%; O – near absence of Kiel 67-positive nuclei in a normal ethmoidal labyrinth epithelium with a Kiel 67 index of close to 0%; P, Q and R – negative control for tumour tissue and a normal ethmoidal labyrinth. Black boxes (A, D, G, J and P) indicate the regions of higher magnification in the right-adjacent images (B, E, H, K and Q)

Fig. 4.

Ultrastructural manifestations of enzootic nasal adenocarcinoma. A – mature retrovirus-like particles in the intracytoplasmic vacuole and outside the cell (black arrows). Neighbouring tumour cells connected with tight junctions (white arrows). Scale bar = 2 μm. Corner view – details of the area indicated by the black box. Scale bar = 200 nm; B – immature intracytoplasmic particles (black arrowheads) and many secretory granules with heterogeneous density (white arrowheads) in the cytoplasm. Scale bar = 700 nm; C – sparse connections between stromal cells, and wide and obvious dilatation and vesiculation of the rough endoplasmic reticulum. Scale bar = 1 μm; D – small blood vessels in the stroma. Scale bar = 2 μm
Ultrastructural manifestations of enzootic nasal adenocarcinoma. A – mature retrovirus-like particles in the intracytoplasmic vacuole and outside the cell (black arrows). Neighbouring tumour cells connected with tight junctions (white arrows). Scale bar = 2 μm. Corner view – details of the area indicated by the black box. Scale bar = 200 nm; B – immature intracytoplasmic particles (black arrowheads) and many secretory granules with heterogeneous density (white arrowheads) in the cytoplasm. Scale bar = 700 nm; C – sparse connections between stromal cells, and wide and obvious dilatation and vesiculation of the rough endoplasmic reticulum. Scale bar = 1 μm; D – small blood vessels in the stroma. Scale bar = 2 μm
DOI: https://doi.org/10.2478/jvetres-2025-0010 | Journal eISSN: 2450-8608
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Language: English
Page range: 151 - 157
Submitted on: Aug 1, 2024
Accepted on: Feb 21, 2025
Published on: Mar 1, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
enzootic nasal adenocarcinoma,
enzootic nasal tumour virus,
goat,
immunohistochemistry,
ultrastructure
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2025 Lingxu Li, Zhen Wang, Weiling Qi, Yingjun Lv, Dawei Yao, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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