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The course of pigeon circovirus infection in young pigeons experimentally kept under conditions mimicking the One Loft Race rearing system Cover

The course of pigeon circovirus infection in young pigeons experimentally kept under conditions mimicking the One Loft Race rearing system

Journal of Veterinary Research
Volume 69 (2025): Issue 1 (March 2025)
By:
Open Access
|Mar 2025

Figures & Tables

Fig. 1.

Representative histograms of splenic B IgM+ flow cytometry analyses. A. Density plot of the extracellular staining for B IgM, Annexin V and 7-AAD. Three states of the cells were identified: viable cells, QIII (Annexin V/7-AAD = –/–); early apoptotic cells, QIV (Annexin/7-AAD = +/–); necrotic and late apoptotic cells, QI and QII (Annexin V/7-AAD = –/+ and +/+). B. Representative histogram of the sample with high apoptosis. C. Representative histogram of the sample with low apoptosis. The red color on the part B and C indicates on control cells (stained with anti-B IgM antibodies and 7-AAD), whereas the blue color indicates on apoptotic cells stained additionally with Annexin V
Representative histograms of splenic B IgM+ flow cytometry analyses. A. Density plot of the extracellular staining for B IgM, Annexin V and 7-AAD. Three states of the cells were identified: viable cells, QIII (Annexin V/7-AAD = –/–); early apoptotic cells, QIV (Annexin/7-AAD = +/–); necrotic and late apoptotic cells, QI and QII (Annexin V/7-AAD = –/+ and +/+). B. Representative histogram of the sample with high apoptosis. C. Representative histogram of the sample with low apoptosis. The red color on the part B and C indicates on control cells (stained with anti-B IgM antibodies and 7-AAD), whereas the blue color indicates on apoptotic cells stained additionally with Annexin V

The results of pigeon circovirus quantification by droplet digital PCR in blood throughout the experimental period

Day of sampling
071421283542
Mean PiCV viral load342.601,334.86,054.075,526.864,665.001,261.54202.69
SD of PiCV viral load859.243,964.5414,556.0811,115.7010,974.154,181.36679.48

The percentage of B IgM+ lymphocytes, including the apoptotic (APO) and necrotic (NECRO) cells, in the population of blood mononuclear cells of the experimental and control pigeons throughout the experiment

Day of samplingGroup
ExperimentalControl
B IgM+ (%)B IgM APO (%)B IgM NECRO (%)B IgM+ (%)B IgM APO (%)B IgM NECRO (%)
MnSDMnSDMnSDMnSDMnSDMnSD
07.730.91.99a0.640.450.787.170.833.32b2.10.160.15
77.601.282.461.350.220.337.170.833.322.10.160.15
147.140.983.501.490.28a0.226.951.263.690.780.67b0.48
217.390.671.680.440.120.087.040.371.560.580.140.08
287.630.821.070.320.080.076.920.701.180.020.020.03
356.930.672.100.560.160.176.360.341.670.390.310.16
427.700.742.010.630.04a0.057.270.553.102.700.17b0.17

The percentage of B IgM+ lymphocytes, including the apoptotic (APO) and necrotic (NECRO) cells, in the population of spleen mononuclear cells of the experimental and control pigeons on the last day of the experiment

ParameterGroup
ExperimentalControl
MnSDMnSD
B IgM+ (%)11.290.6011.231.02
B IgM APO (%)5.142.773.621.31
B IgM NECRO (%)0.120.090.110.05
DOI: https://doi.org/10.2478/jvetres-2025-0009 | Journal eISSN: 2450-8608
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Language: English
Page range: 1 - 6
Submitted on: Jul 8, 2024
Accepted on: Feb 14, 2025
Published on: Mar 1, 2025
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
apoptosis,
ddPCR,
One Loft Race,
pigeons,
pigeon circovirus
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2025 Daria Dziewulska, Bartłomiej Tykałowski, Ewa Łukaszuk, Tomasz Stenzel, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution 4.0 License.

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