The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenic Escherichia coli infection

Treatment	Group	Protein expression (arbitrary units)
Treatment	Group	5 LO	LTAH	LTCS
Control value	Control	0.12 ± 0.01^a	1.82 ± 0.24^a	0.73 ± 0.04^a
Control value	E. coli	0.76 ± 0.12^a^,***	1.71 ± 0.07^a	2.31 ± 0.36^a^,***
ACh	Control	0.65 ± 0.07^b	3.06 ± 0.11^b	2.15 ± 0.09^b
ACh	E. coli	1.63 ± 0.21^b^,***	2.81 ± 0.13^b	5.16 ± 0.42^b^,***
M2R antagonist	Control	0.09 ± 0.01^a	1.91 ± 0.16^a	0.62 ± 0.06^a
M2R antagonist	E. coli	0.83 ± 0.09^a^,***	1.23 ± 0.09^a^,**	3.29 ± 0.23^a^,***
M3R antagonist	Control	0.13 ± 0.05^a	1.87 ± 0.08^a	0.54 ± 0.07^a
M3R antagonist	E. coli	0.69 ± 0.14^a^,**	1.65 ± 0.11^a	2.86 ± 0.31^a^,***
α-7 nAChR antagonist	Control	0.11 ± 0.08^a	1.41 ± 0.05^a	0.63 ± 0.05^a
α-7 nAChR antagonist	E. coli	0.68 ± 0.15^a^,**	1.48 ± 0.07^a	1.99 ± 0.38^a^,**

1 Data are shown as the mean ± standard error of the mean.

1a, b – different superscript letters for any pair of values within a group for the same enzyme indicate significant difference (P-value < 0.05–0.001) between the control value, action of acetylcholine (ACh) and actions of particular antagonists;

1**, *** – significant difference between groups for the same enzyme and type of treatment (P-value < 0.01, P-value < 0.001)

Table 2.

Effects of acetylcholine (ACh) alone or antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) alone on leukotriene (LT)B4 and LTC4 secretion from the endometrium of gilts determined by ELISA

Treatment	Group	Concentrations (ng/g tissue)
Treatment	Group	LTB4	LTC4
Control value	Control	2.41 ± 0.14^a	0.85 ± 0.11^a
Control value	E. coli	3.37 ± 0.18^a^,**	1.29 ± 0.08^a^,*
ACh	Control	3.41 ± 017^b	1.45 ± 0.1^b
ACh	E. coli	4.25 ± 0.26^b^,*	1.82 ± 0.09^b
M2R antagonist	Control	2.42 ± 0.23^a	0.98 ± 0.09^a
M2R antagonist	E. coli	3.26 ± 0.22^a^,*	1.33 ± 0.11^a
M3R antagonist	Control	2.49 ± 0.15^a	0.81 ± 0.12^a
M3R antagonist	E. coli	3.09 ± 0.27^a	1.27 ± 0.15^a^,*
α-7 nAChR antagonist	Control	2.31 ± 0.17^a	0.92 ± 0.07^a
α-7 nAChR antagonist	E. coli	2.99 ± 0.26^a	1.16 ± 0.14^a

1 Data are shown as the mean ± standard error of the mean.

1a, b – different superscript letters for any pair of values within a group for the same leukotriene (LT) indicate significant difference (P-value < 0.05–0.001) between the control value, action of acetylcholine (ACh) and actions of particular antagonists;

1*, ** – significant difference between groups for the same LT and type of treatment (P-value < 0.05, P-value < 0.01)

ELISA results for the effects of acetylcholine (ACh) alone or with antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) on the leukotriene (LT)B4 (A) and LTC4 (B) secretion from the endometrium of gilts from the CON (grey bars, n = 5) and *E. coli* (black bars, n = 5) groups. a, b, c – different letters for any pair of values within a group indicate significant difference (P-value < 0.05–0.001) between the control values (CV), action of ACh and actions of particular antagonists with ACh; * P < 0.05, ** P < 0.01, *** P < 0.001 – statistical difference between groups for the same type treatment

Articles in this issue

DOI: https://doi.org/10.2478/jvetres-2024-0066 | Journal eISSN: 2450-8608 (formerly 2300-3235)

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Language: English

Page range: 571 - 581

Submitted on: Jun 21, 2024

Accepted on: Nov 22, 2024

Published on: Nov 29, 2024

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

endometritis,

acetylcholine,

B4 and C4 leukotriene production,

pig

Related subjects:

Life sciences,

Molecular biology,

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2024 Barbara Jana, Jarosław Całka, Michał Bulc, Dominika Kawka, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 68 (2024): Issue 4 (December 2024)

The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenic Escherichia coli infection

Figures & Tables

Fig. 1.

Table 1.

Table 2.

Effects of acetylcholine (ACh) alone or antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) alone on leukotriene (LT)B4 and LTC4 secretion from the endometrium of gilts determined by ELISA

Fig. 2.

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