The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenic Escherichia coli infection Cover

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The regulatory action of acetylcholine and its receptors on B4 and C4 leukotriene formation in the porcine endometrium after experimental inflammogenic Escherichia coli infection

Journal of Veterinary Research

Volume 68 (2024): Issue 4 (December 2024)

By: Barbara Jana , Jarosław Całka , Michał Bulc and Dominika Kawka

Open Access

|Nov 2024

Figures & Tables

Western blot results for the effects of acetylcholine (ACh) alone or with antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) on 5-lipoxygenase (5-LO; A), leukotriene (LT) A4 hydrolase (LTAH; B) and LTC4 synthase (LTCS; C) protein abundances in the endometrium of gilts from the control (grey bars, n = 5) and E. coli (black bars, n = 5) groups. Blots with representative bands for each enzyme in particular groups are depicted in Supplementary Figure 2. a, b, c – different letters for any pair of values within a group indicate significant difference (P-value < 0.05–0.001) between the control values (CV), action of ACh and actions of particular antagonists with ACh; *** – statistical difference between groups for the same type of treatment (P < 0.001)

ELISA results for the effects of acetylcholine (ACh) alone or with antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) on the leukotriene (LT)B4 (A) and LTC4 (B) secretion from the endometrium of gilts from the CON (grey bars, n = 5) and E. coli (black bars, n = 5) groups. a, b, c – different letters for any pair of values within a group indicate significant difference (P-value < 0.05–0.001) between the control values (CV), action of ACh and actions of particular antagonists with ACh; * P < 0.05, ** P < 0.01, *** P < 0.001 – statistical difference between groups for the same type treatment

Effects of acetylcholine (ACh) alone or antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) alone on leukotriene (LT)B4 and LTC4 secretion from the endometrium of gilts determined by ELISA

Treatment	Group	Concentrations (ng/g tissue)
Treatment	Group	LTB4	LTC4
Control value	Control	2.41 ± 0.14^a	0.85 ± 0.11^a
Control value	E. coli	3.37 ± 0.18^a^,**	1.29 ± 0.08^a^,*
ACh	Control	3.41 ± 017^b	1.45 ± 0.1^b
ACh	E. coli	4.25 ± 0.26^b^,*	1.82 ± 0.09^b
M2R antagonist	Control	2.42 ± 0.23^a	0.98 ± 0.09^a
M2R antagonist	E. coli	3.26 ± 0.22^a^,*	1.33 ± 0.11^a
M3R antagonist	Control	2.49 ± 0.15^a	0.81 ± 0.12^a
M3R antagonist	E. coli	3.09 ± 0.27^a	1.27 ± 0.15^a^,*
α-7 nAChR antagonist	Control	2.31 ± 0.17^a	0.92 ± 0.07^a
α-7 nAChR antagonist	E. coli	2.99 ± 0.26^a	1.16 ± 0.14^a

Western blot results for the effects of acetylcholine (ACh) alone or antagonists of muscarinic 2 and 3 receptor subtypes (M2R/M3R) and α-7 nicotinic ACh receptor (α-7 nAChR) alone on the 5-lipoxygenase (5-LO), leukotriene (LT)A4 hydrolase (LTAH) and LTC4 synthase (LTCS) protein abundances in unchallenged and E_ coli-challenged endometria of gilts

Treatment	Group	Protein expression (arbitrary units)
Treatment	Group	5 LO	LTAH	LTCS
Control value	Control	0.12 ± 0.01^a	1.82 ± 0.24^a	0.73 ± 0.04^a
Control value	E. coli	0.76 ± 0.12^a^,***	1.71 ± 0.07^a	2.31 ± 0.36^a^,***
ACh	Control	0.65 ± 0.07^b	3.06 ± 0.11^b	2.15 ± 0.09^b
ACh	E. coli	1.63 ± 0.21^b^,***	2.81 ± 0.13^b	5.16 ± 0.42^b^,***
M2R antagonist	Control	0.09 ± 0.01^a	1.91 ± 0.16^a	0.62 ± 0.06^a
M2R antagonist	E. coli	0.83 ± 0.09^a^,***	1.23 ± 0.09^a^,**	3.29 ± 0.23^a^,***
M3R antagonist	Control	0.13 ± 0.05^a	1.87 ± 0.08^a	0.54 ± 0.07^a
M3R antagonist	E. coli	0.69 ± 0.14^a^,**	1.65 ± 0.11^a	2.86 ± 0.31^a^,***
α-7 nAChR antagonist	Control	0.11 ± 0.08^a	1.41 ± 0.05^a	0.63 ± 0.05^a
α-7 nAChR antagonist	E. coli	0.68 ± 0.15^a^,**	1.48 ± 0.07^a	1.99 ± 0.38^a^,**

DOI: https://doi.org/10.2478/jvetres-2024-0066 | Journal eISSN: 2450-8608

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Language: English

Page range: 571 - 581

Submitted on: Jun 21, 2024

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Accepted on: Nov 22, 2024

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Published on: Nov 29, 2024

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

B4 and C4 leukotriene production,

Related subjects:

Molecular biology,

Microbiology and virology,

Life sciences, other,

Veterinary medicine

© 2024 Barbara Jana, Jarosław Całka, Michał Bulc, Dominika Kawka, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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