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The bioinformatics software used for the prediction and annotation of Mycoplasma gallisepticum strain MG-GD01/22 isolated from inoculated Chinese geese
| Database | Website |
|---|---|
| NR | HYPERLINK https://www.ncbi.nlm.nih.gov/refseq/about/nonredundantproteins/ |
| SwissProt | HYPERLINK http://www.ebi.ac.uk/swissprot/ |
| COG | HYPERLINK https://www.ncbi.nlm.nih.gov/research/cog-project/ |
| GO | HYPERLINK http://www.geneontology.org |
| KEGG | HYPERLINK http://www.kegg.jp/orhttp://www.genome.jp/kegg/ |
| VFDB | HYPERLINK http://www.mgc.ac.cn/VFs/ |
| CARD | HYPERLINK http://arpcard.mcmaster.ca |
| PHI-base | HYPERLINK http://www.phi-base.org/index.jsp |
| CAZy | HYPERLINK http://www.cazy.org/ |
PCR reaction system for amplification of Mycoplasma isolates from air-sac tissue of Chinese geese inoculated with Mycoplasma gallisepticum
| Component | Volume (μL) |
|---|---|
| TaKaRa Taq Version 2.0 plus dye | 5 |
| Forward primer (10μM) | 0.3 |
| Reverse primer (10μM) | 0.3 |
| ddH2O | 3.4 |
| Bacteria solution | 1 |
Primers used for PCR analysis of amplicons of Mycoplasma from air-sac tissue of Chinese geese inoculated with Mycoplasma gallisepticum
| Species | Primer sequences 5’→3’ | Fragment length (bp) | Annealing temperature (°C) |
|---|---|---|---|
| IGSR-F:GTAGGGCCGGTGATTGGAGTTA | |||
| Mycoplasma gallisepticum | IGSR-R:CCCGTAGCATTTCGCAGGTTTG | 792 | 57 |
Pathogen Host Interactions database analysis of the Mycoplasma gallisepticum strain MG-GD01 isolated from inoculated Chinese geese
| Taxon | Gene name | Gene number |
|---|---|---|
| Loss of pathogenicity | pmc1, cls | 10 |
| Increased virulence | algT, gyrA, glyA | 7 |
| Effector | pstB, groEL | 7 |
| Unaffected pathogenicity | FTT0673p/prsAp, secA2 | 3 |
| Reduced virulence | secA, Calcium-transporting | 2 |
Minimum inhibitory concentrations (MICs) of antimicrobials against a Mycoplasma gallisepticum reference strain (MG S6) and the isolate obtained in the experiment (MG-GD01)
| Drug name | MG S6 MIC | MG-GD01 MIC |
|---|---|---|
| Danofloxacin | 0.8 | 3.2 |
| Enrofloxacin | 0.4 | 3.2 |
| Tilmicosin | 0.2 | 0.4 |
| Tylosin | 0.2 | 0.4 |
| Acetylisovalery ltylosin tartrate | 0.1 | 0.3 |
| Tiamulin | 0.00625 | 0.0125 |
| Valnemulin | 0.00625 | 0.0125 |
| Spectinomycin | 0.4 | 3.2 |
The Comprehensive Antibiotic Resistance Database analysis of the Mycoplasma gallisepticum strain MG-GD01 isolated from inoculated Chinese geese
| Antibiotic type | Gene name | Gene number |
|---|---|---|
| Fluoroquinolone | parC, gyrB, parE, gyrA | 10 |
| Streptomycin | rpsL | 1 |
| Aminocoumarin | alaS, gyrB, parE, parY | 7 |
| Daptomycin | rpoB, rpoC | 3 |
| Rifamycin | rpoB | 2 |
| Other antibiotics | macB, dfrE | 2 |