Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
The list of antisera and reagents used in immunohistochemical investigations
| Primary antibodies | ||||
| Antigen | Code | Species | Working dilution | Supplier |
| GAL | T-5036 | Guinea pig | 1 : 2,000 | Peninsula Labs, San Carlos, CA, USA |
| nNOS | N218 | Mouse | 1 : 1,000 | Sigma-Aldrich, Saint Louis, MO, USA |
| NRG 1 | AA 198–229 | Rabbit | 1 : 1,000 | Antibodies-online, Aachen, Germany |
| PGP 9.5 | 7863-2004 | Mouse | 1 : 1,000 | BioRad, Hercules, CA, USA |
| VIP | 9535-0504 | Mouse | 1 : 1,000 | BioRad |
| Secondary antibodies | ||||
| Working dilution | Supplier | |||
| Alexa Fluor 488 donkey anti-rabbit IgG | 1 : 1,000 | ThermoFisher Scientific Waltham, MA, USA | ||
| Alexa Fluor 546 donkey anti-mouse IgG | 1 : 1,000 | ThermoFisher Scientific | ||
| Alexa Fluor 546 donkey anti-guinea pig IgG | 1 : 1,000 | ThermoFisher Scientific | ||
The degree of colocalisation of neuregulin 1 (NRG 1) and the neuronal isoform of nitric oxide synthase (nNOS) in the porcine small intestine
| Enteric plexus | Duodenum | Jejunum | Ileum |
|---|---|---|---|
| MP | 17.03 ± 1.35 %a | 31.04 ± 1.06 %b | 31.77 ± 1.14 %b |
| OSP | 26.25 ± 1.46 %a | 31.43 ± 1.22 %b | 29.52 ± 0.93 %b |
| ISP | 18.82 ± 1.28 %a | 15.96 ± 1.02 %a | 16.96 ± 1.19 %a |
The degree of colocalisation of neuregulin 1(NRG 1) and galanin (GAL) in the porcine small intestine
| Enteric plexus | Duodenum | Jejunum | Ileum |
|---|---|---|---|
| MP | 15.67 ± 0.94%a | 28.73 ± 0.81%b | 26.38 ± 1.37%b |
| OSP | 17.41 ± 1.00%a | 30.53 ± 1.29%b | 26.21 ± 0.84%c |
| ISP | 11.47 ± 1.21%a | 26.01 ± 1.10%b | 15.17 ± 1.24%a |
The percentage of neuregulin 1-positive neurons in the porcine small intestine
| Enteric plexus | Duodenum | Jejunum | Ileum |
|---|---|---|---|
| MP | 21.52 ± 0.98%a | 16.16 ± 0.88%b | 18.48 ± 0.66%b |
| OSP | 19.98 ± 0.69%a | 16.26 ± 0.84%b | 15.20 ± 0.63%b |
| ISP | 16.92 ± 1.19%a | 8.38 ± 0.55%b | 10.26 ± 0.70%b |
The degree of colocalisation of neuregulin 1 (NRG 1) and vasoactive intestinal polypeptide (VIP) in the porcine small intestine
| Enteric plexus | Duodenum | Jejunum | Ileum |
|---|---|---|---|
| MP | 32.39 ± 1.82%a | 25.50 ± 1.69%b | 29.17 ± 1.45%a |
| OSP | 41.91 ± 1.90%a | 24.96 ±2.24%b | 36.62 ± 1.43%c |
| ISP | 25.16 ± 0.94%a | 20.46 ± 1.23%b | 23.55 ± 0.87%a |