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Application of a liposomal subunit vaccine in chickens for reduction of Campylobacter gut colonisation Cover

Application of a liposomal subunit vaccine in chickens for reduction of Campylobacter gut colonisation

Journal of Veterinary Research
Volume 68 (2024): Issue 4 (December 2024)
By: Anna Łasica,  Renata Godlewska,  Jerzy Gubernator,  Anna Jakubiak-Augustyn,  Paweł Majewski and  Agnieszka Wyszyńska  
Open Access
|Nov 2024

Figures & Tables

Fig. 1.

A. RaptorX structure modelling for rCjaAEF-Tu (IA) and for rCjaCD (IIA). Modelling of the CjaA (IA) and CjaC (IIA) proteins with their native signal sequence is included for comparison. Protein epitopes (Ep) are shown in green (CjaA and CjaC) and black (EF-Tu and CjaD). Dashed arrows indicate elements not visible on selected orientation of protein molecule. B. The amino acid sequence of the rCjaAEF-Tu (IB) and rCjaCD (IIB) hybrid proteins. Lowercase letters denote the signal sequence at the amino terminus of the protein and the fragment at the carboxyl terminus covering the histidine tag. Epitopes of the CjaA (IB) and CjaC (IIB) proteins are indicated by underlining, epitopes of the EF-Tu (IB) and CjaD (IIB) proteins are marked in red, and insertion sites are highlighted in green
A. RaptorX structure modelling for rCjaAEF-Tu (IA) and for rCjaCD (IIA). Modelling of the CjaA (IA) and CjaC (IIA) proteins with their native signal sequence is included for comparison. Protein epitopes (Ep) are shown in green (CjaA and CjaC) and black (EF-Tu and CjaD). Dashed arrows indicate elements not visible on selected orientation of protein molecule. B. The amino acid sequence of the rCjaAEF-Tu (IB) and rCjaCD (IIB) hybrid proteins. Lowercase letters denote the signal sequence at the amino terminus of the protein and the fragment at the carboxyl terminus covering the histidine tag. Epitopes of the CjaA (IB) and CjaC (IIB) proteins are indicated by underlining, epitopes of the EF-Tu (IB) and CjaD (IIB) proteins are marked in red, and insertion sites are highlighted in green

Fig. 2.

Immunoreactivity and immunogenicity of hybrid rCjaAEF-Tu and rCjaCD proteins analysed by Western blot. A – Immunoreactivity of rCjaAEF-Tu with specific single antigen sera. Lane 1 – marker; lane 2 – purified rCjaAEF-Tu protein; B – Immunoreactivity of rCjaCD with specific single-antigen sera. Lane 1 – marker; lane 2 – purified rCjaCD protein; C – Immunogenicity of rCjaAEF-Tu detected with rabbit serum raised against whole hybrid protein. Lane 1 – marker; lane 2 – protein lysate from whole C. jejuni cells; D – Immunogenicity of rCjaCD detected with rabbit serum raised against whole hybrid protein. Lane 1 – marker; lane 2 – protein lysate from whole C. jejuni cells
Immunoreactivity and immunogenicity of hybrid rCjaAEF-Tu and rCjaCD proteins analysed by Western blot. A – Immunoreactivity of rCjaAEF-Tu with specific single antigen sera. Lane 1 – marker; lane 2 – purified rCjaAEF-Tu protein; B – Immunoreactivity of rCjaCD with specific single-antigen sera. Lane 1 – marker; lane 2 – purified rCjaCD protein; C – Immunogenicity of rCjaAEF-Tu detected with rabbit serum raised against whole hybrid protein. Lane 1 – marker; lane 2 – protein lysate from whole C. jejuni cells; D – Immunogenicity of rCjaCD detected with rabbit serum raised against whole hybrid protein. Lane 1 – marker; lane 2 – protein lysate from whole C. jejuni cells

Fig. 3.

Colonisation of chickens vaccinated with neutral or anionic liposomes containing hybrid proteins, rCjaCD and rCjaAEF-Tu, and of unvaccinated chickens challenged with strain 12/2 of Campylobacter jejuni. Viable C. jejuni cells recovered from the caeca of chickens were assessed on the specified days after the challenge. No significant differences (P-value < 0.05) were observed between groups. PBS – phosphate-buffered saline
Colonisation of chickens vaccinated with neutral or anionic liposomes containing hybrid proteins, rCjaCD and rCjaAEF-Tu, and of unvaccinated chickens challenged with strain 12/2 of Campylobacter jejuni. Viable C. jejuni cells recovered from the caeca of chickens were assessed on the specified days after the challenge. No significant differences (P-value < 0.05) were observed between groups. PBS – phosphate-buffered saline
DOI: https://doi.org/10.2478/jvetres-2024-0062 | Journal eISSN: 2450-8608
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Language: English
Page range: 487 - 496
Submitted on: Jun 2, 2024
Accepted on: Oct 28, 2024
Published on: Nov 6, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
vaccine,
liposomes,
hybrid proteins
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Anna Łasica, Renata Godlewska, Jerzy Gubernator, Anna Jakubiak-Augustyn, Paweł Majewski, Agnieszka Wyszyńska, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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