The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods

Brodzki, Piotr; Marczuk, Jan; Gorzkoś, Hubert; Lisiecka, Urszula; Szczubiał, Marek; Brodzki, Adam; Dąbrowski, Roman; Krakowski, Leszek; Głodkowska, Katarzyna; Brodzki, Nikodem

doi:10.2478/jvetres-2024-0057

Figures & Tables

The serum amyloid A level in the peripheral blood of cows at various periods of lactation DBD – days before drying; DBP – days before parturition; DPP – days postpartum. Experimental – cows with probiotic (n = 10); Control – cows without probiotic (n = 10); ** – statistical significance at – P-value ≤ 0.01 with respect to the control; a-c – statistical differences between the results for the material collected at different times in the group (P-value ≤ 0.01)

List of primary antibodies and secondary conjugates used in this study

Antibodies, dye	Cell type	Clone	Isotype
Mouse anti bovine CD4, PE	T helper cells	CC8	IgG2a
Mouse anti bovine CD8, FITC	T cytotoxic/suppressor cells	CC63	IgG2a
Mouse anti bovine CD11b, FITC	αM-integrin receptor subunit	CC126	IgG2b
Mouse anti-dog CD18, purified	β2-integrin receptor subunit	CA1.4E9	IgG1
Goat anti-mouse IgG (Fc), FITC	secondary antibody	polyclonal	polyclonal IgG
Mouse anti-bovine CD21, FITC	B lymphocytes	CC21	IgG1
Mouse anti-bovine CD25, RPE	Interleukin-2 receptor alpha chain	IL-A111	IgG1
Mouse anti-bovine Foxp3⁺, FITC	T regulatory cells	FJK-16s	IgG2a, kappa

Phenotyping of leukocytes in the peripheral blood of experimental (E) and control (C) cows during the experiment

Leukocyte percentage
Subpopulation	Group	7 DBD	14 DBP	7 DPP	21 DPP	60 DPP	90 DPP
CD18⁺	E	6.0 ± 0.6^a	5.1** ± 0.6^b	7.22** ± 2.9^a	4.6** ± 1.5^b	4.7** ± 1.2^b	4.2* ± 0.9^b
lymphocytes	C	6.6 ± 0.9^a	3.2 ± 0.6^b	2.8 ± 0.5^b	2.6 ± 0.8^b	2.8 ± 0.6^b	3.3 ± 0.3^b
CD18⁺	E	12.5 ± 3.9^a	11.7** ± 3.2^a	6.8* ± 1.6^b	11.0** ± 2.3^a	23.0** ± 1.6^c	24.7** ± 2.5^c
granulocytes	C	14.8 ± 3.0^a	6.2 ± 1.7^b	4.9 ± 1.7^b	4.4 ± 1.5^b	11.6 ± 2.6^c	18.3 ± 4.6^a
CD18⁺	E	11.7** ± 1.4^a	4.5 ± 1.5^b	8.6* ± 2.9^c	9.5** ± 1.8^c	18.0** ± 1.4^d	16.1* ± 1.8^d
monocytes	C	5.8 ± 1.4^a	5.2 ± 0.9^a	6.3 ± 0.6^a	4.6 ± 0.8^a	10.9 ± 1.4^b	13.4 ± 1.4^c
CD11b⁺	E	23.1* ± 4.4^a	23.7** ± 4.0^a	14.2 ± 1.2^b	15.5 ± 2.2^b	21.8** ± 2.1^a	30.4** ± 3.3^c
lymphocytes	C	19.5 ± 2.6^a	16.0 ± 2.1^b	13.2 ± 2.8^b	14.1 ± 2.0^b	17.0 ± 3.0^ab	25.2 ± 3.1^c
CD11b⁺	E	98.5* ± 0.9	98.9 ± 0.9	99.7 ± 0.1	99.7 ± 0.2	99.9 ± 0.0	99.6 ± 0.1
granulocytes	C	99.5 ± 0.3^a	98.1 ± 0.9^b	99.8 ± 0.1^a	99.7 ± 0.1^a	99.8 ± 0.1^a	99.9 ± 0.1^a
CD11b⁺	E	76.1 ± 4.2^a	78.1** ± 4.1^a	74.4 ± 5.8^a	61.8 ± 5.0^b	70.3** ± 4.2^ab	69.3 ± 4.1^ab
monocytes	C	73.3 ± 4.9^a	69.8 ± 7.1^a	71.2 ± 6.6^a	61.0 ± 8.9^b	56.0 ± 8.7^b	65.9 ± 4.1^b
CD21⁺	E	23.4 ± 3.78^a	21.14* ± 4.56^a	24.72 ± 3.86^a	24.74 ± 4.62^a	22.28** ± 4.38^a	33.32** ± 2.76^b
lymphocytes	C	24.88 ± 1.68	25.40 ± 3.02	25.62 ± 1.32	25.56 ± 3.32	25.50 ± 1.38	25.50 ± 1.64
CD25⁺	E	10.76 ± 2.28^a	8.13** ± 0.6^a	14.9** ± 2.19^b	20.4** ± 2.62^c	17.86** ± 3.0^bc	23.22** ± 2.94^c
lymphocytes	C	9.26 ± 1.28^a	6.51 ± 1.45^b	6.63 ± 1.66^b	10.75 ± 2.28^a	11.78 ± 1.18^a	15.8 ± 2.77^c
CD4⁺	E	28.96 ± 2.12^a	32.80 ± 2.66^b	34.04 ± 2.92^bc	35.72* ± 2.32^bc	36.62** ± 1.32^c	34.94** ± 1.66^bc
lymphocytes	C	26.64 ± 2.52^a	33.40 ± 1.72^b	33.72 ± 0.32^b	33.90 ± 0.52^b	32.62 ± 2.32^bc	30.72 ± 0.96^c
CD8⁺	E	10.56 ± 1.60	11.55* ± 1.12	11.96 ± 0.97	10.47* ± 1.69	10.15 ± 1.84	11.47** ± 1.48
lymphocytes	C	9.69 ± 1.11^a	13.18 ± 1.56^b	10.87 ± 0.78^a	13.04 ± 2.27^b	10.94 ± 2.12^a	16.56 ± 0.46^c
Foxp3⁺	E	42.54 ± 4.44^a	44.34 ± 3.06^a	49.44** ± 4.18^b	42.56 ± 4.88^a	47.68** ± 3.84^ab	46.22** ± 4.42^ab
lymphocytes	C	44.08 ± 3.52^ab	43.98 ± 1.44^ab	40.44 ± 4.18^a	41.10 ± 2.64^a	45.78 ± 3.26^b	33.92 ± 2.12^c

Composition of the total mixed ration and dry mass (DM) daily feed ration for lactating cows (kilograms/cow/day)

Feed component	Amount per cow, daily
Feed component	Kg	Kg DM
Maize silage	25.0	8.8
Haylage	8.0	3.2
Ensiled brewery spent grain	8.0	2.7
Wheat straw	0.8	0.7
Ensiled maize grain	2.5	1.7
Ground barley grain	1.5	1.3
Ground triticale grain	1.5	1.3
Ground rapeseeds	2.7	2.2
Extracted soyabean meal	2.0	1.7
Glycerine	0.3	0.24
Vitamin and mineral mixture	0.2	0.18
Sodium bicarbonate	0.2	0.2
Calcium carbonate	0.05	0.05
Total	52.75	24.27

The influence of probiotic administration on selected leukocyte subpopulations and the serum amyloid A concentration in the peripheral blood of dairy cows during different lactation periods

Figures & Tables

Fig. 1.

List of primary antibodies and secondary conjugates used in this study

Phenotyping of leukocytes in the peripheral blood of experimental (E) and control (C) cows during the experiment

Composition of the total mixed ration and dry mass (DM) daily feed ration for lactating cows (kilograms/cow/day)

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