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The effect of coffee husks used as pellet bedding material on the intestinal barrier, immune-related gene expression and microbiota composition in the broiler chicken caecum Cover

The effect of coffee husks used as pellet bedding material on the intestinal barrier, immune-related gene expression and microbiota composition in the broiler chicken caecum

Journal of Veterinary Research
Volume 68 (2024): Issue 3 (September 2024)
By: Jakub Biesek,  Aleksandra Dunisławska and  Wojciech Kozdruń  
Open Access
|Sep 2024

Figures & Tables

Fig. 1.

Relative analysis of expression of a panel of genes related to intestinal immune response and organism defence against pathogens in the caecal mucosa of broiler chickens bedded on litter with different proportions of coffee husks and wheat straw. CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)
Relative analysis of expression of a panel of genes related to intestinal immune response and organism defence against pathogens in the caecal mucosa of broiler chickens bedded on litter with different proportions of coffee husks and wheat straw. CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)

Fig. 2.

Relative abundance of indicator bacteria in caecal content of broiler chickens. C – broilers in the control group with litter of 100% wheat straw; CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)
Relative abundance of indicator bacteria in caecal content of broiler chickens. C – broilers in the control group with litter of 100% wheat straw; CH10 – broilers with litter of 10% coffee husks and 90% wheat straw; CH25 – broilers with litter of 25% coffee husks and 75% wheat straw; CH50 – broilers with litter of 50% coffee husks and 50% wheat straw; * – P ≤ 0.05 (n = 8/group)

Primer sequences used in the RT-qPCR reaction

GeneForward primerReverse primer
ACTBCACAGATCATGTTTGAGACCTTCATCACAATACCAGTGGTACG
G6PDHCGGGAACCAAATGCACTTCGTGGCTGCCGTAGAGGTATGGGA
CLDN1TCTTCATCATTGCAGGTCTGTCAACGGGTGTGAAAGGGTCAT
TJAP1AGGAAGCGATGAATCCCTGTTTCACTCAGATGCCAGATCCAA
MUC6TTCAACATTCAGTTCCGCCGTTGATGACACCGACACTCCT
AvBD1AAACCATTGTCAGCCCTGTGTTCCTAGAGCCTGGGAGGAT
CATHL2AGGAGAATGGGGTCATCAGGGGATCTTTCTCAGGAAGCGG
IL1BGGAGGTTTTTGAGCCCGTCTCGAAGATGTCGAAGGACTG
IL4GCTCTCAGTGCCGCTGATGGGAAACCTCTCCCTGGATGTC
IL6AGGACGAGATGTGCAAGAAGTTCTTGGGCAGGTTGAGGTTGTT
IL10CATGCTGCTGGGCCTGAACGTCTCCTTGATCTGCTTGATG
IL12BTTGCCGAAGAGCACCAGCCGCGGTGTGCTCCAGGTCTTGGG
IFNBACCAGATCCAGCATTACATCCACGCGTGCCTTGGTTTACG
IFNGACACTGACAAGTCAAAGCCGCAGTCGTTCATCGGGAGCTTG

Primer sequences for the identification of bacteria

BacteriaForward primerReverse primerReference
Universal bacteriaACTCCTACGGGAGGCAGCAGTGTATTACCGCGGCTGCTGGCAC(27)
Clostridium difficileTTGAGCGATTTACTTCGGTAAAGATGTACTGGCTCACCTTTGATATTCA(23)
Faecalibacterium prausnitziiACCATGAGAGCCGGGGGGGGTTACCTTGTTACGACTT(18)
Bifidobacterium spp.GCGTGCTTAACACATGCAAGTCCACCCGTTTCCAGGAGCTATT(23)
Lactobacillus spp.AGCAGTAGGGAATCTTCCACACCGCTACACATGGAG(13)
Escherichia coliCATGCCGCGTGTATGAAGAACGGGTAACGTCAATGAGCAAA(23)
Streptococcus spp.AGATGGACCTGCGTTGTGCTGCCTCCCGTAGGAGTCT(29)
DOI: https://doi.org/10.2478/jvetres-2024-0053 | Journal eISSN: 2450-8608
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Language: English
Page range: 443 - 449
Submitted on: Mar 28, 2024
Accepted on: Sep 9, 2024
Published on: Sep 23, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
coffee waste,
litter,
poultry,
caecum,
one health
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Jakub Biesek, Aleksandra Dunisławska, Wojciech Kozdruń, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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