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Biomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge Cover

Biomarkers of oxidative stress, biochemical changes, and the activity of lysosomal enzymes in the livers of rainbow trout (Oncorhynchus mykiss Walbaum) vaccinated against yersiniosis before a Yersinia ruckeri challenge

Journal of Veterinary Research
Volume 68 (2024): Issue 3 (September 2024)
By: Natalia Kurhaluk,  Joanna Grudniewska,  Agnieszka Pękala-Safińska,  Joanna Pajdak-Czaus,  Elżbieta Terech-Majewska,  Aleksandra Platt-Samoraj and  Halina Tkaczenko  
Open Access
|Sep 2024

Figures & Tables

Fig. 1.

Oxidative stress biomarkers estimated based on the levels of 2-thiobarbituric acid reactive substances (TBARS, A), aldehyde derivatives (B) and ketone derivatives (C) of oxidatively modified proteins, and total antioxidant capacity (D) in the liver tissue of rainbow trout orally immunised with Y. ruckeri vaccine and challenged with Y. ruckeri. Data are presented as means ± standard deviation (n = 10); * – significant differences (P-value < 0.05) between the untreated control group and the unvaccinated group challenged with Y. ruckeri; ** – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the vaccinated and unchallenged group; ## – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the unvaccinated group challenged with Y. ruckeri

Fig. 2.

Activity of the alanyl aminopeptidase (nmol·h-1·mg-1 protein), leucyl aminopeptidase (nmol·h-1·mg-1 protein), acid phosphatase (AcP, nmol·h-1·mg-1 protein), and β-N-acetylglucosaminidase (nmol·h-1·mg-1 protein) in the liver tissue of rainbow trout orally immunised against Y. ruckeri and challenged with this bacterium. Data are presented as means ± standard deviation (n = 10); * – significant differences (P-value < 0.05) between the untreated control group and the unvaccinated group challenged with Y. ruckeri; ** – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the vaccinated and unchallenged group; # – significant differences (P-value < 0.05) between the untreated control group and the vaccinated and unchallenged group; ## – significant differences (P-value < 0.05) between the vaccinated group subjected to the Y. ruckeri challenge and the unvaccinated group challenged with Y. ruckeri

Activity of superoxide dismutase (SOD, U·mg-1 protein), catalase (CAT, nmol·min-1·mg-1 protein), glutathione reductase (GR, nmol·min-1·mg-1 protein) and glutathione peroxidase (GPx, nmol·min-1·mg-1 protein) antioxidant enzymes in the liver tissue of rainbow trout orally vaccinated against Yersinia ruckeri and challenged with this bacterium

ParameterUntreated control groupVaccinated unchallenged groupUnvaccinated group challenged with Yersinia ruckeriVaccinated group challenged with Yersinia ruckeri
SOD, U·mg-1 protein643.90 ± 99.08*627.65 ± 38.09921.76 ± 139.25* ##656.44 ± 49.11##
CAT, nmol·min-1·mg-1 protein12.92 ± 0.67* #9.93 ± 0.59# **22.08 ± 1.88* ##11.52 ± 0.98** ##
GR, nmol·min-1·mg-1 protein15.19 ± 1.84* #25.13 ± 2.17#11.27 ± 3.70* ##24.19 ± 2.12##
GPx, nmol·min-1·mg-1 protein238.89 ± 23.26250.61 ± 43.82**186.96 ± 46.39##357.86 ± 70.71** ##

Activity of alanine aminotransferase (ALT, nmol·min-1·mg-1 protein) and aspartate aminotransferase (AST, nmol·min-1·mg-1 protein), AST/ALT ratio, lactate dehydrogenase activity (LDH, nmol·min-1·mg-1 protein), lactate and pyruvate levels (nmol·mg-1 protein) and the L/P ratio in the liver tissue of rainbow trout orally immunised against Yersinia ruckeri and challenged with this bacterium

ParameterGroup
Untreated control groupVaccinated unchallenged groupUnvaccinated group challenged with Yersinia ruckeriVaccinated group challenged with Yersinia ruckeri
ALT, nmol·min-1·mg-1 protein4.46 ± 0.48*4.25 ± 0.725.67 ± 0.65* ##4.21 ± 0.68##
AST, nmol·min-1·mg-1 protein3.36 ± 0.23*3.48 ± 0.344.12 ± 0.33* ##3.26 ± 0.33##
AST/ALT ratio0.75 ± 0.060.83 ± 0.110.76 ± 0.100.78 ± 0.09
LDH, nmol·min-1·mg-1 protein22.61 ± 0.8121.87 ± 0.2122.09 ± 0.4222.20 ± 0.33
Lactate, nmol·mg-1 protein63.50 ± 17.12*74.61 ± 19.64102.97 ± 18.09* ##64.94 ± 11.78##
Pyruvate, nmol·mg-1 protein29.56 ± 6.13*37.01 ± 5.9645.37 ± 11.38* ##33.31 ± 5.50##
L/P ratio2.22 ± 0.702.07 ± 0.642.39 ± 0.911.99 ± 0.49
DOI: https://doi.org/10.2478/jvetres-2024-0050 | Journal eISSN: 2450-8608
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Language: English
Page range: 325 - 336
Submitted on: Apr 6, 2024
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Accepted on: Sep 4, 2024
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Published on: Sep 23, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
challenge model,
rainbow trout,
oxidative stress,
antioxidant defences,
lysosomal enzymes
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Natalia Kurhaluk, Joanna Grudniewska, Agnieszka Pękala-Safińska, Joanna Pajdak-Czaus, Elżbieta Terech-Majewska, Aleksandra Platt-Samoraj, Halina Tkaczenko, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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