Table 1.
Phytochemical characterisation of methanolic extracts of Artemisia ludoviciana and Cordia boissieri
| Phytochemical group | A. ludoviciana | C. boissieri |
|---|---|---|
| Sterols and triterpenes | + | + |
| Sesquiterpene lactones | + | + |
| Coumarins | + | − |
| Saponins | + | + |
| Flavonoids | + | + |
| Alkaloids | − | − |
| Aromatics | + | + |
| Carbonyls | − | − |
| Phenolic oxides | + | + |
Table 2.
Inhibition activity of methanolic extracts of Artemisia ludoviciana and Cordia boissieri against Microsporum canis
| Extract | Concentration | % H |
|---|---|---|
| 1,250 μg/mL | 85.92 ± 7.34a | |
| A. ludoviciana | 2,500 μg/mL | 94.30 ± 5.84a |
| 5,000 μg/mL | 99.63 ± 1.24a | |
| 625 μg/mL | 77.11 ± 4.32b | |
| C. boissieri | 1,250 μg/mL | 94.12 ± 3.56a |
| 2,500 μg/mL | 100.00 ± 0.00a | |
| Imidazole | 1,000 μg/mL | 90.25 ± 3.44a |
Table 3.
Minimal inhibitory concentration (MIC) and minimal fungicidal concentration (MFC) of methanolic extracts of Artemisia ludoviciana and Cordia boissieri against Microsporum canis
| Extract | MIC | MFC |
|---|---|---|
| A. ludoviciana | 2,500 μg/mL | 5,000 μg/mL |
| C. boissieri | 1,250 μg/mL | 2,500 μg/mL |
Fig. 1.
Cell viability of HaCaT human keratinocyte cells exposed to different concentration of extracts of Artemisia ludoviciana and Cordia boissieri. Determination of viability was by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide assay (A) and trypan blue dye (B) Control – DMSO; P-value ≤ 0.05