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The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods Cover

The influence of probiotic administration on the phagocytic and oxidative burst activity of neutrophils and monocytes in the peripheral blood of dairy cows during different lactation periods

Journal of Veterinary Research
Volume 68 (2024): Issue 3 (September 2024)
By: Piotr Brodzki,  Hubert Gorzkoś,  Jan Marczuk,  Urszula Lisiecka,  Andrzej Junkuszew,  Leszek Krakowski,  Marek Szczubiał,  Nikodem Brodzki and  Katarzyna Głodkowska  
Open Access
|Aug 2024

Figures & Tables

Fig. 1.

Cytometric analysis of phagocytosis activity of neutrophils (A and B) and monocytes (C and D) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – percentage of neutrophils and monocytes that had engulfed bacteria; MFI – mean fluorescence intensities expressing individual cellular phagocytic activity (number of bacteria per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – significant differences between the results for the material collected at different times in the group (P-value < 0.01)
Cytometric analysis of phagocytosis activity of neutrophils (A and B) and monocytes (C and D) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – percentage of neutrophils and monocytes that had engulfed bacteria; MFI – mean fluorescence intensities expressing individual cellular phagocytic activity (number of bacteria per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – significant differences between the results for the material collected at different times in the group (P-value < 0.01)

Fig. 2.

Cytometric analysis of oxidative burst activity of neutrophils (A and B) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – the percentage of phagocytic cells that produced reactive oxygen substrates (measured by the conversion of DHR123 to R123); MFI – mean fluorescence intensities expressing enzymatic activity of phagocytic cells (measured by the amount of R123 per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – differences between the results for the material collected at different times in the group (P-value < 0.01)
Cytometric analysis of oxidative burst activity of neutrophils (A and B) in the peripheral blood of cows at various periods of lactation (mean ± standard error of the mean). DBD – day before drying; DBP – day before parturition; DPP – day postpartum; Experimental – cows provided with probiotic (n = 10); Control – cows without probiotic (n = 10); Phagocytic cells (%) – the percentage of phagocytic cells that produced reactive oxygen substrates (measured by the conversion of DHR123 to R123); MFI – mean fluorescence intensities expressing enzymatic activity of phagocytic cells (measured by the amount of R123 per cell). Statistical significance – * p ≤ 0.05; ** p ≤ 0.01 with respect to the control; a–c – differences between the results for the material collected at different times in the group (P-value < 0.01)

Composition of the total mixed ration (TMR) and dry mass (DM) daily feed ration for lactating cows

Dose componentAmount per cow, daily
kg TMRkg DM
Maize silage25.08.8
Haylage8.03.2
Ensiled brewery spent grain8.02.7
Wheat straw0.80.7
Ensiled maize grain2.51.7
Ground barley grain1.51.3
Ground triticale grain1.51.3
Ground rapeseed2.72.2
Extracted soyabean meal2.01.7
Glycerine0.30.24
Vitamin and mineral mixture0.20.18
Sodium bicarbonate0.20.2
Calcium carbonate0.050.05
Total52.7524.27
DOI: https://doi.org/10.2478/jvetres-2024-0043 | Journal eISSN: 2450-8608
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Language: English
Page range: 401 - 408
Submitted on: Jan 18, 2024
Accepted on: Jul 31, 2024
Published on: Aug 8, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
dairy cows,
neutrophils,
monocytes,
oxidative burst,
phagocytosis
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Piotr Brodzki, Hubert Gorzkoś, Jan Marczuk, Urszula Lisiecka, Andrzej Junkuszew, Leszek Krakowski, Marek Szczubiał, Nikodem Brodzki, Katarzyna Głodkowska, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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