Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses Cover

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Comparison of serological and molecular methods for differentiation between genotype A and genotype B strains of small ruminant lentiviruses

Journal of Veterinary Research

Volume 68 (2024): Issue 2 (June 2024)

By: Monika Olech and Jacek Kuźmak

Open Access

|Apr 2024

Figures & Tables

Inconclusive and indeterminate results obtained using the Eradikit SRLV Genotyping kit for classification of peripheral blood lymphocyte and serum samples of sheep and goats

Inconclusive OD						Indeterminate OD
No.	Subtypes	Host	Genotype A	Genotype B	Genotype E	Subtypes	Host	Genotype A	Genotype B	Genotype E
1.	A1	sheep	0.163	0.33	0.26	A1	goat	1.111	0.859	0.716
2.	A5	goat	0.251	0.202	0.172	A5	goat	0.589	0.777	0.634
3.	A5	goat	0.291	0.359	0.255	A5	goat	2.325	2.471	1.023
4.	A5	goat	0.323	0.262	0.21	A5	goat	1.806	1.726	0.265
5.	A5	goat	0.144	0.246	0.142	A12	goat	1.028	0.296	0.836
6.	A12	sheep	0.169	0.384	0.206	A12	goat	1.017	1.334	0.83
7.	A12	sheep	0.193	0.347	0.152	A12	goat	1.012	0.981	0.952
8.	A12	sheep	0.178	0.236	0.29	A12	goat	2.561	2.494	1.142
9.	A13	sheep	0.191	0.162	0.297	A13	sheep	1.256	1.026	1.159
10.	A17	goat	0.393	0.344	0.297	A13	sheep	0.775	0.82	0.701
11.	A17	goat	0.336	0.341	0.18	A13	sheep	0.151	0.547	0.687
12.	A18	sheep	0.349	0.325	0.374	A16	goat	0.944	0.763	0.314
13.	A18	sheep	0.206	0.255	0.355	A17	goat	2.559	2.433	2.797
14.	A18	sheep	0.395	0.209	0.348	A17	goat	2.456	2.438	0.434
15.	A23	sheep	0.183	0.262	0.298	A17	goat	1.181	1.378	0.736
16.	A23	sheep	0.17	0.216	0.241	A17	goat	0.912	0.921	0.344
17.	A27	goat	0.251	0.228	0.317	A17	goat	0.468	0.457	0.158
18.	A27	goat	0.211	0.168	0.119	A23	sheep	0.478	0.267	0.355
19.	B2	sheep	0.17	0.272	0.177	A27	goat	0.795	1.045	0.233
20.						B2	sheep	0.553	0.606	0.473

Nested real-time PCR and ELISA SRLV genotyping results for classification of peripheral blood lymphocyte and serum samples of sheep and goats

Test type	Samples (n)	Sample genotype (n)		Positive for genotype A (MVV) (n)		Positive for genotype B (CAEV) (n)		Positive for genotype E (n)		Inconclusive or indeterminate (n)
Test type	Samples (n)	A	B	Genotyped previously as A	Genotyped previously as B	Genotyped previously as A	Genotyped previously as B	Genotyped previously as A	Genotyped previously as B	Genotyped previously as A	Genotyped previously as B
Nested realtime PCR	86	73	—	62 (84.9%)	—	0	—	n/a	—	11 (15.1%)	—
Nested realtime PCR	86	—	13	—	0	—	7 (53.8%)	—	n/a	—	6 (46.2%)
Eradikit SRLV Genotyping ELISA	97	82	—	30 (36.6%)	—	14 (17.1%)	—	1 (1.2%)	—	37 (45.1%)	—
Eradikit SRLV Genotyping ELISA	97	—	15	—	2 (13.3%)	—	11 (73.3%)	—	0	—	2 (13.3%)

DOI: https://doi.org/10.2478/jvetres-2024-0025 | Journal eISSN: 2450-8608

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Language: English

Page range: 181 - 188

Submitted on: Nov 10, 2023

Accepted on: Apr 24, 2024

Published on: Apr 30, 2024

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

nested real-time PCR,

ELISA genotyping,

Related subjects:

Molecular biology,

Microbiology and virology,

Life sciences, other,

Veterinary medicine

© 2024 Monika Olech, Jacek Kuźmak, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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