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Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro Cover

Matrine and icariin can inhibit bovine viral diarrhoea virus replication by promoting type I interferon response in vitro

Journal of Veterinary Research
Volume 68 (2024): Issue 1 (March 2024)
By:
Open Access
|Mar 2024

Figures & Tables

Fig. 1.

Cytotoxic effects of matrine and icariin on Madin–Darby bovine kidney (MDBK) cells. A – Matrine’s effect on MDBK cell viability; B – Half-maximal inhibitory concentration (IC50) of matrine on MDBK cells; C – Icariin’s effect on MDBK cell viability; D – IC50 of icariin on MDBK cells. Data are presented as mean ± standard deviation of three independent experiments. DMSO – dimethyl sulphoxide solvent control; Control – cell maintenance solution control group; **** – P-value < 0.0001
Cytotoxic effects of matrine and icariin on Madin–Darby bovine kidney (MDBK) cells. A – Matrine’s effect on MDBK cell viability; B – Half-maximal inhibitory concentration (IC50) of matrine on MDBK cells; C – Icariin’s effect on MDBK cell viability; D – IC50 of icariin on MDBK cells. Data are presented as mean ± standard deviation of three independent experiments. DMSO – dimethyl sulphoxide solvent control; Control – cell maintenance solution control group; **** – P-value < 0.0001

Fig. 2.

Effects of the matrine and icariin against bovine viral diarrhoea (BVDV) infection in Madin–Darby bovine kidney (MDBK) cells. A – anti-BVDV effect of matrine in MDBK cells; B – Half-maximal effective antiviral concentration (EC50) of matrine on MDBK cells; C – anti-BVDV effect of icariin in MDBK cells; D – EC50 of icariin on MDBK cells; * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
Effects of the matrine and icariin against bovine viral diarrhoea (BVDV) infection in Madin–Darby bovine kidney (MDBK) cells. A – anti-BVDV effect of matrine in MDBK cells; B – Half-maximal effective antiviral concentration (EC50) of matrine on MDBK cells; C – anti-BVDV effect of icariin in MDBK cells; D – EC50 of icariin on MDBK cells; * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001

Fig. 3.

Evaluation of different administration methods of matrine and icariin on bovine viral diarrhoea virus (BVDV) infection in Madin–Darby bovine kidney (MDBK) cells. A – Effects of different administration methods of matrine on the BVDV 5′ untranslated region (UTR); B – Effects of different administration methods of icariin on the BVDV 5′UTR; C – Evaluation of prophylactic effects of matrine and icariin on the BVDV 5′UTR at different time points; **** – P-value < 0.0001; BVDV group – BVDV and MDBK; Virucide group – BVDV and extract incubated at 4°C for 1 h, MDBK added subsequently; Co-treatment group – BVDV, extract and MDBK incubated at 4°C for 1.5 h; Pre-treatment group – MDBK and extract incubated at 37°C for 3 h, challenged with BVDV subsequently; Post-treatment group – MDBK and BVDV incubated at 37°C for 1 h, extract added subsequently
Evaluation of different administration methods of matrine and icariin on bovine viral diarrhoea virus (BVDV) infection in Madin–Darby bovine kidney (MDBK) cells. A – Effects of different administration methods of matrine on the BVDV 5′ untranslated region (UTR); B – Effects of different administration methods of icariin on the BVDV 5′UTR; C – Evaluation of prophylactic effects of matrine and icariin on the BVDV 5′UTR at different time points; **** – P-value < 0.0001; BVDV group – BVDV and MDBK; Virucide group – BVDV and extract incubated at 4°C for 1 h, MDBK added subsequently; Co-treatment group – BVDV, extract and MDBK incubated at 4°C for 1.5 h; Pre-treatment group – MDBK and extract incubated at 37°C for 3 h, challenged with BVDV subsequently; Post-treatment group – MDBK and BVDV incubated at 37°C for 1 h, extract added subsequently

Fig. 4.

Effects of matrine and icariin on the expression levels of interferon alpha and beta (IFN-α/β) in Madin–Darby bovine kidney (MDBK) cells infected with bovine viral diarrhoea virus (BVDV). A – Effects of matrine and icariin on the secretion levels of IFN-β in MDBK cells; B – Effects of matrine on messenger RNA (mRNA) expression of IFN-α in MDBK cells; C – Effects of matrine on mRNA expression of IFN-β in MDBK cells; D – Effects of icariin on mRNA expression of IFN-α in MDBK cells; E – Effects of icariin on mRNA expression of IFN-β in MDBK cells; Control – negative group (without BVDV and drug); Matrine – group treated with matrine herb extract at 128 ng/mL; Icariin – group treated with icariin herb extract at 8 nM; + BVDV – group administered an extract and BVDV (100 TCID50); * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
Effects of matrine and icariin on the expression levels of interferon alpha and beta (IFN-α/β) in Madin–Darby bovine kidney (MDBK) cells infected with bovine viral diarrhoea virus (BVDV). A – Effects of matrine and icariin on the secretion levels of IFN-β in MDBK cells; B – Effects of matrine on messenger RNA (mRNA) expression of IFN-α in MDBK cells; C – Effects of matrine on mRNA expression of IFN-β in MDBK cells; D – Effects of icariin on mRNA expression of IFN-α in MDBK cells; E – Effects of icariin on mRNA expression of IFN-β in MDBK cells; Control – negative group (without BVDV and drug); Matrine – group treated with matrine herb extract at 128 ng/mL; Icariin – group treated with icariin herb extract at 8 nM; + BVDV – group administered an extract and BVDV (100 TCID50); * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001

Fig. 5.

Effects of matrine and icariin on the messenger RNA (mRNA) expression levels of innate immune response genes in MDBK cells at different time points. A – Effects of matrine on the expression levels of retinoic acid–inducible gene I (RIG-I); B – Effects of matrine on the expression levels of toll-like receptor 3 (TLR3); C – Effects of matrine on the expression levels of interferon regulatory factor 3 (IRF3); D – Effects of icariin on the expression levels of RIG-I; E – Effects of icariin on the expression levels of TLR3; F – Effects of icariin on the expression levels of IRF3; * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
Effects of matrine and icariin on the messenger RNA (mRNA) expression levels of innate immune response genes in MDBK cells at different time points. A – Effects of matrine on the expression levels of retinoic acid–inducible gene I (RIG-I); B – Effects of matrine on the expression levels of toll-like receptor 3 (TLR3); C – Effects of matrine on the expression levels of interferon regulatory factor 3 (IRF3); D – Effects of icariin on the expression levels of RIG-I; E – Effects of icariin on the expression levels of TLR3; F – Effects of icariin on the expression levels of IRF3; * – P-value < 0.05; ** – P-value < 0.01; *** – P-value < 0.001; **** – P-value < 0.0001
DOI: https://doi.org/10.2478/jvetres-2024-0013 | Journal eISSN: 2450-8608
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Language: English
Page range: 35 - 44
Submitted on: Aug 19, 2023
Accepted on: Mar 1, 2024
Published on: Mar 23, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
bovine viral diarrhoea virus,
matrine,
icariin,
antiviral effect,
immunoregulation
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Dongjie Cai, Zifan Shen, Bin Tian, Jie Chen, Yilin Zhang, Liuhong Shen, Ya Wang, Xiaoping Ma, Zhicai Zuo, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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