Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus

Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus

Journal of Veterinary Research

Volume 68 (2024): Issue 1 (March 2024)

By:

Maria Szczotka

and Jacek Kuźmak

Open Access

|Mar 2024

Figures & Tables

Cytokine expression of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Cytokine expression of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Cytokine expression in CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Cytokine expression in CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Cytokine expression in CD34+ stem cells isolated from the spleen of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Cytokine expression in CD34+ stem cells isolated from the spleen of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Cytokine expression in CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Cytokine expression in CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle in in vitro cell culture (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

The cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — The cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the blood of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Results of two-way analysis of variance of the cytokine levels in culture of CD34+ stem cells isolated from the bone marrow of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

The cytokine levels in culture of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — The cytokine levels in culture of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV−) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the spleens of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

The cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — The cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p40 – interleukin 12 subunit p40; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL)
IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma — Results of two-way analysis of variance of the cytokine levels in cultures of CD34+ stem cells isolated from the lymph nodes of bovine leukaemia virus–infected (BLV+) and uninfected (BLV-) cattle (pg/mL) IL-6 – interleukin 6; IL-10 – interleukin 10; IL-12p70 – interleukin 12 subunit p70; TNF-α – tumour necrosis factor alpha; IFN-γ – interferon gamma

The expression of bovine leukaemia virus glycoprotein 51 in foetal lamb kidney cells permanently infected with BLV

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ blood stem cells

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ bone marrow stem cells

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ spleen stem cells

The expression of bovine leukaemia virus glycoprotein 51 in CD34+ lymph node stem cells

Lack of fluorescence in the control cells

Mean values of cytokine secretion by bovine leukaemia virus–infected and uninfected CD34+ stem cells cultured in vitro

Sample type and presence of provirus (⁺/^-)		Mean secretion (pg/mL)
Sample type and presence of provirus (⁺/^-)		IL-6	IL-10	IL-12p40	IL-12p70	TNF-α	IFN-γ
Blood	BLV⁺	1.90	3.42	27.01	5.30	3.36	2.22
Blood	BLV^-	1.45	5.44	29.13	2.44	2.33	2.44
Bone marrow	BLV⁺	1.63	2.72	18.84	2.13	2.94	2.74
Bone marrow	BLV^-	1.89	2.70	17.12	1.13	2.47	2.26
Spleen	BLV⁺	0.33	1.87	53.03	1.46	3.82	1.67
Spleen	BLV^-	1.68	0.86	103.20	3.53	4.40	3.67
Lymph node	BLV⁺	1.21	1.36	49.81	2.19	3.72	1.93
Lymph node	BLV^-	0.78	0.71	40.52	2.09	3.89	1.90

DOI: https://doi.org/10.2478/jvetres-2024-0012 | Journal eISSN: 2450-8608

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Language: English

Page range: 19 - 33

Submitted on: Aug 29, 2023

Accepted on: Feb 28, 2024

Published on: Mar 23, 2024

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 times per year

Keywords:

bovine leukaemia virus (BLV),

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2024 Maria Szczotka, Jacek Kuźmak, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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Cytokine secretion in stem cells of cattle infected with bovine leukaemia virus

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Mean values of cytokine secretion by bovine leukaemia virus–infected and uninfected CD34+ stem cells cultured in vitro

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