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Molecular survey and genetic diversity of Plasmodium sp. infesting domestic poultry in northeastern Thailand Cover

Molecular survey and genetic diversity of Plasmodium sp. infesting domestic poultry in northeastern Thailand

Journal of Veterinary Research
Volume 68 (2024): Issue 1 (March 2024)
By:
Open Access
|Mar 2024

Figures & Tables

Fig. 1.

Map showing the area of sample collection in two provinces in the northeastern region of Thailand, consisting of Nongkhai (NK) and Maha Sarakham (MK)
Map showing the area of sample collection in two provinces in the northeastern region of Thailand, consisting of Nongkhai (NK) and Maha Sarakham (MK)

Fig. 2.

Plasmodium spp. infections in erythrocytes of backyard ducks, turkeys and geese (indicated by arrows). (A) Plasmodium sp.; (B) Plasmodium gallinaceum; (C) Plasmodium gallinaceum; (D) Plasmodium juxtanucleare. Parasite species were identified by DNA sequencing
Plasmodium spp. infections in erythrocytes of backyard ducks, turkeys and geese (indicated by arrows). (A) Plasmodium sp.; (B) Plasmodium gallinaceum; (C) Plasmodium gallinaceum; (D) Plasmodium juxtanucleare. Parasite species were identified by DNA sequencing

Fig. 3.

Phylogenetic tree based on partial cytochrome b gene sequences of Plasmodium in this study (indicated in bold typeface) together with 33 related sequences from different distributions in Thailand and neighbouring countries in the GenBank and MalAvi databases. Sequences were compared with the maximum likelihood method. The cytochrome b gene of two Haemoproteus sp. was used as the outgroup. The percentage of trees in which associated taxa clustered together is shown next to the branch
Phylogenetic tree based on partial cytochrome b gene sequences of Plasmodium in this study (indicated in bold typeface) together with 33 related sequences from different distributions in Thailand and neighbouring countries in the GenBank and MalAvi databases. Sequences were compared with the maximum likelihood method. The cytochrome b gene of two Haemoproteus sp. was used as the outgroup. The percentage of trees in which associated taxa clustered together is shown next to the branch

Lineages of 37 cytochrome b gene sequences from backyard poultry in Thailand with matches to MalAvi database and National Center for Biotechnology Information (NCBI) GenBank database sequences

Lineage namesParasitesSample IDsNCBI GenBank accession No.Closest sequences in NCBI GenBank (% similarity)
ACCBAD01Plasmodium sp.D3, D5, D11, D34, D38, D42, D43, D51, D53, T4, T6, T13, T15, T26, T34, T17OR341180MK390829.1 (100%), JN639001.1 (100%)
ORW1Plasmodium sp.D36, T11, T12, T14, T16, T18, T21, T22, T25, T27, T29, T31, T30, T32OR341179EF552403.1 (100%), KJ145050.1 (100%), KJ396632.1 (100%)
FANTAIL01Plasmodium sp.G5OR341184AY714196.1 (100%), HF543648.1 (100%)
ANAPLAPlasmodium sp.D1OR341178MK390829.1 (99.78%), JN639001.1 (99.78%)
GALLUS01P. gallinaceumT2, T7OR341181LC506179.1 (96.63-100%), LN835294.1 (96.63-100%)
MELGALP. gallinaceumT20, T28OR341183LC506179.1 (99.77%), LN835294.1 (99.77%)
GALLUS02P. juxtanucleareT9OR341182KU248845.1 (99.56%), KT290918.1 (99.56%)

Prevalence of Plasmodium spp_ infections in backyard poultry

Locations (Provinces)HostPrevalence of Plasmodium spp.Plasmodium species (n)
Common nameScientific nameMicroscopic examination (%)PCR examination (%)
NongkhaiDomestic duckAnas platyrhynchos domesticus1/30 (3.3)4/30 (13.3)Plasmodium sp. (4) Plasmodium sp. (2)
Domestic turkeyMeleagris gallopavo1/10 (10)5/10 (50)P. juxtanucleare (1)P. gallinaceum (2)
Maha SarakhamSwan gooseAnser cygnoides0/11 (0)1/11 (9.1)Plasmodium sp. (1)
Domestic duckAnas platyrhynchos domesticus2/30 (6.7)7/30 (23.3)Plasmodium sp. (7)
Domestic turkeyMeleagris gallopavo7/26 (26.9)20/26 (76.9)Plasmodium sp. (18)P. gallinaceum (2)
Swan gooseAnser cygnoides0/9 (0)0/9 (0)-
Total 11/116 (9.5)37/116 (31.9)

Association among Plasmodium spp_ infections with PCV levels

HostParasitesLevels of PCV (%) (mean ± SD)95% CI of average PCV value (%)P-valueNormal range (24)
Common nameScientific namePlasmodium spp. infections
DuckAnas platyrhynchos domesticusinfected (n = 11)34.91 ± 4.8731.64-38.180.6236-58%
uninfected (n = 49)33.83 ± 6.6831.89-35.77
TurkeyMeleagris gallopavoinfected (n = 25)38.92 ± 5.5036.59-41.240.0736-41%
uninfected (n = 11)35.09 ± 5.8231.18-39.00
GeeseAnser cygnoidesinfected (n = 1)47.00N/AN/A38-58%
uninfected (n = 19)41.10 ± 3.0338.93-43.27
DOI: https://doi.org/10.2478/jvetres-2024-0010 | Journal eISSN: 2450-8608
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Language: English
Page range: 101 - 108
Submitted on: Sep 6, 2023
Accepted on: Feb 19, 2024
Published on: Mar 23, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
avian,
haemosporidian pathogens,
spp.,
spp.,
spp.
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Wasupon Chatan, Khanchit Khemthong, Kittiya Akkharaphichet, Punwara Suwarach, Tossapol Seerintra, Supawadee Piratae, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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