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The first complete genome sequence and genetic evolution analysis of bovine norovirus in Xinjiang, China Cover

The first complete genome sequence and genetic evolution analysis of bovine norovirus in Xinjiang, China

Journal of Veterinary Research
Volume 68 (2024): Issue 1 (March 2024)
By:
Open Access
|Mar 2024

Figures & Tables

Fig. 1.

Phylogenetic tree of bovine norovirus. Black triangle – isolate obtained in this study; scale bar – nucleotide substitutions
Phylogenetic tree of bovine norovirus. Black triangle – isolate obtained in this study; scale bar – nucleotide substitutions

Fig. 2.

Amino-acid mutation of the VP1 protein of bovine norovirus blue – P2 subdomain; green – P1 subdomain; pink – S domain
Amino-acid mutation of the VP1 protein of bovine norovirus blue – P2 subdomain; green – P1 subdomain; pink – S domain

Fig. 3.

Bovine norovirus VP1 protein 3D model a – VP1 protein 3D models obtained in this study and superposition of the Bo/XJ-TC/01/CHN isolate VP1 protein on that of Bo/XJ-KS/02/CHN; b – superposition of the Bo/XJ-TC/01/CHN VP1 protein on that of MN122335, and of the Bo/XJ-KS/02/CHN VP1 protein on that of MZ573179; c – GIII.1 genotype VP1 protein, superposition of the Bo/XJ-TC/01/CHN VP1 protein on that of the GIII.1 genotype, and superposition of the Bo/XJ-KS/02/CHN VP1 protein on that of the GIII.1 genotype; mid-blue – P2 subdomain; mid-green – P1 subdomain; brown – S domain; black arrows – structural differences
Bovine norovirus VP1 protein 3D model a – VP1 protein 3D models obtained in this study and superposition of the Bo/XJ-TC/01/CHN isolate VP1 protein on that of Bo/XJ-KS/02/CHN; b – superposition of the Bo/XJ-TC/01/CHN VP1 protein on that of MN122335, and of the Bo/XJ-KS/02/CHN VP1 protein on that of MZ573179; c – GIII.1 genotype VP1 protein, superposition of the Bo/XJ-TC/01/CHN VP1 protein on that of the GIII.1 genotype, and superposition of the Bo/XJ-KS/02/CHN VP1 protein on that of the GIII.1 genotype; mid-blue – P2 subdomain; mid-green – P1 subdomain; brown – S domain; black arrows – structural differences

Sequence similarity comparison of reference sequences of bovine norovirus with the isolated Bo/XJ-KS/02CHN strain

N/AA (%)MN122335.1MK159169.1OK032546.2EU794907.1MN480761.1MZ573179.1NC029645.1AF097917.5AY126474.2
Complete genome93.06/98.1986.25/94.8385.84/90.5684.97/95.5093.46/98.1993.70/98.3786.6/95.94085.46/95.7685.33/95.57
ORF1P4893.81/96.9693.01/96.3594.02/97.2681.68/89.9794.22/97.2693.52/97.5784.40/93.3181.05/91.1982.14/91.19
NTPase93.74/99.1793.65/99.1794.20/99.4584.90/98.6294.57/10094.66/99.7286.00/98.6287.38/98.9085.82/99.17
p2291.85/97.8392.21/98.9192.39/97.8385.35/97.2589.41/94.5793.12/97.8386.31/96.7284.22/97.2586.28/97.25
VPg95.08/99.1894.54/98.3694.81/97.5483.88/94.2693.72/10092.90/97.5488.71/95.8785.21/95.0884.25/94.21
Pro94.27/99.4494.29/99.4593.92/10085.27/98.3497.24/10095.21/10086.74/97.7986.56/98.3485.82/98.34
RdRp94.66/99.4194.73/99.4193.74/98.6286.76/97.6394.66/99.4194.66/98.8189.53/98.8185.97/97.6386.17/97.43
ORF2VP191.39/96.7469.78/70.3668.39/70.3685.59/94.8392.10/97.5192.93/98.2885.91/94.2586.93/94.2586.11/94.83
ORF3VP291.69/96.7692.77/96.6266.62/63.4384.62/93.0691.54/96.7691.69/97.2284.92/92.1384.92/93.4685.56/92.13

Rate of infection with bovine norovirus among calves under three months old in Xinjiang, China

SeasonRegionTotal (n=974)
Spring(n=274)Summer(n=311)Autumn(n=166)Winter(n=226)Tacheng(n=15)Bortara(n=153)IlI(n=188)Kashga(n=424)Urumuq(n=44)Changji(n=150)
25 (9.12%)64 (20.58%)73 (43.98%)31 (13.72%)9 (60%)20 (13.07%)51 (27.13%)92 (21.70%)2 (4.55%)19 (12.67%)193 (19.82%)

Sequence similarity comparison of reference sequences of bovine norovirus with the isolated Bo/XJ-TC/01/CHN strain

N/AA (%)MN122335.1MK159169.1OK032546.2EU794907.1MN480761.1MZ573179.1NC029645.1AF097917.5AY126474.2
Complete genome93.48/96.7892.40/90.5786.60/90.6484.88/95.0692.81/97.3592.74/97.6086.36/95.4285.45/95.4184.97/95.38
ORF1P4895.03/96.6693.62/96.0595.04/96.9680.79/88.7593.41/96.9693.11/97.2683.79/93.3181.19/90.5881.26/90.58
NTPase93.55/98.3493.28/97.7995.30/98.6284.16/97.5193.74/98.6293.46/98.3485.36/97.2485.45/97.7984.44/98.07
p2291.85/97.2893.48/98.3794.20/97.2885.71/97.8089.05/94.5792.21/98.3784.31/96.1784.22/97.8085.90/96.70
VPg94.81/97.5494.26/97.5493.44/96.7282.51/93.4494.54/98.3691.53/95.9088.80/95.8783.84/94.2683.29/93.39
Pro94.09/98.8995.03/99.4594.11/99.4585.58/98.3494.48/99.4594.11/99.4585.56/97.2487.43/98.3487.29/98.34
RdRp94.80/99.2095.13/99.2094.40/98.4187.17/97.6193.87/99.2093.81/99.0088.47/98.8086.77/97.6185.51/97.41
ORF2VP192.22/97.8979.37/71.3269.02/70.9485.85/95.7991.78/96.7491.20/97.1386.93/94.4487.76/94.8386.62/95.98
ORF3VP291.55/94.4469.30/64.8167.88/65.7485.25/91.2091.72/94.9191.86/95.3786.33/90.2884.49/92.0685.43/92.59
DOI: https://doi.org/10.2478/jvetres-2024-0005 | Journal eISSN: 2450-8608
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Language: English
Page range: 1 - 8
Submitted on: Feb 13, 2023
Accepted on: Jan 25, 2024
Published on: Mar 23, 2024
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
calf diarrhoea,
bovine norovirus,
complete genome,
VP1
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2024 Zhigang Ma, Qian Jiang, Chenxi Quan, Lu Liu, Zhonghua Zhang, Jinxing Xie, Lu Zhao, Qi Zhong, Gang Yao, Xuelian Ma, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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