Fig. 1.
Fig. 2.
Fig. 3.
Fig. 4.
Fig. 5.
Fig. 6.
Primers1) used in this study for amplification of bovine herpesvirus 6 DNA fragments
| Primer | Genome position2) | Primer sequence (5’→3’) | Use |
|---|---|---|---|
| gBF | 35446-35467 | AACCTTATCCCGTACATGTTTC | PCR |
| gBR | 37722–37691 | CAAAGACCAACATGCCGCCAAA | PCR |
| gHF | 54713–56173 | GAGTCTGGCTTGAATGACGATC | PCR |
| gHR | 56021–54767 | GGGGTCAGTAATGCAGGGCCTA | PCR |
| pL52gBF3) | 35668–35689 | GGTTGGGAATTGCAATCTAACATCACGGTGGACCTTA | nested PCR, cloning |
| pL52gBR | 37056–37036 | GGAGATGGGAAGTCATTAGCTGCTTAAGCGCTTCTCTTC | nested PCR, cloning |
| pL52gHF | 54767–54787 | GGTTGGGAATTGCAATACAAAGTAGACAAAGAAGCT | nested PCR, cloning |
| pL52gHR4) | 56021–55090 | GGAGATGGGAAGTCATTATGATTCTTTGTCTACTTTGTA | nested PCR, cloning |
| LICfor55) | 296–316 | TAATACGACTCACTATAGGG | sequencing, colony PCR |
| LICrev | 559–538 | GAGCGGATAACAATTTCACAGG | sequencing, colony PCR |