Fig. 1.
Survival rate and fever evolution during experimentally induced African swine fever in 10-week-old Danbred Duroc pigs. Dots indicate environmental sampling timepoints. Red dots – before disinfection; green dot – after disinfection; dashed black line – fever threshold; dpi – days post infection
Fig. 2.
Estimated initial virus load in swab samples collected from 10-week-old Danbred Duroc pigs during experimentally induced African swine fever at 7 days post infection (dpi) and necropsy. The boxes represent the 50% between the 25 and 75% quartiles. The line inside the box indicates the median. The whiskers denote maximum and minimum values. eqTCID50 – 50% tissue culture infectious dose equivalent
Table 1.
Numbers of positive qPCR-samples in tested animal facility locations and their mean threshold cycle (Ct) values
| Time | Number of positive samples (Mean Ct (±SD)) | |||||||
|---|---|---|---|---|---|---|---|---|
| Floor | Wall | Door | HEPA filter cover | Drain | Feeder | Drinker | Mat | |
| T1 | 14/15 (35.9 (±1.6)) | 3/3 (33.3 (±1.9)) | 0/3 | 2/3 (37.3 (±0.4)) | 2/3 (28.7 (±1.4)) | 3/3 (34.3 (±1.9)) | 3/3 (33.0 (±0.8)) | 2/3 (38.3 (±0.6)) |
| T2 | 4/15 (38.2 (±0.6)) | 2/3 (36.7 (±2.2)) | 0/3 | 1/3 (36.7 (±0.0)) | 1/3 (32.4 (±0.2)) | 3/3 (35.9 (±1.2)) | 3/3 (36.7 (±1.6) | 3/3 (37.8 (±0.9)) |
| T3 | 5/15 (39.0 (±0.8)) | 2/3 (35.6 (±2.9)) | 0/3 | 1/3 (39.1 (±0.0)) | 1/3 (32.9 (±0.6)) | 3/3 (36.2 (±1.6)) | 3/3 (34.6 (±1.6)) | 3/3 (37.4 (±0.7)) |
Fig. 3.
Initial load of African swine fever virus in different pigsty locations after experimental infection and its change over time. Statistically significant decreases of estimated initial virus load were observed at 15 dpi at the floor and drain spots – locations mechanically cleaned by water. Error bars indicate standard deviation. eqTCID50 – 50% tissue culture infectious dose equivalent; HEPA – high-efficiency particulate-absorbing; * – statistically significant (P-value 0.002 (floor), 0.0393 (drain))