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Molecular contamination of an animal facility during and after African swine fever virus infection Cover

Molecular contamination of an animal facility during and after African swine fever virus infection

Journal of Veterinary Research
Volume 67 (2023): Issue 4 (December 2023)
By: Marek Walczak,  Krzesimir Szymankiewicz,  Fernando Rodriguez,  Jordi Argilaguet,  Boris Gavrilov,  Jacek Żmudzki,  Maciej Kochanowski,  Małgorzata Juszkiewicz and  Anna Szczotka-Bochniarz  
Open Access
|Dec 2023

Figures & Tables

Fig. 1.

Survival rate and fever evolution during experimentally induced African swine fever in 10-week-old Danbred Duroc pigs. Dots indicate environmental sampling timepoints. Red dots – before disinfection; green dot – after disinfection; dashed black line – fever threshold; dpi – days post infection
Survival rate and fever evolution during experimentally induced African swine fever in 10-week-old Danbred Duroc pigs. Dots indicate environmental sampling timepoints. Red dots – before disinfection; green dot – after disinfection; dashed black line – fever threshold; dpi – days post infection

Fig. 2.

Estimated initial virus load in swab samples collected from 10-week-old Danbred Duroc pigs during experimentally induced African swine fever at 7 days post infection (dpi) and necropsy. The boxes represent the 50% between the 25 and 75% quartiles. The line inside the box indicates the median. The whiskers denote maximum and minimum values. eqTCID50 – 50% tissue culture infectious dose equivalent
Estimated initial virus load in swab samples collected from 10-week-old Danbred Duroc pigs during experimentally induced African swine fever at 7 days post infection (dpi) and necropsy. The boxes represent the 50% between the 25 and 75% quartiles. The line inside the box indicates the median. The whiskers denote maximum and minimum values. eqTCID50 – 50% tissue culture infectious dose equivalent

Fig. 3.

Initial load of African swine fever virus in different pigsty locations after experimental infection and its change over time. Statistically significant decreases of estimated initial virus load were observed at 15 dpi at the floor and drain spots – locations mechanically cleaned by water. Error bars indicate standard deviation. eqTCID50 – 50% tissue culture infectious dose equivalent; HEPA – high-efficiency particulate-absorbing; * – statistically significant (P-value 0.002 (floor), 0.0393 (drain))
Initial load of African swine fever virus in different pigsty locations after experimental infection and its change over time. Statistically significant decreases of estimated initial virus load were observed at 15 dpi at the floor and drain spots – locations mechanically cleaned by water. Error bars indicate standard deviation. eqTCID50 – 50% tissue culture infectious dose equivalent; HEPA – high-efficiency particulate-absorbing; * – statistically significant (P-value 0.002 (floor), 0.0393 (drain))

Numbers of positive qPCR-samples in tested animal facility locations and their mean threshold cycle (Ct) values

TimeNumber of positive samples (Mean Ct (±SD))
FloorWallDoorHEPA filter coverDrainFeederDrinkerMat
T114/15(35.9 (±1.6))3/3(33.3 (±1.9))0/32/3(37.3 (±0.4))2/3(28.7 (±1.4))3/3(34.3 (±1.9))3/3(33.0 (±0.8))2/3(38.3 (±0.6))
T24/15(38.2 (±0.6))2/3(36.7 (±2.2))0/31/3(36.7 (±0.0))1/3(32.4 (±0.2))3/3(35.9 (±1.2))3/3(36.7 (±1.6)3/3(37.8 (±0.9))
T35/15(39.0 (±0.8))2/3(35.6 (±2.9))0/31/3(39.1 (±0.0))1/3(32.9 (±0.6))3/3(36.2 (±1.6))3/3(34.6 (±1.6))3/3(37.4 (±0.7))
DOI: https://doi.org/10.2478/jvetres-2023-0065 | Journal eISSN: 2450-8608
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Language: English
Page range: 503 - 508
Submitted on: Jul 18, 2023
|
Accepted on: Nov 14, 2023
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Published on: Dec 19, 2023
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
African swine fever,
pigsty,
indirect transmission,
persistence,
risk
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2023 Marek Walczak, Krzesimir Szymankiewicz, Fernando Rodriguez, Jordi Argilaguet, Boris Gavrilov, Jacek Żmudzki, Maciej Kochanowski, Małgorzata Juszkiewicz, Anna Szczotka-Bochniarz, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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