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The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK Cover

The serological and genetic diversity of the Leptospira interrogans Icterohaemorrhagiae serogroup circulating in the UK

Journal of Veterinary Research
Volume 67 (2023): Issue 4 (December 2023)
By: Zbigniew Arent,  Colm Gilmore,  Laura Pardyak,  Klaudia Dubniewicz,  Barry McInerney and  William Ellis  
Open Access
|Dec 2023

Figures & Tables

Fig. 1.

Restriction endonuclease analysis patterns of chromosomal DNA from Leptospira strains digested with EcoRI. Lanes: 1 – Icterohaemorrhagiae (RGA strain); 2 – Copenhageni (M20 strain); 3 – Monymusk (LT 75-68 strain); 4 – Budapest (PV-1 strain); 5 – Mankarso (Mankarso strain); 6 – Naam (Naam strain); 7 – Birkini (Birkin strain); 8 – Bogvere (LT 60-69 strain); 9 – Dakota (Grand River strain); 10 – Gem (Simon strain); 11 – Lai (Lai strain); 12 – Mwogolo (Mwogolo strain); 13 – Ndambari (Ndambari strain); 14 – Ndahambukuje (Ndahambukuje strain); 15 – Smithi (Smith strain); 16 – Tonkini (LT 96-68 strain)
Restriction endonuclease analysis patterns of chromosomal DNA from Leptospira strains digested with EcoRI. Lanes: 1 – Icterohaemorrhagiae (RGA strain); 2 – Copenhageni (M20 strain); 3 – Monymusk (LT 75-68 strain); 4 – Budapest (PV-1 strain); 5 – Mankarso (Mankarso strain); 6 – Naam (Naam strain); 7 – Birkini (Birkin strain); 8 – Bogvere (LT 60-69 strain); 9 – Dakota (Grand River strain); 10 – Gem (Simon strain); 11 – Lai (Lai strain); 12 – Mwogolo (Mwogolo strain); 13 – Ndambari (Ndambari strain); 14 – Ndahambukuje (Ndahambukuje strain); 15 – Smithi (Smith strain); 16 – Tonkini (LT 96-68 strain)

Fig. 2.

Unweighted pair-group method with arithmetic mean (UPGMA) tree based on the 12 variable-number tandem-repeat (VNTR) loci (V4, V7, V9, V10, V11, V19, V27, V29, V30, V31, V36 and V50). As only a partial repeat was present in V23 for all studied isolates, this locus was omitted in the UPGMA cluster analysis. Different VNTR profiles of 19 isolates revealed 5 distinct genotypes (A to E)
Unweighted pair-group method with arithmetic mean (UPGMA) tree based on the 12 variable-number tandem-repeat (VNTR) loci (V4, V7, V9, V10, V11, V19, V27, V29, V30, V31, V36 and V50). As only a partial repeat was present in V23 for all studied isolates, this locus was omitted in the UPGMA cluster analysis. Different VNTR profiles of 19 isolates revealed 5 distinct genotypes (A to E)

Reciprocals of agglutination titres of a panel of three monoclonal antibodies with reference strains of the Copenhageni (M20) and Icterohaemorrhagiae (RGA) serovars of Leptospira interrogans and two field strains of the bacteria_ The rest of the field strains gave the same results as those two representatives of each serovar

SerovarStrainMonoclonal antibody
F12C3F70C14F70C24
CopenhageniM2010,000-10,000
CopenhageniAB10210,000 10,000
IcterohaemorrhagiaeRGA30,00010,000-
IcterohaemorrhagiaeX24030,00010,000-

Source and origin characteristics of the Icterohaemorrhagiae serogroup Leptospira interrogans isolates used in this study

Strain identifierHostTissue of originClinical symptomsSerovarGenBank accession No.
23112HumanBloodAnorexia, feverCopenhageni
S72HorseFoetal kidneyAbortionCopenhageniGCA_017653945.1
X240DogKidneyAnorexia, fever, prostration, deathIcterohaemorrhagiaeGCA_017653925.1
R287CowFoetal kidneyAbortionCopenhageniGCA_017653725.1
AB102CowFoetal kidneyAbortionCopenhageni
S606CowFoetal lungAbortionCopenhageniGCA_017653855.1
W26/2HedgehogKidneyAsymptomaticCopenhageniGCA_017653845.1
B33BadgerKidneyAsymptomaticCopenhageniGCA_017653825.1
B42BadgerKidneyAsymptomaticCopenhageniGCA_017653785.1
B126BadgerKidneyAsymptomaticIcterohaemorrhagiaeGCA_017654005.1
B167BadgerKidneyAsymptomaticCopenhageniGCA_017653985.1
S1006BadgerKidneyAsymptomaticCopenhageniGCA_017654105.1
S199/2Brown ratKidneyAsymptomaticIcterohaemorrhagiaeGCA_017653805.1
S199/3Brown ratKidneyAsymptomaticCopenhageniGCA_017654025.1
S199/4Brown ratKidneyAsymptomaticCopenhageniGCA_017653755.1
S199/5Brown ratKidneyAsymptomaticCopenhageniGCA_017654055.1
S199/6Brown ratKidneyAsymptomaticCopenhageniGCA_017653965.1
S199/7Brown ratKidneyAsymptomaticCopenhageni
S199/12Brown ratKidneyAsymptomaticCopenhageniGCA_017654085.1

List of the Icterohaemorrhagiae serogroup Leptospira reference strains used in the restriction endonuclease analysis

SpeciesSerogroupSerovarStrainCountrySource
L. interrogansIcterohaemorrhagiaeIcterohaemorrhagiaeRGABelgiumHuman
L. interrogansIcterohaemorrhagiaeCopenhageniM20DenmarkHuman
L. interrogansIcterohaemorrhagiaeMonymuskLT 75-68JamaicaRat
L. interrogansIcterohaemorrhagiaeBudapestPV-1HungaryLaboratory rat
L. interrogansIcterohaemorrhagiaeMankarsoMankarsoIndonesiaHuman
L. interrogansIcterohaemorrhagiaeNaamNaamIndonesiaHuman
L. interrogansIcterohaemorrhagiaeBirkiniBirkinMalaysiaHuman
L. kirschneriIcterohaemorrhagiaeBogvereLT 60-69JamaicaRat
L. kirschneriIcterohaemorrhagiaeDakotaGrand RiverUSAWater
L. interrogansIcterohaemorrhagiaeGemSimonSri LankaHuman
L. interrogansIcterohaemorrhagiaeLaiLaiChinaHuman
L. kirschneriIcterohaemorrhagiaeMwogoloMwogoloZaireHuman
L. kirschneriIcterohaemorrhagiaeNdambariNdambariZaireHuman
L. kirschneriIcterohaemorrhagiaeNdahambukujeNdahambukujeZaireHuman
L. interrogansIcterohaemorrhagiaeSmithiSmithMalaysiaHuman
L. borgpeterseniiIcterohaemorrhagiaeTonkiniLT 96-68VietnamHuman
DOI: https://doi.org/10.2478/jvetres-2023-0063 | Journal eISSN: 2450-8608
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Language: English
Page range: 529 - 536
Submitted on: Jun 12, 2023
Accepted on: Nov 6, 2023
Published on: Dec 19, 2023
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
leptospirosis,
Icterohaemorrhagiae,
Copenhageni,
animal pathogens
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2023 Zbigniew Arent, Colm Gilmore, Laura Pardyak, Klaudia Dubniewicz, Barry McInerney, William Ellis, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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