Fig. 1.
Fig. 2.
Immunohistochemical results
| Germ cells/Spermatocytes | Sertoli cells | Leydig cells | ||||
|---|---|---|---|---|---|---|
| % of positive cells | Reaction intensity | % of positive cells | Reaction intensity | % of positive cells | Reaction intensity | |
| Normal mature testes | 4 | 3 | 4 | 3 | 3 | 2 |
| Atrophic areas of aged canine testes | 0 | 0 | 4 | 1 | 0 | 0 |
| Normal immature testes | 0 | 0 | 4 | 1 | 3 | 1 |
| Testes of miscarried foetuses | 0 | 0 | 2 | 1 | 0 | 0 |
Immunoreactive scores on the semiquantitative scale established by Remmele and Stegner (28)
| Percentage of positive cells | Points | Immunohistochemical reaction | Points |
|---|---|---|---|
| 0 | 0 | Absent | 0 |
| 1–10% | 1 | Weak | 1 |
| 11–50% | 2 | Moderate | 2 |
| 5–80% | 3 | Intense | 3 |
| >80% | 4 |