Table 1.
Real-time PCR results in fish experimentally infected with carp edema virus and maintained at 14°C (first iteration)
| Dpi | Fish number | Cycle threshold for a given tissue type | ||||||
|---|---|---|---|---|---|---|---|---|
| Brain | Gills | Kidney | Intestines | Skin | Spleen | |||
| 7 | 1 | +/40.60 | − | − | − | +/34.88 | − | |
| 2 | +/39.16 | +/38.62 | − | − | +/38.04 | − | ||
| 3 | +/39.79 | +/39.09 | − | +/40.77 | +/30.89 | − | ||
| 4 | − | +/39.31 | − | − | +/32.86 | − | ||
| 14 | 5 | +/40.56 | +/40.27 | − | − | +/35.84 | − | |
| 6 | − | +/38.56 | − | − | +/40.28 | − | ||
| 7 | +/40.11 | +/39.78 | +/41.29 | +/39.45 | +/38.49 | − | ||
| 21 | 8 | − | +/35.84 | +/40.09 | − | +/32.12 | − | |
| 9 | − | +/38.56 | − | − | +/40.67 | − | ||
| 10 | − | − | − | − | +/32.12 | − | ||
| 28 | 11 | − | +/39.67 | − | − | +/40.22 | − | |
| 12 | − | − | − | − | − | − | ||
Table 2.
Real-time PCR results in fish experimentally infected with carp edema virus and maintained at 14°C (second iteration)
| Dpi | Fish number | Cycle threshold for a given tissue type | ||||||
|---|---|---|---|---|---|---|---|---|
| Brain | Gills | Kidney | Intestines | Skin | Spleen | |||
| 7 | 1 | − | +/34.37 | − | − | +/29.98 | +/38.01 | |
| 2 | +/36.24 | +/36.06 | − | +/39.97 | +/24.19 | − | ||
| 3 | +/31.97 | +/34.36 | +/36.94 | +/40.06 | +/26.66 | − | ||
| 4 | − | +/38.13 | +/40.83 | − | +/32.54 | − | ||
| 14 | 5 | +/38.93 | +/39.12 | − | − | +/32.33 | − | |
| 6 | +/32.95 | +/33.74 | +/35.64 | − | +/28.10 | − | ||
| 7 | +/40.21 | +/38.52 | − | +/38.83 | +/37.18 | − | ||
| 21 | 8 | +/38.42 | +/37.75 | +/34.87 | +/37.97 | +/28.21 | − | |
| 9 | +/39.10 | +/35.67 | − | +/40.57 | +/36.79 | − | ||
| 10 | − | − | − | − | − | − | ||
| 28 | 11 | − | − | − | − | − | − | |
| 12 | − | − | − | − | +/33.79 | − | ||
Fig. 1.
Skin lesions (petechiae) in common carp fry experimentally infected with carp edema virus
Fig. 2.
Swollen gills and skin petechiae in common carp fry experimentally infected with carp edema virus
Fig. 3.
Percentages of positive real-time PCR results in tissue samples of carp edema virus–infected common carp fry