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First molecular detection of Brachyspira suanatina on pig farms in Poland Cover

First molecular detection of Brachyspira suanatina on pig farms in Poland

Journal of Veterinary Research
Volume 67 (2023): Issue 3 (September 2023)
By: Piotr Cybulski,  Katrin Strutzberg-Minder,  Edyta Michalik,  Radosław Kondratiuk and  Artur Jablonski  
Open Access
|Sep 2023

Figures & Tables

Farm descriptions

Finisher farmFarm capacity (thousand)Source of animals (weaner farm)Type of feedFinisher farm location (province)
F119W1pelletedWest Pomeranian
F29W2pelletedPomeranian
F313W3pelletedWest Pomeranian
F412W3pelletedWest Pomeranian
F514W4liquidWest Pomeranian
F611W2pelletedWest Pomeranian
F77W5pelletedWest Pomeranian
F821W6pelletedGreater Poland
F910W2pelletedWest Pomeranian

Detection of different enteric pathogens in the study

 Farm
PathogenAnalysisF1F2F3F4F5F6F7F8F9
Brachyspira spp.Brachyspira genusPCR+++++++++
B. hyodysenteriaePCR−−−−−−−−−
B. suanatinaPCR+++−+−+++
B. hampsoniiPCR−−−−−−−−−
B. pilosicoliPCR−+++−−−−+
B. murdochiiPCR−+−−++−++
B. intermediaPCR+−−−−−−−−
B. innocensPCR−−−−−−−−−
Salmonella spp.Salmonella sp.Culture−−SE−−SESESE−
Salmonella sp.PCRn/an/a+n/an/a+++n/a
Serovar CholeraesuisPCRn/an/a−n/an/a−+−n/a
Serovar TyphimuriumPCRn/an/a−n/an/a−−−n/a
VirusesTGEVPCR---------
PEDVPCR−−−−−−−−−
ParasitesHelminth eggs,Sedimentation−−−−−−−−−
encysted protozoaand flotation−−−−−−−−−
CryptosporidiaHeine staining−−−−−−−−−

Targets for detection and differentiation of Brachyspira species by PCR

Detection ofTarget geneReference
Brachyspira genus50S ribosomal protein L4(461 bp amplicon, mix 1)CP0196*
B. hyodysenteriaeBh100(194 bp amplicon)(32)
B. suanatinahypothetic protein(248 bp amplicon; mix 2)CVLB01000001*
B. hampsoniioxidoreductase(313 bp amplicon; mix 2)CP019914*
B. pilosicolimethyl-accepting chemotaxis protein B(128 bp amplicon; mix 1)CP002025*
B. murdochiihypothetic protein(362 bp amplicon; mix 2)CP001959*
B. intermediahypothetic protein(202 bp amplicon; mix 1)CP002874*
B. innocensribulokinase(259 bp amplicon; mix 1)ARQI01000130*
DOI: https://doi.org/10.2478/jvetres-2023-0038 | Journal eISSN: 2450-8608
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Language: English
Page range: 353 - 359
Submitted on: Mar 12, 2023
|
Accepted on: Jun 19, 2023
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Published on: Sep 20, 2023
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
spp.,
PCR,
pigs,
Poland,
swine dysentery
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2023 Piotr Cybulski, Katrin Strutzberg-Minder, Edyta Michalik, Radosław Kondratiuk, Artur Jablonski, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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