Table 1.
Dosages of the vaccine and sample size
| Group | Dose at one week of age | Dose at four weeks of age | Sample size |
|---|---|---|---|
| 1.0 + 1.0 | 1.0 mL | 1.0 mL | n = 14 |
| 0.5 + 1.0 | 0.5 mL | 1.0 mL | n = 14 |
| 0.5 + 0.5 | 0.5 mL | 0.5 mL | n = 14 |
| PV-Control | Not vaccinated | 1.0 mL | n = 12 |
| NV-Control | Not vaccinated | Not vaccinated | n = 12 |
Table 2.
Body mass index (BMI) values for the experimental and control groups of young Holstein calves and null hypothesis validity of the values
| Group | BMI | P-value | ||||
|---|---|---|---|---|---|---|
| 1.0 + 1.0 | 0.5 + 1.0 | 0.5 + 0.5 | PV-Control | NV-Control | ||
| 1.0 + 1.0 | 64.9 ± 0.8 | 1 | 0.898 | 1.000 | 0.731 | 0.715 |
| 0.5 + 1.0 | 64.0 ± 0.9 | 0.898 | 1 | 0.975 | 0.568 | 0.534 |
| 0.5 + 0.5 | 64.6 ± 0.8 | 1.000 | 0.975 | 1 | 0.731 | 0.584 |
| PV-Control | 66.8 ± 1.5 | 0.731 | 0.568 | 0.731 | 1 | 0.999 |
| NV-Control | 66.8 ± 1.7 | 0.715 | 0.534 | 0.584 | 0.999 | 1 |
Fig. 1.
Antibody responses in young Holstein calves vaccinated with a dose of 1.0 mL or 0.5 mL of an inactivated bacterial vaccine against bovine respiratory disease PV-Control - primary-vaccination control; NV-Control - no-vaccination control; arrows – vaccination times; **, * – significant increase compared to titre at one week of age (P < 0.01, P < 0.05); †† – significant decrease compared to titre at one week of age (P < 0.01); A-B, X-Y, a-b, x-y, α-β, χ-ψ – differences in final antibody titres (P < 0.01, P < 0.01, P < 0.05, P < 0.05, P < 0.10, and P < 0.10, respectively)
Table 3.
The number of calves with high and low maternal antibody levels and with positive and negative vaccine responses to (a) H. somni, (b) P. multocida and (c) M. haemolytica
| (a) | One week of age | Seven weeks of age | ||||
|---|---|---|---|---|---|---|
| H. somni | High MAL | Low MAL | P-value | Positive | Negative | P-value |
| 1.0 + 1.0 | 0 | 14 | − | 11 | 3 | 0.005** |
| 0.5 + 1.0 | 0 | 14 | − | 12 | 2 | 0.000** |
| 0.5 + 0.5 | 0 | 14 | − | 5 | 9 | 0.556 |
| PV-Control | 0 | 12 | − | 1 | 11 | 0.008** |
| NV-Control | 0 | 12 | − | 0 | 12 | 0.000** |
| P-value | 1.000 | 0.000** | ||||
| (b) | One week of age | Seven weeks of age | ||||
| P. multocida | High MAL | Low MAL | P-value | Positive | Negative | P-value |
| 1.0 + 1.0 | 9 | 5 | − | 14 | 0 | 0.015* |
| 0.5 + 1.0 | 4 | 10 | − | 14 | 0 | 0.015* |
| 0.5 + 0.5 | 6 | 8 | − | 13 | 1 | 0.159 |
| PV-Control | 7 | 5 | − | 5 | 7 | 0.006** |
| NV-Control | 6 | 6 | − | 4 | 8 | 0.000** |
| P-value | 0.402 | 0.000** | ||||
| (c) | One week of age | Seven weeks of age | ||||
| M. haemolytica | High MAL | Low MAL | P-value | Positive | Negative | P-value |
| 1.0 + 1.0 | 9 | 5 | − | 2 | 12 | − |
| 0.5 + 1.0 | 6 | 8 | − | 3 | 11 | − |
| 0.5 + 0.5 | 12 | 2 | − | 2 | 12 | − |
| PV-Control | 5 | 7 | − | 0 | 12 | − |
| NV-Control | 6 | 6 | − | 0 | 12 | − |
| P-value | 0.104 | 0.275 | ||||
Fig. 2.
Standardised regression coefficients from the logistic regression for seropositivity Primary – primary vaccination; Booster – booster vaccination; * – P < 0.05