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Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia Cover

Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia

Journal of Veterinary Research
Volume 67 (2023): Issue 2 (June 2023)
By: Jelena Maksimović Zorić,  Ljubiša Veljović,  Vladimir Radosavljević,  Dimitrije Glišić,  Jasna Kureljušić,  Jelena Maletić and  Božidar Savić  
Open Access
|Jun 2023

Figures & Tables

Fig. 1.

PB1-F2 amino acid sequences of Serbian swine influenza A virus strains in comparison to the sequence of A/California/04/2009reference strain. The red frames at positions 39, 46 and 51highlight methionine * – STOP codons
PB1-F2 amino acid sequences of Serbian swine influenza A virus strains in comparison to the sequence of A/California/04/2009reference strain. The red frames at positions 39, 46 and 51highlight methionine * – STOP codons

Fig. 2.

Partial amino acid sequences of basic polymerase protein 1 of Serbian swine influenza A virus strains. The red frame at position 40 indicates methionine
Partial amino acid sequences of basic polymerase protein 1 of Serbian swine influenza A virus strains. The red frame at position 40 indicates methionine

Fig. 3.

Partial amino acid sequences of the acidic polymerase protein of Serbian swine influenza A virus strains. The red frameat position 38 indicates isoleucine and those at positions 155 and 182 highlight methionine
Partial amino acid sequences of the acidic polymerase protein of Serbian swine influenza A virus strains. The red frameat position 38 indicates isoleucine and those at positions 155 and 182 highlight methionine

Fig. 4.

Amino acid sequences of the PA-X protein of Serbian swine influenza A virus strains * – STOP codons
Amino acid sequences of the PA-X protein of Serbian swine influenza A virus strains * – STOP codons

Analysed amino acid mutations within proteins of Serbian swine influenza A virus strains and their significance

ProteinAnalysed mutation/amino acid positionSignificanceReferences
PB2E627K• Polymerase activity in mammalian cells5, 15, 39
D701N• Polymerase activity in mammalian cells
G590S/Q591R• Polymerase activity in mammalian cells
T271A• Enhanced polymerase activity and virus growth of H1N1pdm09 viruses in mammalian hosts
D9N• B-interferon inhibition in mitochondria
R251K• Virulence of H1avN1 viruses
PAI38T• Antiviral drug (baloxavir marboxil) resistance35
HASa (128, 129, 158, 160, 162–167)• Antigenic drift7, 42
Sb (156, 159 187–198)
Ca1 (169–173 206–208 238–240)
Ca2 (140–145 224–225)
Cb (74–79, 83,122)
NPN313, V100I, 350K/T, 371V/M, 444V/I, 456L/V• Lineage characteristics34, 45
NAE119G/V, Q136K/R, K150T, D199G, I223K/R/V, S247N, H275Y, N295S• Resistance to neuraminidase inhibitors (oseltamivir, zanamivir, peramivir)6, 16, 19
M2L26F/I, V27A/T, A30T/V, S31N, G34E, L38F• Resistance to adamantane M2 ion channel inhibitors (amantadine, rimantadine)11
NS1D125G• Adaptation to new host species, synthesis of NS3 protein40
K108R, D125E, D189G• Antiviral activity of host cells12

Results of the comparison of haemagglutinin (HA) antigenic determinants between Serbian swine influenza A virus strains and vaccine strains

SRB/sw/2017/2pdm SRB/sw/2016/3pdm
Vaccine strainH1 HA antigenic siteAmino acid substitution
A/Jena/VI5258/2009 (H1N1pdm09)SaG158V, L164I N129D, G158V, L164I
Sb- -
Ca1- S206T
Ca2S146G A144E
Cb- -
SRB/sw/2017/1avSRB/sw/2016/5avSRB/sw/2017/7av
H1 HA antigenic siteAmino acid substitution
A/swine/Haselünne/2617/2003 (H1avN1)Sa K166IN129E, S165N
Sb N159G, Q192L
Ca1 T169AQ239K
Ca2H141Y, G225EA144S, G225EA144T, G225E
Cb L78F

Genotype composition of Serbian swine influenza A viruses

Virus (subtype)AbbreviationGenotype
PB2PB1PAHANPNAMNS
A/swine/Serbia/1/2017(H1N1)SRB/sw/2017/1
A/swine/Serbia/2/2017(H1N1)SRB/sw/2017/2
A/swine/Serbia/3/2016(H1N1)SRB/sw/2016/3
A/swine/Serbia/5/2016(H1N1)SRB/sw/2016/5
A/swine/Serbia/7/2017(H1N1)SRB/sw/2017/7
DOI: https://doi.org/10.2478/jvetres-2023-0034 | Journal eISSN: 2450-8608
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Language: English
Page range: 147 - 154
Submitted on: Feb 7, 2023
Accepted on: Jun 2, 2023
Published on: Jun 16, 2023
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
swine influenza A viruses,
molecular markers,
accessory proteins,
antigenicity,
drug resistance
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2023 Jelena Maksimović Zorić, Ljubiša Veljović, Vladimir Radosavljević, Dimitrije Glišić, Jasna Kureljušić, Jelena Maletić, Božidar Savić, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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