Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia

Virus (subtype)	Abbreviation	Genotype
Virus (subtype)	Abbreviation	PB2	PB1	PA	HA	NP	NA	M	NS
A/swine/Serbia/1/2017(H1N1)	SRB/sw/2017/1
A/swine/Serbia/2/2017(H1N1)	SRB/sw/2017/2
A/swine/Serbia/3/2016(H1N1)	SRB/sw/2016/3
A/swine/Serbia/5/2016(H1N1)	SRB/sw/2016/5
A/swine/Serbia/7/2017(H1N1)	SRB/sw/2017/7

1 blue – H1avN1 lineage; red – H1N1pdm09 lineage

Table 2.

Analysed amino acid mutations within proteins of Serbian swine influenza A virus strains and their significance

Protein	Analysed mutation/amino acid position	Significance	References
PB2	E627K	• Polymerase activity in mammalian cells	5, 15, 39
	D701N	• Polymerase activity in mammalian cells
	G590S/Q591R	• Polymerase activity in mammalian cells
	T271A	• Enhanced polymerase activity and virus growth of H1N1pdm09 viruses in mammalian hosts
	D9N	• B-interferon inhibition in mitochondria
	R251K	• Virulence of H1avN1 viruses
PA	I38T	• Antiviral drug (baloxavir marboxil) resistance	35
HA	Sa (128, 129, 158, 160, 162–167)	• Antigenic drift	7, 42
	Sb (156, 159 187–198)
	Ca1 (169–173 206–208 238–240)
	Ca2 (140–145 224–225)
	Cb (74–79, 83,122)
NP	N313, V100I, 350K/T, 371V/M, 444V/I, 456L/V	• Lineage characteristics	34, 45
NA	E119G/V, Q136K/R, K150T, D199G, I223K/R/V, S247N, H275Y, N295S	• Resistance to neuraminidase inhibitors (oseltamivir, zanamivir, peramivir)	6, 16, 19
M2	L26F/I, V27A/T, A30T/V, S31N, G34E, L38F	• Resistance to adamantane M2 ion channel inhibitors (amantadine, rimantadine)	11
NS1	D125G	• Adaptation to new host species, synthesis of NS3 protein	40
NS1	K108R, D125E, D189G	• Antiviral activity of host cells	12

1 PB2 – basic polymerase 2; PA – acidic polymerase; HA – haemagglutinin; NP – nucleoprotein; NA – neuraminidase; M2 – matrix 2 protein; NS1 – non-structural protein 1

PB1-F2 amino acid sequences of Serbian swine influenza A virus strains in comparison to the sequence of A/California/04/2009reference strain. The red frames at positions 39, 46 and 51highlight methionine
* – STOP codons

Partial amino acid sequences of basic polymerase protein 1 of Serbian swine influenza A virus strains. The red frame at position 40 indicates methionine

Partial amino acid sequences of the acidic polymerase protein of Serbian swine influenza A virus strains. The red frameat position 38 indicates isoleucine and those at positions 155 and 182 highlight methionine

Amino acid sequences of the PA-X protein of Serbian swine influenza A virus strains
* – STOP codons

Table 3.

Results of the comparison of haemagglutinin (HA) antigenic determinants between Serbian swine influenza A virus strains and vaccine strains

		SRB/sw/2017/2^pdm		SRB/sw/2016/3^pdm
Vaccine strain	H1 HA antigenic site	Amino acid substitution
A/Jena/VI5258/2009 (H1N1pdm09)	Sa	G158V, L164I		N129D, G158V, L164I
	Sb	-		-
	Ca1	-		S206T
	Ca2	S146G		A144E
	Cb	-		-
		SRB/sw/2017/1^av	SRB/sw/2016/5^av	SRB/sw/2017/7^av
	H1 HA antigenic site	Amino acid substitution
A/swine/Haselünne/2617/2003 (H1avN1)	Sa		K166I	N129E, S165N
	Sb		N159G, Q192L
	Ca1		T169A	Q239K
	Ca2	H141Y, G225E	A144S, G225E	A144T, G225E
	Cb		L78F

1 ^pdm–HA gene of H1N1pdm09 lineage; ^av– HA gene of H1avN1 lineage

Articles in this issue

DOI: https://doi.org/10.2478/jvetres-2023-0034 | Journal eISSN: 2450-8608 (formerly 2300-3235)

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Language: English

Page range: 147 - 154

Submitted on: Feb 7, 2023

Accepted on: Jun 2, 2023

Published on: Jun 16, 2023

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

swine influenza A viruses,

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2023 Jelena Maksimović Zorić, Ljubiša Veljović, Vladimir Radosavljević, Dimitrije Glišić, Jasna Kureljušić, Jelena Maletić, Božidar Savić, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 67 (2023): Issue 2 (June 2023)

Protein sequence features of H1N1 swine influenza A viruses detected on commercial swine farms in Serbia

Figures & Tables

Table 1.

Table 2.

Fig. 1.

Fig. 2.

Fig. 3.

Fig. 4.

Table 3.

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