Analysis of the effect of polymorphisms within the CATHL7 gene on dairy performance parameters

Figures & Tables

SNP	PCR Conditions	Product size	Restriction enzyme	Genotype	Fragment size after digestion
	initial: 95ºC/5 min			CC	324, 23 bp
2,383C > G			HhaI	CG	347, 324, 23 bp
	35 cycles: denaturation:95ºC/30 s			GG	347 bp
	annealing: 52ºC/45 s extension:72ºC/30 s	347 bp		CC	161, 81, 58, 34, 14 bp
2,468 C > G			HinfI	CG	219, 161, 81, 58, 34, 13 bp
	final extension: 72ºC/7 min			GG	219, 81, 34, 13 bp

N	Genotype	Milk yield kg	Fat content %	Protein content %	Lactose content %	LnSCC
			CATHL7/HhaI
138	CC	33.93^A ± 7.86	3.84^A ± 0.72	3.52^A ± 0.33	4.95^AB ± 0.16	4.18^A ± 0.82
68	CG	33.81^B ± 8.18	3.80^B ± 0.75	3.52^B ± 0.35	4.89^B ± 0.17	4.28 ± 0.84
17	GG	30.42^AB ± 6.29	4.07^AB ± 0.70	3.59^AB ± 0.37	4.90^A ± 0.16	4.34^A ± 0.75
			CATHL7/HinfI
117	CC	33.48 ± 7.59	3.87 ± 0.71	3.53^A ± 0.34	4.94^a ± 0.16	4.18 ± 0.85
91	CG	33.87 ± 8.34	3.81 ± 0.77	3.51^a ± 0.34	4.91^a ± 0.16	4.27 ± 0.81
15	GG	34.42 ± 7.89	3.80 ± 0.67	3.47^Aa ± 0.31	4.92 ± 0.17	4.25 ± 0.73

	N	Genotype frequencies		Allele frequencies		χ²
	169	CC	0.606
CATHL7/HhaI	91	CG	0.326	C	0.769	1.897
	19	GG	0.068	G	0.231
	144	CC	0.516
CATHL7/HinfI	115	CG	0.412	C	0.722	0.208
	20	GG	0.072	G	0.278