Table 1
PCR conditions, product size, restriction enzyme, and fragment size after restriction endonuclease digestion
| SNP | PCR Conditions | Product size | Restriction enzyme | Genotype | Fragment size after digestion |
|---|
Fig. 1
Agarose gel electrophoresis (3%) of PCR-restriction fragment length polymorphism result for CATHL7/HhaI polymorphism. From left: pUC19/MspI DNA ladder (501/489, 404, 331, 242, 190, 147, 111/110, 67 and 34 base pairs (bp)), CC genotype (324 and 23 bp), CG genotype (347, 324 and 23 bp), and GG genotype (347 bp); the 23 bp fragment is not visible because of its size and diffusion in gel
Fig. 2
Agarose gel electrophoresis (3%) of PCR-restriction fragment length polymorphism result for CATHL7/HinfI polymorphism. From left: pUC19/MspI DNA ladder (501/489, 404, 331, 242, 190, 147, 111/110, 67 and 34 base pairs (bp)), CC genotype (161, 81, 58, 34 and 14 bp), CG genotype (219, 161, 81, 58, 34 and 13 bp), and GG genotype (219, 81, 34 and 13 bp)
Table 2
Genotype and allele frequencies of CATHL7
| N | Genotype frequencies | Allele frequencies | χ2 | |||
|---|---|---|---|---|---|---|
| 169 | CC | 0.606 | ||||
| 144 | CC | 0.516 | ||||
| CATHL7/HinfI | 115 | CG | 0.412 | C | 0.722 | 0.208 |
| 20 | GG | 0.072 | G | 0.278 | ||
Table 3
Means and standard deviation of traits in relation to genotypes
| N | Genotype | Milk yield kg | Fat content % | Protein content % | Lactose content % | LnSCC |
|---|---|---|---|---|---|---|
| 138 | CC | 33.93A ± 7.86 | 3.84A ± 0.72 | 3.52A ± 0.33 | 4.95AB ± 0.16 | 4.18A ± 0.82 |
| 68 | CG | 33.81B ± 8.18 | 3.80B ± 0.75 | 3.52B ± 0.35 | 4.89B ± 0.17 | 4.28 ± 0.84 |
| 117 | CC | 33.48 ± 7.59 | 3.87 ± 0.71 | 3.53A ± 0.34 | 4.94a ± 0.16 | 4.18 ± 0.85 |
| 91 | CG | 33.87 ± 8.34 | 3.81 ± 0.77 | 3.51a ± 0.34 | 4.91a ± 0.16 | 4.27 ± 0.81 |
| 15 | GG | 34.42 ± 7.89 | 3.80 ± 0.67 | 3.47Aa ± 0.31 | 4.92 ± 0.17 | 4.25 ± 0.73 |