Fig. 1
Sensitivity and specificity of iodine, acid-fast stain, trichrome and culture techniques
| Polymerase chain reaction (gold standard) | Sensitivity | Specificity | Odds ratio | 95% Confidence interval | P-value | ||||
|---|---|---|---|---|---|---|---|---|---|
| Positive (n = 5) | Negative (n = 60) | Lower | Upper | ||||||
| Iodine | Positive | 13.3 (2) | 86.7 (13) | 0.4 | 0.783 | 2.41 | 0.36 | 15.98 | 0.35 |
| Negative | 6 (3) | 94 (47) | |||||||
| Acid-fast | Positive | 16.7 (2) | 83.3 (10) | 0.4 | 0.833 | 3.33 | 0.49 | 22.59 | 0.19 |
| stain | Negative | 5.7 (3) | 94.3 (50) | ||||||
| Trichrome | Positive | 25 (4) | 75 (12) | 0.8 | 0.800 | 16 | 1.63 | 156.5 | 0.003 |
| Negative | 2 (1) | 98 (48) | |||||||
| Positive | 22.2 (4) | 77.8 (14) | |||||||
| Culture | Negative | 2.1 (1) | 97.9 (46) | 0.8 | 0.767 | 13.14 | 1.35 | 127.4 | 0.007 |
GenBank DNA BLAST sequence of Blastocystis in sheep (present study) compared to Blastocystis ST10 isolate CA (KC148207)
| Isolate | GenBank ID | Maximal score | Total score | Query cover (%) | Identity (%) |
|---|---|---|---|---|---|
| 1 | MT895705 | 571 | 571 | 94 | 85.41 |
| 2 | MT895706 | No score | No score | No score | No score |
| 3 | MT895707 | 407 | 407 | 87 | 81.01 |
| 5B | MT895708 | No score | No score | No score | No score |
| 6 | MT895709 | No score | No score | No score | No score |
| 7 | MT895710 | No score | No score | No score | No score |
| 8 | MT895711 | 540 | 540 | 94 | 84.20 |
| 9 | MT895712 | 512 | 512 | 95 | 87.33 |
| 10 | MT895713 | No score | No score | No score | No score |
| 12 | MT895714 | 560 | 560 | 99 | 86.15 |
| 4 | MT895715 | 968 | 968 | 97 | 97.03 |
| 5A | MT895716 | 1016 | 1016 | 97 | 99.11 |