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Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle Cover

Assessment of tuberculosis biomarkers in paratuberculosis-infected cattle

Open Access
|Mar 2023

Figures & Tables

Fig. 1

Fold changes of gene transcription in PBMCs from PTB+ and healthy animals upon PPDa stimulation. Fold change values from individual animals are represented by squares (PTB+ animals) and circles (healthy animals). Median and interquartile ranges are shown. Relative gene transcription was calculated with the 2-ΔΔCt method with E correction and using glyceraldehyde 3-phosphate dehydrogenase mRNA as the reference gene and the non-stimulated condition as the calibrator. Data were analysed using a two-tailed unpaired Mann-Whitney test CD14 – Cluster of differentiation 14; CXCL10 – C-X-C motif chemokine ligand 10; PTB – paratuberculosis; IFN-γ – interferon gamma; IL-1R – interleukin 1 receptor; IL-22 – interleukin 22; MMP9 – matrix metallopeptidase 9; THBS1 – thrombospondin 1; *** – P < 0.001
Fold changes of gene transcription in PBMCs from PTB+ and healthy animals upon PPDa stimulation. Fold change values from individual animals are represented by squares (PTB+ animals) and circles (healthy animals). Median and interquartile ranges are shown. Relative gene transcription was calculated with the 2-ΔΔCt method with E correction and using glyceraldehyde 3-phosphate dehydrogenase mRNA as the reference gene and the non-stimulated condition as the calibrator. Data were analysed using a two-tailed unpaired Mann-Whitney test CD14 – Cluster of differentiation 14; CXCL10 – C-X-C motif chemokine ligand 10; PTB – paratuberculosis; IFN-γ – interferon gamma; IL-1R – interleukin 1 receptor; IL-22 – interleukin 22; MMP9 – matrix metallopeptidase 9; THBS1 – thrombospondin 1; *** – P < 0.001

Results of ELISA, PCR test in faecal material and fold change transcription of target genes of animals used in this study

Animal IDELISAPCRIFN-γIL-22CXCL10THBS1IL-1RMMP9CD14
1++3.684.276.10−7.44−2.28−2.73−1.25
2++−2.962.84−3.20−10.98−4.09−3.05−3.35
3+1.316.345.56−17.34−1.31−1.79−2.97
4++2.999.1921.761.493.511.57−1.89
5+1.747.521.65−8.02−1.391.40−1.93
6++−1.114.63−1.48−1.961.402.201.21
7+4.27288.7433.97−58.892.525.651.01
8+1.41317.418.63−44.741.973.26−1.77
9+−1.215.59−2.17−11.42−2.48−4.06−3.15
10++−1.171.422.86−5.071.161.251.11
11+9.001.9326.86−4.56−1.363.071.20
12++−2.40−219.178.20−4.811.67−3.021.11
13+17.13389.8011.52−4.281.881.55−2.38
142.548.4325.62−1.581.47−1.01−1.90
152.2016.743.7112.231.45−1.541.22
161.4422.231.05−1.212.321.981.26
17−2.44−1.14−1.19−5.36−1.03−2.41−2.91
18−7.21−2.51−2.1610.831.792.95−1.09
19−2.371.111.47−1.653.721.70−1.65
206.0756.4520.35−2.53−1.45−2.05−1.46
213.4627.6315.70−1.65−1.25−1.60−1.10
225.8514.4417.66−1.56−1.27−2.25−2.44
23−2.94ND−1.02−2.355.601.55−1.38
246.2927.911.7510.666.986.935.50
252.4236.691.732.882.781.321.25
26−1.453.81−1.49−1.07−1.32−1.64−1.31
2711.6745.129.14−3.9−1.55−2.84−1.98
Language: English
Page range: 55 - 60
Submitted on: Jul 27, 2022
Accepted on: Feb 10, 2023
Published on: Mar 9, 2023
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2023 Laura I. Klepp, María A. Colombatti, Roberto D. Moyano, María I. Romano, Tadej Malovrh, Matjaž Ocepek, Federico C. Blanco, Fabiana Bigi, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.