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Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams Cover

Longitudinal study on seroreactivity of goats exposed to colostrum and milk of small ruminant lentivirus–infected dams

Journal of Veterinary Research
Volume 66 (2022): Issue 4 (December 2022)
By: Jarosław Kaba,  Michał Czopowicz,  Lucjan Witkowski,  Olga Szaluś-Jordanow,  Marcin Mickiewicz,  Iwona Markowska-Daniel,  Ryszard Puchała and  Emilia Bagnicka  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Timeline of the serological status of 13 goats that seroconverted during the study. The orange band is the duration of maternal antibodies, the blue band is the time of seronegativity in both ELISAs, and the red band is the time of seropositivity in at least one ELISA. The direct transition from the period of maternal antibodies to the period of active seroreactivity by goat no. 28 results from the overlapping seropositivities of this goat in the two ELISAs used – see Fig. 3 for details
Timeline of the serological status of 13 goats that seroconverted during the study. The orange band is the duration of maternal antibodies, the blue band is the time of seronegativity in both ELISAs, and the red band is the time of seropositivity in at least one ELISA. The direct transition from the period of maternal antibodies to the period of active seroreactivity by goat no. 28 results from the overlapping seropositivities of this goat in the two ELISAs used – see Fig. 3 for details

Fig. 2

Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in intermittent (goat no. 9 and goat no. 18) and late seroreactors (goat no. 13 and goat no. 20). Dashed lines indicate the manufacturer’s cut-off value used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of the sp-iELISA (red) and the SU-cELISA (green) or the times at which an intermittent seroreactor was seropositive
Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in intermittent (goat no. 9 and goat no. 18) and late seroreactors (goat no. 13 and goat no. 20). Dashed lines indicate the manufacturer’s cut-off value used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of the sp-iELISA (red) and the SU-cELISA (green) or the times at which an intermittent seroreactor was seropositive

Fig. 3

Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in early and stable seroreactors. Dashed lines indicate the manufacturer’s cut-off values used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of sp-iELISA (red) and SU-cELISA (green)
Quantitative results of an indirect ELISA based on synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) and a competitive ELISA based on surface glycoprotein (SU-cELISA; percent inhibition, PI) in early and stable seroreactors. Dashed lines indicate the manufacturer’s cut-off values used to interpret the results of the sp-iELISA (red; S/P% = 50%) and the SU-cELISA (green; PI = 35%). The arrows indicate the first positive result of sp-iELISA (red) and SU-cELISA (green)

Fig. 4

Quantitative results of an indirect ELISA coated with the mixture of synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) of 18 seronegative goats in the first 24 months of their lives. The red dashed line indicates the manufacturer’s cut-off value of S/P% = 50%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months
Quantitative results of an indirect ELISA coated with the mixture of synthetic viral peptides (sp-iELISA; sample-to-positive control serum ratio, S/P%) of 18 seronegative goats in the first 24 months of their lives. The red dashed line indicates the manufacturer’s cut-off value of S/P% = 50%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months

Fig. 5

Quantitative results of a competitive ELISA coated with viral surface glycoprotein (SU-cELISA; percent inhibition, PI) of 18 seronegative goats in the first 24 months of their lives. The green dashed line indicates the manufacturer’s cut-off value of PI = 35%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months
Quantitative results of a competitive ELISA coated with viral surface glycoprotein (SU-cELISA; percent inhibition, PI) of 18 seronegative goats in the first 24 months of their lives. The green dashed line indicates the manufacturer’s cut-off value of PI = 35%. The dark solid line under the x axis indicates the time period in which maternal (colostral) antibodies were detected. The asterisk (*) indicates significant difference (α=0.05) compared to the age of 4 months

DamNumber of kids born in a particular year with number of seroreactors in parentheses Year the dam was bornYear the dam seroconverted or first tested seropositive
20142015
11 (0)-20052007
21 (0)-20092011
31 (0)1 (0)20092012
4-2 (2a)20092014
52 (0)-20102011
61 (1a)-20102013
72 (1c)-20062013
81 (0)-20112013
91 (1c)-20112013
101 (0)-20112013
11-2 (1a)20122014
121 (1b)2 (2a)20082013
132-20072010
141 (0)-20082010
151 (0)-20082013
162 (1b)2 (1a)20082013
172 (1a)2 (1a)20092013
Total20 (2a, 2b, 2c)11 (7 a)
DOI: https://doi.org/10.2478/jvetres-2022-0071 | Journal eISSN: 2450-8608
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Language: English
Page range: 511 - 521
Submitted on: Sep 13, 2022
Accepted on: Dec 7, 2022
Published on: Dec 26, 2022
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
caprine arthritis-encephalitis,
colostral antibodies,
humoral immunity,
maternal antibodies,
seroconversion
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2022 Jarosław Kaba, Michał Czopowicz, Lucjan Witkowski, Olga Szaluś-Jordanow, Marcin Mickiewicz, Iwona Markowska-Daniel, Ryszard Puchała, Emilia Bagnicka, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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