Fig. 1
Fig. 2
Summary of mink farm SARS-CoV-2 monitoring data amassed during a period of 14 months
| Voivodeship | 2021 (February–December) | 2022 (January–March) | Total | Number of positive farms | |||
|---|---|---|---|---|---|---|---|
| No. of farms | No. of swabs | No. of farms | No. of swabs | No. of farms | No. of swabs | ||
| Mazowieckie | 58 | 1,160 | 28 | 420 | 86 | 1,580 | 0 |
| Podkarpackie | 8 | 160 | 5 | 75 | 13 | 235 | 1 |
| Lubuskie | 35 | 700 | 19 | 295 | 54 | 995 | 0 |
| Wielkopolskie | 117 | 2,340 | 87 | 1,472 | 204 | 3,812 | 1 |
| Zachodniopomorskie | 54 | 1,080 | 8 | 420 | 62 | 1,500 | 3 |
| Pomorskie | 19 | 380 | 1 | 20 | 20 | 400 | 1 |
| Dolnośląskie | 14 | 280 | 0 | 0 | 14 | 280 | 0 |
| Kujawsko-Pomorskie | 24 | 480 | 0 | 0 | 24 | 480 | 1 |
| Lubelskie | 36 | 1,120 | 22 | 356 | 58 | 1,476 | 2 |
| Łódzkie | 6 | 120 | 1 | 15 | 7 | 135 | 1 |
| Małopolskie | 7 | 140 | 1 | 15 | 8 | 155 | 0 |
| Opolskie | 6 | 120 | 0 | 0 | 6 | 120 | 0 |
| Podlaskie | 10 | 200 | 0 | 0 | 10 | 200 | 1 |
| Świętokrzyskie | 6 | 120 | 13 | 185 | 19 | 305 | 0 |
| Warmińsko-Mazurskie | 1 | 20 | 0 | 0 | 1 | 20 | 0 |
| Śląskie | 8 | 160 | 0 | 0 | 8 | 160 | 0 |
| Total | 409 | 8,580 | 185 | 3,273 | 594 | 11,853 | 11 |
A summary of the analysis of positive mink farms
| rRT-PCR | |||||
|---|---|---|---|---|---|
| No | Date of sample collection | Voivodeship | Location | Positive/Tested | Ct value range* |
| Wisznice (house 1) | 2/20 | 21.8–25.5 | |||
| 1 | 16/06/2021 | Lubelskie | Wisznice (house 2) | 1/20 | 28.9 |
| 2 | 22/11/2021 | Kujawsko-Pomorskie | Kraczki | 1/20 | 28.9 |
| 3 | Zieleniewo1 | 1/20 | 30.5 | ||
| 4 | 30/11/2021 | Zachodniopomorskie | Zieleniewo2 | 13/20 | 24.7–36.8 |
| 5 | Ołużna | 19/20 | 18.9–37.3 | ||
| 6 | 01/12/2021 | Wielkopolskie | Biadki | 18/20 | 17.6–36.4 |
| 7 | 14/12/2021 | Podkarpackie | Janowiec | 20/20 | 28.1–30.4 |
| 8 | 15/12/2021 | Podlaskie | Kościuki | 2/20 | 32.1–35.3 |
| 9 | 20/12/2021 | Pomorskie | Leźno | 10/20 | 19.1–26.3 |
| 10 | 20/12/2021 | Lubelskie | Kłoczew | 5/20 | 28.9–30.2 |
| 11 | 20/01/2022 | Łódzkie | Stefanów | 2/20 | 20.5–21.8 |
Molecular characteristics of SARS-CoV-2 detected in farmed mink in Poland in the studied period
| No | Mink farm | Gisaid_ID | Clade | Pango lineage | Nucleotide | Frame shifts | Amino acid | ||
|---|---|---|---|---|---|---|---|---|---|
| substit. | delet. | substit. | delet. | ||||||
| 1 | Wisznice, house 2, | EPI_ISL_3218555 | GR/20B | B.1.1 | 31 | 0 | 0 | 18 | 0 |
| Lubelskie | EPI_ISL_3218557 | 31 | 0 | 0 | 18 | 0 | |||
| 2 | Kraczki, Kujawsko-Pomorskie | EPI_ISL_7721854 | GK/21J (Delta) | AY.43 | 44 | 13 | 0 | 34 | 4 |
| EPI_ISL_8693906 | 38 | 13 | 0 | 33 | 4 | ||||
| 3 | Ołużna, Zachodniopomorskie | EPI_ISL_8693911 | GK/21J (Delta) | AY.43 | 40 | 14 | 1 | 34 | 4 |
| EPI_ISL_8693912 | 38 | 13 | 0 | 33 | 4 | ||||
| 4 | ZieleniewoZachodniopomorskie 1, | EPI_ISL_8693816 | GK/21J (Delta) | AY.43 | 38 | 13 | 0 | 33 | 4 |
| EPI_ISL_8693913 | 41 | 13 | 0 | 34 | 4 | ||||
| 5 | Biadki, Wielkopolskie | EPI_ISL_8693914 | GK/21J (Delta) | AY.43 | 41 | 13 | 0 | 34 | 4 |
| EPI_ISL_8693915 | 41 | 13 | 0 | 34 | 4 | ||||
| EPI_ISL_9640028 | 51 | 104 | 1 | 37 | 34 | ||||
| 6 | Janowiec, Podkarpackie | EPI_ISL_9640033 | GK/21J (Delta) | AY.126 | 50 | 107 | 1 | 37 | 34 |
| EPI_ISL_9640052 | 50 | 107 | 1 | 37 | 34 | ||||
| 7 | Kościuki, Podlaskie | EPI_ISL_9640055 | GRY/20I (Alpha, V1) | B.1.1.7 | 52 | 37 | 0 | 30 | 11 |
| 8 | Leźno, Pomorskie | EPI_ISL_9640059 | GK/21J (Delta) | B.1.617.2 | 47 | 16 | 0 | 37 | 5 |
| EPI_ISL_9640062 | 45 | 47 | 1 | 36 | 12 | ||||
| 9 | Kłoczew, Lubelskie | EPI_ISL_9640065 | GK/21J (Delta) | AY.122 | 41 | 16 | 0 | 32 | 4 |
| EPI_ISL_10337406 | 74 | 53 | 0 | 51 | 12 | ||||
| 10 | Stefanów, Łódzkie | EPI_ISL_10337127 | GRA/21L (Omicron) | BA.2 | 72 | 53 | 0 | 51 | 12 |
Sequence mutations in SARS-CoV-2 detected in farmed mink in Poland in the studied period
| No | Mink farm | Pango lineage | Nonsynonymous mutations in the S protein in comparison to the Wuhan reference strain |
|---|---|---|---|
| 1 | Wisznice, Lubelskie house 2, | B.1.1 | Q183R, Y453F, K558N, D614G, C1236F |
| 2 | Kraczki, Kujawsko- Pomorskie | AY.43 | T19R, E96A, G142D, R158G, L452R, T478K, D614G, P681R, D950N, E156-, F157- |
| 3 | Ołużna, Zachodniopomorskie | AY.43 | T19R, G142D, R158G, L452R, T478K, D614G, P681R, D950N, E156-, F157- |
| 4 | Zieleniewo1, Zachodniopomorskie | AY.43 | T19R, G142D, R158G, L452R, T478K, D614G, P681R, D950N, E156-, F157- |
| 5 | Biadki, Wielkopolskie | AY.43 | T19R, G142D, R158G, L452R, T478K, D614G, P681R, D950N, E156-, F157- |
| 6 | Janowiec, Podkarpackie | AY.126 | T19R, T95I, G142D, R158G, G181V, L452R, T478K, N532S, D614G, P681R, I850L, D950N, K1045N E156-, F157- |
| 7 | Kościuki, Podlaskie | B.1.1.7 | S94F, Y453F, N501Y, A570D, D614G, P681H, T716I, S982A, D1118H H69-, Y144-, H245-, S247-, V70-, L244-, R246-, Y248- |
| 8 | Leźno, Pomorskie | B.1.617.2 | T19R, T95I, G142D, R158G, L452R, T478K, D614G, P681R, D950N, E156-, F157- |
| 9 | Kłoczew, Lubelskie | AY.122 | T19R, G142D, R158G, L452R, T478K, D614G, P681R, D950N E156-, F157- |
| 10 | Stefanów, Łódzkie | BA.2 | T19I, A27S, G142D, V213G, G339D, S371F, S373P, S375F, T376A, D405N, R408S, K417N, N440K, S477N, T478K, E484A, Q493R, Q498R, N501Y, Y505H, D614G, H655Y, N679K, P681H, N764K, D796Y, Q954H, N969K L24-, P25-, P26- |
Results of oral swab and serum sample analysis collected in two houses in Wisznice in the Lubelskie voivodeship
| House | Date of sample collection | Sample | Number | Results* | Prevalence (%) | |
|---|---|---|---|---|---|---|
| Collected | Positive | |||||
| 16/06/2021 | 20 | 2 | 21.8–25.5 | 10 | ||
| 1 | 19/07/2021 | Oral swab | 90 | 1 | 28.8 | 1.1 |
| 02/08/2021 | Serum of kitten | 15 | 15 | 1.25 | 100 | |
| Serum of adult | 15 | 14 | 1.64 | 93.3 | ||
| 16/06/2021 | 20 | 2 | 28.5–30.7 | 10 | ||
| 19/07/2021 | Oral swab | 60 | 0 | - | 0 | |
| 2 | Serum of kitten | 15 | 15 | 1.04 | 100 | |
| 02/08/2021 | Serum of adult | 15 | 14 | 1.25 | 93.3 | |