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Vaccine-induced rabies in a red fox in Poland Cover

Vaccine-induced rabies in a red fox in Poland

Journal of Veterinary Research
Volume 66 (2022): Issue 4 (December 2022)
By: Marcin Smreczak,  Anna Orłowska,  Thomas Müller,  Conrad M. Freuling,  Magdalena Kawiak-Sadurska and  Paweł Trębas  
Open Access
|Nov 2022

Figures & Tables

Fig. 1

PCR-RFLP results using Dra I (A), Mbo II (B), Msp I (C) and Nla IV (D) restriction endonucleases. M – DNA molecular mass ruler; 1 – vaccine-induced rabies, fox; 2 – rabies vaccine strain Street Alabama Duferrin Bern; 3 – rabies virus street/field strain
PCR-RFLP results using Dra I (A), Mbo II (B), Msp I (C) and Nla IV (D) restriction endonucleases. M – DNA molecular mass ruler; 1 – vaccine-induced rabies, fox; 2 – rabies vaccine strain Street Alabama Duferrin Bern; 3 – rabies virus street/field strain

Fig. 2

Phylogenetic relationship of the vaccine-induced rabies virus isolated from a fox in Poland (indicated in the blue box) with reference rabies virus (RABV) isolates available in GenBank based on complete genomes. The phylogenetic tree was generated using the neighbour-joining method (Kimura2 parameter) in MEGA5 software. Bootstrap values (1,000 replicates) over 70% indicating significant support for the tree topology are shown next to the branches
Phylogenetic relationship of the vaccine-induced rabies virus isolated from a fox in Poland (indicated in the blue box) with reference rabies virus (RABV) isolates available in GenBank based on complete genomes. The phylogenetic tree was generated using the neighbour-joining method (Kimura2 parameter) in MEGA5 software. Bootstrap values (1,000 replicates) over 70% indicating significant support for the tree topology are shown next to the branches

Fig. 3

Section of canine teeth showing yellow fluorescent lines of tetracycline
Section of canine teeth showing yellow fluorescent lines of tetracycline

Distribution of viral RNA in particular predefined organs

OrganThreshold cycleCopies/Reaction
Brain19.379.01e+005
Spinal cord23.037.25e +004
Salivary glands36.795.62
Tongue26.626.14e+003
DOI: https://doi.org/10.2478/jvetres-2022-0065 | Journal eISSN: 2450-8608
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Language: English
Page range: 473 - 477
Submitted on: Jun 3, 2022
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Accepted on: Nov 3, 2022
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Published on: Nov 15, 2022
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
fox,
rabies,
SAD Bern,
vaccine-induced rabies,
sequencing
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2022 Marcin Smreczak, Anna Orłowska, Thomas Müller, Conrad M. Freuling, Magdalena Kawiak-Sadurska, Paweł Trębas, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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