Have a personal or library account? Click to login
Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella abortus in organs of infected cattle in southern Italy Cover

Evaluation of a real-time PCR in combination with a cultivation method for the detection of Brucella abortus in organs of infected cattle in southern Italy

Journal of Veterinary Research
Volume 66 (2022): Issue 4 (December 2022)
By: Viviana Manzulli,  Valeria Rondinone,  Luigina Serrecchia,  Antonio Petrella,  Domenico Scaltrito,  Leonardo Marino,  Lorenzo Pace,  Maria Luigia Prencipe,  Dora Cipolletta,  Mauro Nitti,  Antonio Fasanella,  Elena Poppa and  Domenico Galante  
Open Access
|Dec 2022

Figures & Tables

Fig. 1

Real-time PCR analysis performed on DNA extracted from organs cultivated in the Brucella enrichment broth after 1 week of incubation
Real-time PCR analysis performed on DNA extracted from organs cultivated in the Brucella enrichment broth after 1 week of incubation

Results of serological, culture and real-time PCR analyses in organs of slaughtered cattle following a brucellosis outbreak

AnimalSerological statusLymph node
UdderSpleenCotyledon
Submandibular
Supramammary
Retropharyngeal
Internal iliac
PCRCulturePCRCulturePCRCulturePCRCulturePCRCulturePCRCulturePCRCulture
ARBT+ / CFT++ (•)+ (•••)−−+ (•)+ (••)+ (••)+ (•••)−−+ (••)+ (•••)Notsampled
BRBT+ / CFT++ (•)+ (•••)−−−−+ (hom)+ (••)−−+ (•)+ (•••)Notsampled
CRBT+ / CFT++ (•)+ (••)−−+ (•)+ (••)+ (•)+ (••)+ (•)+ (•••)+ (•)+ (••)+ (•)+ (••)
DRBT+ / CFT++ (•)+ (•••)−−+ (•)+ (••)+ (•)+ (••)−−−−+ (•)+ (••)
ERBT+ / CFT++ (•)+ (••)+ (•)+ (••)−−+ (•)+ (••)−−−−+ (•)+ (••)
FRBT+ / CFT++ (•)+ (•)+ (•)+ (••)+ (•)+ (••)+ (•)+ (•)+ (•)+ (••)+ (•)+ (••)+ (hom)+ (•)
GRBT+ / CFT++ (•)+ (••)−−+ (•)+ (••)+ (•)+ (••)−−+ (•)+ (••)Notsampled
HRBT+ / CFT−+ (•)+ (••)+ (•)+ (••)+ (•)+ (••)+ (•)+ (••)−−−−−−
IRBT− / CFT−+ (••)+ (•••)−−+ (•)+ (••)+ (•)+ (••)−−+ (•)+ (••)+ (hom)+ (•)
LRBT− / CFT−+ (•)+ (••)−−+ (hom)+ (••)−−−−−−+ (•)+ (••)
DOI: https://doi.org/10.2478/jvetres-2022-0062 | Journal eISSN: 2450-8608
Journal RSS Feed
Language: English
Page range: 559 - 563
Submitted on: May 18, 2022
|
Accepted on: Oct 26, 2022
|
Published on: Dec 18, 2022
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
diagnosis,
real-time PCR
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2022 Viviana Manzulli, Valeria Rondinone, Luigina Serrecchia, Antonio Petrella, Domenico Scaltrito, Leonardo Marino, Lorenzo Pace, Maria Luigia Prencipe, Dora Cipolletta, Mauro Nitti, Antonio Fasanella, Elena Poppa, Domenico Galante, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Previous articleVolume 66 (2022): Issue 4 (December 2022)Next article