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Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.) Cover

Genotoxic effect of cadmium and zinc in the peripheral erythrocytes of Prussian carp (Carassius gibelio B.)

Journal of Veterinary Research
Volume 66 (2022): Issue 4 (December 2022)
By: Ewa Drąg-Kozak,  Marta Kuchta-Gładysz,  Anna Grzesiakowska,  Ewa Łuszczek-Trojnar and  Magdalena Socha  
Open Access
|Nov 2022

Figures & Tables

Fig. 1

Erythrocytes of Prussian carp with different levels of DNA damage in the comet assay: cells from control samples after 14 days (a), after 21 days (c), and after 28 days (e); cells after exposure to Cd for 14 days (b), for 21 days (d) and for 28 days (f). 400×
Erythrocytes of Prussian carp with different levels of DNA damage in the comet assay: cells from control samples after 14 days (a), after 21 days (c), and after 28 days (e); cells after exposure to Cd for 14 days (b), for 21 days (d) and for 28 days (f). 400×

Fig. 2

Giemsa-stained peripheral blood smear of erythrocytes of Prussian carp with micronuclei (a and b), a nuclear bud (c), a notched nucleus (d), binucleated cells with unequal nuclear division (e), and a cell with abnormal cytoplasm (f). Arrows show the abnormalities. Erythrocytes are from the control group after 21 days of the experiment (c and d), exposed to Cd after 14 days (f) and exposed to Cd after 28 days (a, b and e). 1000×
Giemsa-stained peripheral blood smear of erythrocytes of Prussian carp with micronuclei (a and b), a nuclear bud (c), a notched nucleus (d), binucleated cells with unequal nuclear division (e), and a cell with abnormal cytoplasm (f). Arrows show the abnormalities. Erythrocytes are from the control group after 21 days of the experiment (c and d), exposed to Cd after 14 days (f) and exposed to Cd after 28 days (a, b and e). 1000×

Comet assay results for Prussian carp exposed to different treatments of cadmium, zinc and their binary mixture

Group
ControlCdZnCd + Zn
DayDNA in Tail (%)
1426.30 ± 0.56 Aa31.78 ± 0.69 Ab33.35 ± 0.67 Ab37.87 ± 0.76 Ac
2128.08 ± 0.65 Aa31.84 ± 0.59 Ab24.40 ± 0.53 Bc31.89 ± 0.64 Bb
2832.04 ± 0.61 Ba32.87 ± 0.67 Aa28.58 ± 0.51 Cb31.90 ± 0.59 Ba

The frequency of erythrocyte nuclear and cellular abnormalities in Prussian carp exposed to different concentrations of cadmium, zinc and their binary mixture

Group
ControlCdZnCd + Zn
DayMN (one or more micronuclei)
142.30 ± 0.36 Aa12.49 ± 1.08 Ab7.35 ± 0.80 Ac16.07 ± 1.68 Ab
211.29 ± 0.21 Ba4.00 ± 0.48 Bb4.61 ± 1.14 ABb3.67 ± 0.43 Bb
283.11 ± 0.34 Aab2.33 ± 0.298 Ca3.69 ± 0.34 Bbc4.53 ± 0.63 Bc
DOI: https://doi.org/10.2478/jvetres-2022-0057 | Journal eISSN: 2450-8608
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Language: English
Page range: 619 - 628
Submitted on: Apr 11, 2022
Accepted on: Oct 12, 2022
Published on: Nov 4, 2022
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
genotoxicity,
heavy metals,
erythrocyte micronucleus assay,
comet assay,
Prussian carp
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2022 Ewa Drąg-Kozak, Marta Kuchta-Gładysz, Anna Grzesiakowska, Ewa Łuszczek-Trojnar, Magdalena Socha, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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