Fig. 1
Fig. 2
Significantly differentially expressed proteins (P ≤ 0_05) in diabetic dogs identified by matrix-assisted laser desorption/ionisation–tandem time-of-flight mass spectrometry
| ID | Protein | Accession number (UniProtKB) | Score | Match | MW (kDa)* | pI* | Modif. | Seq. cov. (%) | Rt** C : D | Rt** C : D ret. | P-value |
|---|---|---|---|---|---|---|---|---|---|---|---|
| 1 | Ras-Rab-related 13 protein | F1PTE3 | 79 | 6 | 22881 | 9.36 | Carb, (M) Ox | 24 | 0.655 | 0.455 | 0.048 |
| 2 | Aldo-family keto 1 member reductase C3 | Q5R7C9 | 70 | 9 | 37224 | 7.13 | Carb | 18 | 0.793 | 0.015 | 0.015 |
| 3 | 28S ribosomal protein S31, mitochondrial | B0BN56 | 111 | 8 | 44220 | 8.46 | Carb, Ox (M) | 14 | 0.189 | 0.502 | 0.032 |
| 4 | 2synthase ′-5′-oligoadenylate 3 | Q8VI93 | 120 | 11 | 127907 | 8.99 | Carb, (M) Ox | 8 | 2.709 | 1.741 | 0.030 |
| 5 | 60protein S ribosomal L5 | P46777 | 81 | 20 | 34568 | 9.78 | Carb, (M) Ox | 42 | 0.074 | 0.128 | 0.001 |
Clinical characteristics of the study subjects
| Variable | Healthy subject n = 12 | With diabetic retinopathy n = 8 | Diabetic without retinopathy n = 12 |
|---|---|---|---|
| Age, Y | |||
| Mean (±SD) | 10.8 ± 1.8 | 10.3 ± 2.8 | 11.4 ± 2.1 |
| Range | 8–13 | 7–16 | 8–12 |
| Sex | |||
| Male | 7 | 3 | 4 |
| Female | 5 | 5 | 8 |
| Weight, kg | |||
| Mean (±SD) | 23.1 ± 7.7 | 13.9 ± 8.6 | 14.4 ± 5.3 |
| Range | 10–37 | 5.4–38 | 6.3–21 |
| Duration of diabetes, y | |||
| Mean (±SD) | 0 | 1.2 ± 0.6 | 2.4 ± 0.2 |
| Duration | 0 | 0.5–2 | 1–4 |
| Blood glucose, mmol/L | |||
| Mean (±SD) | 5.9 ± 0.2 | 17.8 ± 6 | 18.3 ± 2.3 |
| Range | 5.6–6.2 | 8.3–29.7 | 9.3–22.3 |
| Cataract | 0 | 0 | 0 |