Fig. 1
Blood stage of Plasmodium spp. found in fighting cocks in Maha Sarakham, Thailand
A – trophozoites of P. juxtanucleare; B – meronts of P. juxtanucleare; C – meronts of P. gallinaceum. Arrows indicate the parasite in each case
Fig. 2
Phylogenetic tree based on partial cytochrome Plasmodium and Leucocytozoon b gene sequences from Maha Sarakham (indicated in bold typeface) together with 23 related sequences from different distributions in Thailand and neighbouring countries in the GenBank and MalAvi databases. Sequences were compared with the maximum likelihood method. The cytochrome b gene of Leucocytozoon caulleryi from this study and related sequences in the database were used to root the tree. The percentage of trees in which associated taxa clustered together is shown next to the branches
Table 1
Haplotypes of 61 cytochrome b sequences from fighting cocks in Thailand and National Center for Biotechnology Information BLAST results
| Haplotype number (n) | Sample IDs | (GenBank accession number) | Closest sequences in NCBI GenBank (% similarity) |
|---|---|---|---|
| CAN1 (21) | BO3, BO13, BO21, CC10, CY2, CY17, CY18, CY24, KA1, KA13, KA20, KA30, KO7, KO19, ND17, PP5, PP10, PP16, WP16, WP20, WP22 | MW600907 | Plasmodium juxtanucleare MG598396.1 (99–100%) MGMG598393.1 598392.1 ((9999––100100%) %) |
| CAN2 (1) | BO4 | MW600908 | Plasmodium KU248845.1 juxtanucleare (99.8%) |
| CAN3 (3) | BO6, CC17, CC28 | MW600909 | Plasmodium MG598396.1 juxtanucleare (99–100%) |
| CAN4 (19) | BO15, BO23, CC9, CC18, CY10, KA2, KA12, KO11, KR3, KR7, KR19, KR21, KR23, ND11, PP1, PP3, PP8, WP6, WP28 | MW600910 | Plasmodium juxtanucleare MG598396.1 (99–100%) MG598393.1 (99–100%) MG598392.1 (99–100%) |
| CAN5 (3) | CC1, KA29, ND8 | MW600911 | Plasmodium juxtanucleare KU248845.1 (99–100%) AB250415.1 (99–100%) |
| CAN6 (3) | CC2, KO18, KR5 | MW600912 | Plasmodium juxtanucleare KU248845.1 (99–100%) AB250415.1 (99–100%) |
| CAN7 (1) | CY1 | MW600913 | Plasmodium MG598396.1 juxtanucleare (99.4%) |
| CAN8 (1) | CY9 | MW600914 | Plasmodium MG598396.1 juxtanucleare (99%) |
| CAN9 (1) | KO2 | MW600906 | Plasmodium gallinaceum LCLN506179.1 835294.1 (99(99%) %) KP025675.1 (99%) |
| CAN10 (3) | KO21, KO23, WP10 | MW600915 | Plasmodium MG598396.1 juxtanucleare (99–100%) |
| CAN11 (1) | KR1 | MW600918 | Leucocytozoon LC550057.1 (99.8caulleryi %) |
| CA12 (2) | ND5, ND20 | MW600916 | Plasmodium juxtanucleare MG598396.1 (99–100%) |
| CAN13 (1) | ND14 | MW600917 | Plasmodium KU248845.1 juxtanucleare (100%) |
| CAN14 (1) | PP13 | MW600919 | Leucocytozoon caulleryi LC550057.1 (99.8%) KT290932.1 (99.6%) |