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High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method Cover

High prevalence of Plasmodium infection in fighting cocks in Thailand determined with a molecular method

Open Access
|Sep 2022

Figures & Tables

Fig. 1

Blood stage of Plasmodium spp. found in fighting cocks in Maha Sarakham, Thailand
A – trophozoites of P. juxtanucleare; B – meronts of P. juxtanucleare; C – meronts of P. gallinaceum. Arrows indicate the parasite in each case
Blood stage of Plasmodium spp. found in fighting cocks in Maha Sarakham, Thailand A – trophozoites of P. juxtanucleare; B – meronts of P. juxtanucleare; C – meronts of P. gallinaceum. Arrows indicate the parasite in each case

Fig. 2

Phylogenetic tree based on partial cytochrome Plasmodium and Leucocytozoon b gene sequences from Maha Sarakham (indicated in bold typeface) together with 23 related sequences from different distributions in Thailand and neighbouring countries in the GenBank and MalAvi databases. Sequences were compared with the maximum likelihood method. The cytochrome b gene of Leucocytozoon caulleryi from this study and related sequences in the database were used to root the tree. The percentage of trees in which associated taxa clustered together is shown next to the branches
Phylogenetic tree based on partial cytochrome Plasmodium and Leucocytozoon b gene sequences from Maha Sarakham (indicated in bold typeface) together with 23 related sequences from different distributions in Thailand and neighbouring countries in the GenBank and MalAvi databases. Sequences were compared with the maximum likelihood method. The cytochrome b gene of Leucocytozoon caulleryi from this study and related sequences in the database were used to root the tree. The percentage of trees in which associated taxa clustered together is shown next to the branches

Haplotypes of 61 cytochrome b sequences from fighting cocks in Thailand and National Center for Biotechnology Information BLAST results

Haplotype number (n)Sample IDs(GenBank accession number)Closest sequences in NCBI GenBank (% similarity)
CAN1 (21)BO3, BO13, BO21, CC10, CY2, CY17, CY18, CY24, KA1, KA13, KA20, KA30, KO7, KO19, ND17, PP5, PP10, PP16, WP16, WP20, WP22MW600907Plasmodium juxtanucleare MG598396.1 (99–100%) MGMG598393.1 598392.1 ((9999––100100%) %)
CAN2 (1)BO4MW600908Plasmodium KU248845.1 juxtanucleare (99.8%)
CAN3 (3)BO6, CC17, CC28MW600909Plasmodium MG598396.1 juxtanucleare (99–100%)
CAN4 (19)BO15, BO23, CC9, CC18, CY10, KA2, KA12, KO11, KR3, KR7, KR19, KR21, KR23, ND11, PP1, PP3, PP8, WP6, WP28MW600910Plasmodium juxtanucleare MG598396.1 (99–100%) MG598393.1 (99–100%) MG598392.1 (99–100%)
CAN5 (3)CC1, KA29, ND8MW600911Plasmodium juxtanucleare KU248845.1 (99–100%) AB250415.1 (99–100%)
CAN6 (3)CC2, KO18, KR5MW600912Plasmodium juxtanucleare KU248845.1 (99–100%) AB250415.1 (99–100%)
CAN7 (1)CY1MW600913Plasmodium MG598396.1 juxtanucleare (99.4%)
CAN8 (1)CY9MW600914Plasmodium MG598396.1 juxtanucleare (99%)
CAN9 (1)KO2MW600906Plasmodium gallinaceum LCLN506179.1 835294.1 (99(99%) %) KP025675.1 (99%)
CAN10 (3)KO21, KO23, WP10MW600915Plasmodium MG598396.1 juxtanucleare (99–100%)
CAN11 (1)KR1MW600918Leucocytozoon LC550057.1 (99.8caulleryi %)
CA12 (2)ND5, ND20MW600916Plasmodium juxtanucleare MG598396.1 (99–100%)
CAN13 (1)ND14MW600917Plasmodium KU248845.1 juxtanucleare (100%)
CAN14 (1)PP13MW600919Leucocytozoon caulleryi LC550057.1 (99.8%) KT290932.1 (99.6%)
Language: English
Page range: 373 - 379
Submitted on: Apr 22, 2022
Accepted on: Sep 2, 2022
Published on: Sep 15, 2022
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year

© 2022 Kotchaphon Vaisusuk, Wasupon Chatan, Tossapol Seerintra, Supawadee Piratae, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.