Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China Cover

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Molecular characteristics and genetic diversity of Fasciola hepatica from sheep in Xinjiang, China

Journal of Veterinary Research

Volume 66 (2022): Issue 2 (June 2022)

By: Wang Xifeng, Zhang Kai, Zhang Guowu, Li Zhiyuan, Shang Yunxia, Ning Chengcheng, Ji Chunhui, Qiao Jun, Meng Qingling and Cai Xuepeng

Open Access

|Apr 2022

Figures & Tables

Morphological identification of parasites from sheep. A – parasites found in bile ducts of sheep; B – parasites fixed on slides with 75% alcohol after washing with phosphate buffered saline (0.01M, pH 7.2–7.4)

Median-joining network of F. hepatica isolates based on cox1 (A) and nad1 (B) genes’ haplotypes. The size of the circle indicates the frequency of the haplotype. The colour of the circle indicates the origin of the population. The green dot represents the default vertex

Phylogenetic tree of haplotypes of different geographic populations of F. hepatica in Xinjiang. The tree is based on cox1 (A) and nad1 (B) and constructed with the neighbour-joining method

Mismatch distribution of haplotypes of the cox1 (A) and nad1 (B) genes. The number of nucleotide differences between pairs of sequences is indicated along the x–axis, and their frequency along the y–axis. Obs – observed value; Exp – expected value

Molecular variation analysis based on cox1 and nad1 gene sequences

Source of variation	Gene	Degrees of freedom	Sum of squares	Variance components	Percentage of variation (%)
Within	cox1	141	62.933	0.44634 Vb	99.70
populations	nad1	141	55.711	0.39511 Vb	96.48
Among	cox1	2	0.990	0.00133 Va	0.30
populations	nad1	2	1.844	0.01442 Va	3.52
Total	cox1	143	63.924	0.44767
Total	nad1	143	57.556	0.40953
F–statistic	cox1		0.04451, P > 0.05
F–statistic	nad1		0.03520, P > 0.05

Genetic diversity parameters of the three geographic populations of F_ hepatica

Genes	Populations	N	h	Hd	K	π
	Urumqi	45	18	0.909 ± 0.00089	3.160	0.00637
cox1	Hami	90	23	0.890 ± 0.00047	3.280	0.00661
	Kuqa	9	6	0.833 ± 0.01600	2.722	0.00549
	Total	144	34	0.894 ± 0.00034	3.287	0.00663
	Urumqi	45	12	0.835 ± 0.00118	2.638	0.00400
nad1	Hami	90	25	0.7590 ± 0.00218	2.778	0.00421
	Kuqa	9	6	0.889 ± 0.00828	2.583	0.00391
	Total	144	33	0.805 ± 0.00086	2.969	0.00450

Neutrality indices based on cox1 and nad1 genes of F_ hepatica in different geographical regions of Xinjiang

Population	cox1		nad1

	Tajima’s D	Fu’s Fs	Tajima’s D	Fu’s Fs
Urumqi	–0.74410	–7.514	–0.72079	–2.547
Hami	–0.36276	–8.659	–1.94511*	–13.448
Kuqa	–0.34120	–1.395	–0.55536	–1.522
Total	–1.01276	–19.204	–1.92702*	–19.881

DOI: https://doi.org/10.2478/jvetres-2022-0018 | Journal eISSN: 2450-8608

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Language: English

Page range: 199 - 207

Submitted on: Sep 14, 2021

Accepted on: Mar 18, 2022

Published on: Apr 22, 2022

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Publication frequency: 4 issues per year

Keywords:

genetic diversity,

population structure

Related subjects:

Molecular biology,

Microbiology and virology,

Life sciences, other,

Veterinary medicine

© 2022 Wang Xifeng, Zhang Kai, Zhang Guowu, Li Zhiyuan, Shang Yunxia, Ning Chengcheng, Ji Chunhui, Qiao Jun, Meng Qingling, Cai Xuepeng, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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