Antibiotic susceptibility of mycobacteria isolated from ornamental fish

S	I	R	S	I	R
AMK	1–128	≤16	32	≥64	-	-	>32
KAN	1–64	≤16	32	≥64	-	-	>32
TOB	1–64	≤1	2–4	≥8	-	-	>4
DOX	0.25–32	≤2	4	≥8	≤2	4	≥8
CLR	0.25–32	≤2	4	≥8	≤8	16	≥32
CIP	0.125–16	≤1	2	≥4	-	-	>2
SMX	1–64	≤32	-	≥64	≤32	-	≥64
RMP	0.25–64	-	-	>1	-	-	>1
INH	1–64	-	-	≥5	-	-	≥5

[i] MIC – minimum inhibitory concentration; RGM – rapidly growing mycobacteria; SGM – slowly growing mycobacteria; S – susceptible; I – intermediate; R – resistant; AMK – amikacin; KAN – kanamycin; TOB – tobramycin; DOX – doxycycline; CLR – clarithromycin; CIP – ciprofloxacin; SMX – sulfamethoxazole; RMP – rifampicin; INH – isoniazid

Table 2

Antimicrobial resistance or susceptibility determined by microdilution

NTM species (number of isolates tested)	Resistant (R) or susceptible (S)	Percentage of strains resistant or susceptible for each antimicrobial agent
NTM species (number of isolates tested)	Resistant (R) or susceptible (S)	Amikacin	Kanamycin	Tobramycin	Doxycycline	Clarithromycin	Ciprofloxacin	Sulfamethoxazole	Rifampicin	Isoniazid
M. abscessus (n = 1)	R			100.00	100.00			100.00	100.00	100.00
	I						100.00
	S	100.00	100.00			100.00
M. chelonae (n = 16)	R	6.25		43.75	100.00	6.25	100.00	100.00	100.00	100.00
	I	12.50	12.50	50.00
	S	81.25	87.50	6.25		93.75
M. fortuitum (n = 10)	R	10.00		100.00	90.00	50.00	10.00	10.00	100.00	100.00
	I		10.00			40.00
	S	90.00	90.00		10.00	10.00	90.00	90.00
M. (n gordonae = 15)	R			20.00		6.67	20.00		93.33	100.00
	I			46.67
	S	100.00	100.00	33.33	100.00	93.33	80.00	100.00	6.67
M. marinum (n = 33)	R			3.03	9.09		12.12		93.94	100.00
	I			87.88		3.03
	S	100.00	100.00	9.09	90.91	96.97	87.88	100.00	6.06
M. (n mucogenicum = 1)	R			100.00	100.00		100.00		100.00	100.00
	I					100.00
	S	100.00	100.00					100.00
M. neoaurum (n = 2)	R								100.00	100.00
	I
	S	100.00	100.00	100.00	100.00	100.00	100.00	100.00
M. peregrinum (n = 12)	R	8.33	8.33	8.33	66.67	8.33	8.33		100.00	100.00
	I			33.33	8.33	8.33
	S	91.67	91.67	58.33	25.00	83.34	91.67	100.00
M. salmoniphilum (n = 1)	R				100.00				100.00	100.00
	I
	S	100.00	100.00	100.00		100.00	100.00	100.00
M. saopaulense (n = 1)	R				100.00			100.00	100.00	100.00
	I			100.00			100.00
	S	100.00	100.00			100.00
M. senegalense (n = 4)	R				25.00	25.00	75.00	25.00	100.00	100.00
	I			25.00			25.00
	S	100.00	100.00	75.00	75.00	75.00		75.00
M. septicum (n = 2)	R				50.00				100.00	100.00
	I				50.00		50.00
	S	100.00	100.00	100.00		100.00	50.00	100.00
M. szulgai (n = 1)	R			100.00			100.00		100.00
	I
	S	100.00	100.00		100.00	100.00		100.00		100.00

[i] NTM – nontuberculous mycobacteria; S – susceptible; I – intermediate; R – resistant

Table 3

Antibiotic susceptibility profiles of RGM and SGM isolated from diseased fish

S	I	R	S	I	R
AMK	90.00	4.00	6.00	100	0.00	0.00
KAN	92.00	6.00	2.00	100	0.00	0.00
TOB	32.00	28.00	40.00	16.33	73.47	10.20
DOX	18.00	4.00	78.00	93.88	0.00	6.12
CLR	72.00	12.00	16.00	95.92	2.04	2.04
CIP	48.00	8.00	44.00	83.67	0.00	16.33
SMX	60.00	0.00	40.00	100.00	0.00	0.00
RMP	0.00	0.00	100.00	6.12	0.00	93.88
INH	0.00	0.00	100.00	2.04	0.00	97.96

[i] RGM – rapidly growing mycobacteria; SGM – slowly growing mycobacteria; S – susceptible; I – intermediate; R – resistant; AMK – amikacin; KAN – kanamycin; TOB – tobramycin; DOX – doxycycline; CLR – clarithromycin; CIP – ciprofloxacin; SMX – sulfamethoxazole; RMP – rifampicin; INH – isoniazid

References

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Articles in this issue

DOI: https://doi.org/10.2478/jvetres-2022-0011 | Journal eISSN: 2450-8608 (formerly 2300-3235)

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Language: English

Page range: 69 - 76

Submitted on: Sep 3, 2021

Accepted on: Mar 1, 2022

Published on: Mar 25, 2022

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

Mycobacterium spp,

antimicrobial susceptibility,

minimum inhibitory concentration,

fish

Related subjects:

Life sciences,

Molecular biology,

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2022 Leszek Guz, Krzysztof Puk, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 66 (2022): Issue 1 (March 2022)