Antibiotic susceptibility of mycobacteria isolated from ornamental fish

Guz, Leszek; Puk, Krzysztof

doi:10.2478/jvetres-2022-0011

Figures & Tables

Antimicrobial resistance or susceptibility determined by microdilution

NTM species (number of isolates tested)	Resistant (R) or susceptible (S)	Percentage of strains resistant or susceptible for each antimicrobial agent
NTM species (number of isolates tested)	Resistant (R) or susceptible (S)	Amikacin	Kanamycin	Tobramycin	Doxycycline	Clarithromycin	Ciprofloxacin	Sulfamethoxazole	Rifampicin	Isoniazid
M. abscessus (n = 1)	R			100.00	100.00			100.00	100.00	100.00
	I						100.00
	S	100.00	100.00			100.00
M. chelonae (n = 16)	R	6.25		43.75	100.00	6.25	100.00	100.00	100.00	100.00
	I	12.50	12.50	50.00
	S	81.25	87.50	6.25		93.75
M. fortuitum (n = 10)	R	10.00		100.00	90.00	50.00	10.00	10.00	100.00	100.00
	I		10.00			40.00
	S	90.00	90.00		10.00	10.00	90.00	90.00
M. (n gordonae = 15)	R			20.00		6.67	20.00		93.33	100.00
	I			46.67
	S	100.00	100.00	33.33	100.00	93.33	80.00	100.00	6.67
M. marinum (n = 33)	R			3.03	9.09		12.12		93.94	100.00
	I			87.88		3.03
	S	100.00	100.00	9.09	90.91	96.97	87.88	100.00	6.06
M. (n mucogenicum = 1)	R			100.00	100.00		100.00		100.00	100.00
	I					100.00
	S	100.00	100.00					100.00
M. neoaurum (n = 2)	R								100.00	100.00
	I
	S	100.00	100.00	100.00	100.00	100.00	100.00	100.00
M. peregrinum (n = 12)	R	8.33	8.33	8.33	66.67	8.33	8.33		100.00	100.00
	I			33.33	8.33	8.33
	S	91.67	91.67	58.33	25.00	83.34	91.67	100.00
M. salmoniphilum (n = 1)	R				100.00				100.00	100.00
	I
	S	100.00	100.00	100.00		100.00	100.00	100.00
M. saopaulense (n = 1)	R				100.00			100.00	100.00	100.00
	I			100.00			100.00
	S	100.00	100.00			100.00
M. senegalense (n = 4)	R				25.00	25.00	75.00	25.00	100.00	100.00
	I			25.00			25.00
	S	100.00	100.00	75.00	75.00	75.00		75.00
M. septicum (n = 2)	R				50.00				100.00	100.00
	I				50.00		50.00
	S	100.00	100.00	100.00		100.00	50.00	100.00
M. szulgai (n = 1)	R			100.00			100.00		100.00
	I
	S	100.00	100.00		100.00	100.00		100.00		100.00

Antibiotic susceptibility profiles of RGM and SGM isolated from diseased fish

Antimicrobial agent	Susceptibility
	RGM (n = 50)			SGM (n = 49)
	S	I	R	S	I	R
AMK	90.00	4.00	6.00	100	0.00	0.00
KAN	92.00	6.00	2.00	100	0.00	0.00
TOB	32.00	28.00	40.00	16.33	73.47	10.20
DOX	18.00	4.00	78.00	93.88	0.00	6.12
CLR	72.00	12.00	16.00	95.92	2.04	2.04
CIP	48.00	8.00	44.00	83.67	0.00	16.33
SMX	60.00	0.00	40.00	100.00	0.00	0.00
RMP	0.00	0.00	100.00	6.12	0.00	93.88
INH	0.00	0.00	100.00	2.04	0.00	97.96

MIC breakpoints used for categorisation of susceptibility of RGM and SGM to nine antimicrobial agents

Antimicrobial agent	Range (μg/mL)	MIC breakpoints (μg/mL)
		RGM			SGM
		S	I	R	S	I	R
AMK	1–128	≤16	32	≥64	-	-	>32
KAN	1–64	≤16	32	≥64	-	-	>32
TOB	1–64	≤1	2–4	≥8	-	-	>4
DOX	0.25–32	≤2	4	≥8	≤2	4	≥8
CLR	0.25–32	≤2	4	≥8	≤8	16	≥32
CIP	0.125–16	≤1	2	≥4	-	-	>2
SMX	1–64	≤32	-	≥64	≤32	-	≥64
RMP	0.25–64	-	-	>1	-	-	>1
INH	1–64	-	-	≥5	-	-	≥5

Antibiotic susceptibility of mycobacteria isolated from ornamental fish

Figures & Tables

Antimicrobial resistance or susceptibility determined by microdilution

Antibiotic susceptibility profiles of RGM and SGM isolated from diseased fish

MIC breakpoints used for categorisation of susceptibility of RGM and SGM to nine antimicrobial agents

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