Field trial of antibody response to inactivated bacterial vaccine in young Holstein calves: influence of animal health status

(a)	Experiment	Antibody titre	BMI	Nutritional status	Oxidative stress	Positive	Negative
	First	✓	✓			77	30
H. somni	Second	✓	✓	✓		41	25
	Third	✓	✓	✓	✓	25	22
	First	✓	✓			101	6
P. multocida	Second	✓	✓	✓		61	5
	Third	✓	✓	✓	✓	42	5
	First	✓	✓			37	70
M. haemolytica	Second	✓	✓	✓		8	58
	Third	✓	✓	✓	✓	8	39
(b)		MAL	Positive		Negative		P value
		50	38		4
		100	45		1
P. multocida		200	15		1		-
		400	3		0
		50	3		16		0.058
		100	9		28		0.105
M. haemolytica**		200	12		19		0.566
		400	11		7		0.009**
		800	2		0		0.050*

[i] No significant interaction between MAL and the number of positive or negative calves was found for Pasteurella multocida (P = 0.421), but one was found for Mannheimia haemolytica (P < 0.01). H. somni – Histophilus somni; MAL – maternal antibody level; * –P < 0.05; ** –P < 0.01

BMI of the positive and negative group calves at the primary and booster immunisation in the first experiment. * – P < 0.05; ** – P < 0.01; # – P = 0.062

Table 2

The nutritional status in the second experiment (a) and oxidative stress marker values in the third experiment (b)

	Positive	Negative	Positive	Negative	Positive	Negative
Total cholesterol	P	93.6 ± 5.3	98.5 ± 5.1	96.1 ± 4.0	88.2 ± 13.4	79.3 ± 8.3	97.7 ± 4.1
(mg/dL)	B	118.3 ± 5.2	118.6 ± 4.6	118.7 ± 3.8	114.4 ± 12.8	113.8 ± 10.0	119.0 ± 3.9
Blood urea nitrogen	P	12.1 ± 0.7	10.9 ± 0.4	11.7 ± 0.5	10.4 ± 0.6	13.3 ± 2.3	11.4 ± 0.5
(mg/dL)	B	9.3 ± 0.3	10.4 ± 0.4	9.6 ± 0.2	11.4 ± 0.4	9.3 ± 0.6	9.8 ± 0.2
Total protein	P	5.8 ± 0.1*	5.6 ± 0.1	5.7 ± 0.1	5.5 ± 0.2	6.0 ± 0.2	5.7 ± 0.1
(g/dL)	B	6.0 ± 0.1	5.9 ± 0.1	6.0 ± 0.0	6.1 ± 0.2	6.0 ± 0.1	6.0 ± 0.0
Albumin/globulin	P	1.04 ± 0.03	1.08 ± 0.04	1.05 ± 0.03	1.11 ± 0.10	0.91 ± 0.08*	1.08 ± 0.02
ratio	B	1.15 ± 0.03	1.17 ± 0.03	1.16 ± 0.02	1.14 ± 0.08	1.07 ± 0.03	1.17 ± 0.02
Albumin	P	2.90 ± 0.04	2.87 ± 0.05	2.89 ± 0.03	2.87 ± 0.10	2.76 ± 0.08	2.91 ± 0.04
(g/dL)	B	3.19 ± 0.03	3.16 ± 0.04	3.18 ± 0.03	3.25 ± 0.09	3.11 ± 0.06	3.19 ± 0.03
Alpha globulin	P	1.06 ± 0.02	1.07 ± 0.03	1.06 ± 0.02	1.11 ± 0.07	1.15 ± 0.08	1.06 ± 0.02
(g/dL)	B	0.97 ± 0.02	0.99 ± 0.02	0.97 ± 0.02	1.04 ± 0.05	0.99 ± 0.03	0.97 ± 0.02
Beta globulin	P	1.00 ± 0.03	0.99 ± 0.03	1.00 ± 0.02	0.91 ± 0.06	1.05 ± 0.07	0.99 ± 0.02
(g/dL)	B	1.01 ± 0.02	1.01 ± 0.02	1.01 ± 0.01	1.04 ± 0.05	1.01 ± 0.05	1.01 ± 0.01
Gamma globulin	P	0.81 ± 0.05*	0.65 ± 0.06	0.76 ± 0.04	0.63 ± 0.12	0.98 ± 0.15*	0.72 ± 0.04
(g/dL)	B	0.83 ± 0.03*	0.75 ± 0.04	0.80 ± 0.03	0.79 ± 0.07	0.92 ± 0.09	0.78 ± 0.03

	Positive	Negative	Positive	Negative	Positive	Negative
d-ROMs	P	77.2 ± 2.8	83.4 ± 2.7	80.3 ± 2.2	78.4 ± 3.4	77.0 ± 5.7	80.8 ± 2.1
(Carr U)	B	76.0 ± 1.7	78.1 ± 2.5	76.7 ± 1.6	79.4 ± 5.1	74.8 ± 2.9	77.5 ± 1.7
BAP	P	2,934 ± 50	2,985 ± 52	2,959 ± 38	2,951 ±114	2,862 ± 83	2,978 ± 40
(μM)	B	2,843 ± 39	2,802 ± 52	2,825 ± 33	2,816 ± 122	2,933 ± 85	2,802 ± 33
	P	2.64 ± 0.10	2.81 ± 0.10	2.73 ± 0.08	2.66 ± 0.04	2.69 ± 0.19	2.73 ± 0.08
OSI	B	2.69 ± 0.08	2.82 ± 0.11	2.74 ± 0.07	2.85 ± 0.24	2.57 ± 0.15	2.79 ± 0.07

[i] H. somni – Histophilus somni; P. multocida – Pasteurella multocida; M. haemolytica – Mannheimia haemolytica; P – at the time of primary immunisation; B – at the time of booster immunisation; * – significant difference (P < 0.05) to the negative

[ii] H. somni – Histophilus somni; P. multocida – Pasteurella multocida; M. haemolytica – Mannheimia haemolytica; P – at the time of primary immunisation; B– at the time of booster immunisation; d-ROMs – diacron reactive oxygen metabolites; Carr U – Carratelli units; BAP – biological antioxidant potential; OSI – oxidative stress index

Table 3

Pearson’s correlation analysis

	H.	P.	M.	At	At	H.	P.	M.
	somni	multocida	haemolytica	primary	booster	somni	multocida	haemolytica
Maternal antibody titre	H. somni	-
	P. multocida	0.530**	-
	M. haemolytica	0.438**	0.492**	-
	At primary	0.182	0.048	0.198*	-
Body mass index	At booster	0.362**	0.298**	0.208*	0.446**	-
	H. somni	0.132	0.152	0.142	0.316**	0.383**	-
Post-immunisation	P. multocida	−0.033	0.132	0.085	0.370**	0.347**	0.452**	-
titre	M. haemolytica	0.061	0.227*	0.264**	0.389**	0.278**	0.357**	0.510**	-

H. somni	P. multocida	M. haemolytica
Total cholesterol	−0.171	−0.093	−0.145
Blood urea nitrogen	0.142	0.101	−0.043
Total protein	0.593**	0.539**	0.306*
Albumin/globulin ratio	−0.610**	−0.485**	−0.418**
Albumin	−0.068	−0.009	−0.119
Alpha globulin	0.003	0.092	0.000
Beta globulin	0.485**	0.303*	0.158
Gamma globulin	0.766**	0.683**	0.512**

[i] H. somni – Histophilus somni; P. multocida – Pasteurella multocida; M. haemolytica – Mannheimia haemolytica; Maternal antibody titre – the titre at the primary immunisation; Post-immunisation titre – the titre at three weeks after booster immunisation; * – P < 0.05; ** – P < 0.01

[ii] H. somni – Histophilus somni; P. multocida – Pasteurella multocida; M. haemolytica – Mannheimia haemolytica; Maternal antibody titre – the titre at the primary immunisation; * – P < 0.05; ** – P < 0.01

Relationship between body mass index at primary immunisation and maternal antibody titre against *Histophilus somni* at primary immunisation; post-immunisation titre and maternal antibody titre at primary immunisation against *H. somni*; post-immunisation titre against *H. somni* and body mass index at primary immunisation; and post-immunisation titre against *H. somni* and body mass index at booster vaccination in the first experiment (1) and between maternal antibody titre and serum total protein or gamma globulin at the primary immunisation in the second experiment (2). ** – P < 0.01; # – P < 0.10

References

Authors

Metrics

Articles in this issue

DOI: https://doi.org/10.2478/jvetres-2022-0003 | Journal eISSN: 2450-8608 (formerly 2300-3235)

Journal RSS Feed

Language: English

Page range: 109 - 116

Submitted on: Jul 4, 2021

Accepted on: Jan 11, 2022

Published on: Mar 25, 2022

Published by: National Veterinary Research Institute in Pulawy

In partnership with: Paradigm Publishing Services

Keywords:

early vaccination,

bovine respiratory disease,

Related subjects:

Microbiology and virology,

Life sciences, other,

Medicine,

Veterinary medicine

© 2022 Kazusa Mori, Konosuke Otomaru, Toshihide Kato, Osamu Yokota, Hiromichi Ohtsuka, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Volume 66 (2022): Issue 1 (March 2022)

Field trial of antibody response to inactivated bacterial vaccine in young Holstein calves: influence of animal health status

Figures & Tables

Table 1

Fig. 1

Table 2

Table 3

Fig. 2

Paradigm

My account