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Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation Cover

Mutations of p53 gene in canine sweat gland carcinomas probably associated with UV radiation

Journal of Veterinary Research
Volume 65 (2021): Issue 4 (December 2021)
By: Agnieszka Jasik,  Anna Kycko,  Monika Olech,  Krzysztof Wyrostek,  Anna Śmiech,  Wojciech Łopuszyński,  Iwona Otrocka-Domagała,  Mateusz Mikiewicz and  Izabella Dolka  
Open Access
|Dec 2021

Figures & Tables

Fig. 1

Example images of the laser capture microdissection of tumour cells acquired using Arcturus XT operating software (10× objective). A – Haematoxylin and eosin stained section, covered with xylene for visualisation, showing cystic/papillary type apocrine sweat gland carcinoma before microdissection, with the area containing tumour cells marked with a red line. B – The same section with marked tumour cells and laser infrared spots (IR spots). C – The same section after xylene evaporation and the microdissection of the marked cells. D – Tumour cells transferred to the macro cap after laser capture microdissection
Example images of the laser capture microdissection of tumour cells acquired using Arcturus XT operating software (10× objective). A – Haematoxylin and eosin stained section, covered with xylene for visualisation, showing cystic/papillary type apocrine sweat gland carcinoma before microdissection, with the area containing tumour cells marked with a red line. B – The same section with marked tumour cells and laser infrared spots (IR spots). C – The same section after xylene evaporation and the microdissection of the marked cells. D – Tumour cells transferred to the macro cap after laser capture microdissection

Fig. 2

Alignment of the amino acid sequence of exon 4 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein
Alignment of the amino acid sequence of exon 4 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein

Fig. 3

Alignment of the amino acid sequence of exon 5 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid references sequence of the p53 protein
Alignment of the amino acid sequence of exon 5 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid references sequence of the p53 protein

Fig. 4

Alignment of the amino acid sequence of exon 6 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinoma with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein
Alignment of the amino acid sequence of exon 6 of the p53 protein of the canine control (K1) sample and tested samples of apocrine sweat gland carcinoma with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein

Fig. 5

Alignment of the amino acid sequence of exon 8 of p53 of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein
Alignment of the amino acid sequence of exon 8 of p53 of the canine control (K1) sample and tested samples of apocrine sweat gland carcinomas with canine (U62133, AB020761) and human (K03199) amino acid reference sequences of the p53 protein

Mutations in the p53 gene in canine ASGC

Sample no.Histology*BreedAge (years)Body locationExonCodon †Mutation (amino acid)
180Papillary ASGC—5Chest (rib area)5157gTc → gCc (Val → Ala)
188Papillary ASGCGerman shepherd6Forelimb (arm area)8264aAc →a-c reading frame shift
190Solid ASGCMixed breed8Neck (side neck area)5178caC → caT (His = His)
191Ductal ASGCMixed breed10Chest (scapular area)5149, 150tcCCca → tcTTca (Ser = Ser; Pro > Ser)
193Solid ASGCDachshund9Forelimb (axillary area)5157gTc → gCc (Val → Ala)
gcT → gcC
63(Ala = Ala)
194Tubular ASGCGiant schnauzer—Finger4 6123aCt → aGt (Thr → Ser)
214aCt → aGt
(Thr → Ser)
201Ductal ASGCMixed breed12Head (auricle area)5157gTc → gCc (Val → Ala)
212Papillary ASGCMixed breed11—490tCc → tTc (Ser → Phe)
217Ductal ASGCMixed breed5—490tCc → tTc
(Ser → Phe)
219Cystic, ASGC papillaryMixed breed5Pelvis (gluteal area)490tCc → tTc (Ser → Phe)
220Solid ASGC—11Pelvis (anal area)490tCc → tTc (Ser → Phe)
221Solid ASGCFrench bulldog11—490tCc → tTc (Ser → Phe)
222Carcinosarcoma ASGMiniature schnauzer8Chest (rib area)490tCc → tTc (Ser → Phe)
DOI: https://doi.org/10.2478/jvetres-2021-0070 | Journal eISSN: 2450-8608
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Language: English
Page range: 519 - 526
Submitted on: Aug 13, 2021
Accepted on: Dec 9, 2021
Published on: Dec 20, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
dog,
apocrine sweat gland carcinoma,
gene, mutations,
UVR
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Agnieszka Jasik, Anna Kycko, Monika Olech, Krzysztof Wyrostek, Anna Śmiech, Wojciech Łopuszyński, Iwona Otrocka-Domagała, Mateusz Mikiewicz, Izabella Dolka, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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