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The markers of stress in swine oral fluid Cover

The markers of stress in swine oral fluid

Journal of Veterinary Research
Volume 65 (2021): Issue 4 (December 2021)
By:
Open Access
|Dec 2021

Figures & Tables

Fig. 1

The concentrations of cortisol (A) and cortisone (B) in oral fluid of swine. In the box plots, the boundary of the box closest to zero indicates the 25th percentile, the line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. The whiskers below and above the box indicate the minimum and maximum values, respectively. Results that differ statistically from the control are marked with an asterisk (*)
The concentrations of cortisol (A) and cortisone (B) in oral fluid of swine. In the box plots, the boundary of the box closest to zero indicates the 25th percentile, the line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. The whiskers below and above the box indicate the minimum and maximum values, respectively. Results that differ statistically from the control are marked with an asterisk (*)

Fig. 2

The concentration of cortisol (A) and cortisone (B) in swine plasma collected at 8 a.m. The results of evaluation of samples from individual animals are presented, together with the mean of all results for the stressed and control groups. Statistically significant difference between the control and treated groups is marked with an asterisk (*). The results are presented for the five pigs which were sampled every other day throughout the experiment. Two other animals were excluded from blood sampling on some days due to extensive swelling of the injection site
The concentration of cortisol (A) and cortisone (B) in swine plasma collected at 8 a.m. The results of evaluation of samples from individual animals are presented, together with the mean of all results for the stressed and control groups. Statistically significant difference between the control and treated groups is marked with an asterisk (*). The results are presented for the five pigs which were sampled every other day throughout the experiment. Two other animals were excluded from blood sampling on some days due to extensive swelling of the injection site

Fig. 3

Alpha-amylase activity in swine oral fluid expressed in milliunits (mU). Statistically significant difference between the control and experimental group is marked with an asterisk (*)
Alpha-amylase activity in swine oral fluid expressed in milliunits (mU). Statistically significant difference between the control and experimental group is marked with an asterisk (*)

Fig. 4

Total antioxidant capacity (TAC) in swine oral fluid, based on the trolox equivalent
Total antioxidant capacity (TAC) in swine oral fluid, based on the trolox equivalent

The parameters of the mass-spectrometry detection of corticoids and their respective internal standards

AnalytePrecursor ion (m/z)Fragment ion (m/z)Declustering potentialCollision energyIonisation
Prednisolone405295−60−45
405329−60−25
Cortisol407331−60−25
407297−60−45
Cortisone405329−60−20Negative mode
405301−60−30
Cortisol d4411335−60−60
Cortisone d8413337−60−24
Testosterone28910910035
2899710028
Testosterone d22919910031Positive mode
Corticosterone3471218032
Corticosterone3473118024
Corticosterone d43511218031
DOI: https://doi.org/10.2478/jvetres-2021-0065 | Journal eISSN: 2450-8608
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Language: English
Page range: 487 - 495
Submitted on: Jun 9, 2021
Accepted on: Nov 22, 2021
Published on: Dec 2, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
swine oral fluid,
acute stress markers,
oxidative stress,
cortisol,
alpha-amylase
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Marta Giergiel, Małgorzata Olejnik, Artur Jabłoński, Andrzej Posyniak, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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