Table 1
The parameters of the mass-spectrometry detection of corticoids and their respective internal standards
| Analyte | Precursor ion (m/z) | Fragment ion (m/z) | Declustering potential | Collision energy | Ionisation |
|---|---|---|---|---|---|
| Prednisolone | 405 | 295 | −60 | −45 | |
| 405 | 329 | −60 | −25 | ||
| Cortisol | 407 | 331 | −60 | −25 | |
| 407 | 297 | −60 | −45 | ||
| Cortisone | 405 | 329 | −60 | −20 | Negative mode |
| 405 | 301 | −60 | −30 | ||
| Cortisol d4 | 411 | 335 | −60 | −60 | |
| Cortisone d8 | 413 | 337 | −60 | −24 | |
| Testosterone | 289 | 109 | 100 | 35 | |
| 289 | 97 | 100 | 28 | ||
| Testosterone d2 | 291 | 99 | 100 | 31 | Positive mode |
| Corticosterone | 347 | 121 | 80 | 32 | |
| Corticosterone | 347 | 311 | 80 | 24 | |
| Corticosterone d4 | 351 | 121 | 80 | 31 |
Fig. 1
The concentrations of cortisol (A) and cortisone (B) in oral fluid of swine. In the box plots, the boundary of the box closest to zero indicates the 25th percentile, the line within the box marks the median, and the boundary of the box farthest from zero indicates the 75th percentile. The whiskers below and above the box indicate the minimum and maximum values, respectively. Results that differ statistically from the control are marked with an asterisk (*)
Fig. 2
The concentration of cortisol (A) and cortisone (B) in swine plasma collected at 8 a.m. The results of evaluation of samples from individual animals are presented, together with the mean of all results for the stressed and control groups. Statistically significant difference between the control and treated groups is marked with an asterisk (*). The results are presented for the five pigs which were sampled every other day throughout the experiment. Two other animals were excluded from blood sampling on some days due to extensive swelling of the injection site
Fig. 3
Alpha-amylase activity in swine oral fluid expressed in milliunits (mU). Statistically significant difference between the control and experimental group is marked with an asterisk (*)
Fig. 4
Total antioxidant capacity (TAC) in swine oral fluid, based on the trolox equivalent