Have a personal or library account? Click to login
The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice Cover

The protective action of piperlongumine against mycobacterial pulmonary tuberculosis in its mitigation of inflammation and macrophage infiltration in male BALB/c mice

Journal of Veterinary Research
Volume 65 (2021): Issue 4 (December 2021)
By:
Open Access
|Nov 2021

Figures & Tables

Fig. 1

Effect of piperlongumine (PL) on cell viability in MH-S (6 × 105) cells cultured for 48 h with and without PL (2.5–45 μg/mL). Dimethyl sulphoxide (DMSO) was used as internal reference for comparison. Data are expressed as mean ± SD and show no significant differences between DMSO and PL treated cells
Effect of piperlongumine (PL) on cell viability in MH-S (6 × 105) cells cultured for 48 h with and without PL (2.5–45 μg/mL). Dimethyl sulphoxide (DMSO) was used as internal reference for comparison. Data are expressed as mean ± SD and show no significant differences between DMSO and PL treated cells

Fig. 2a

In vitro effects of piperlongumine (PL) on the inhibition production of TNF-α and IL-6 in trehalose 6,6-dimycolate (TDM)-activated MH-S cells. Data are expressed as mean ± SD of three experiments. DMSO – dimethyl sulphoxide; P1 –PL at 5 μg/mL; P2 – PL at 15 μg/mL; P3 – PL at 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
In vitro effects of piperlongumine (PL) on the inhibition production of TNF-α and IL-6 in trehalose 6,6-dimycolate (TDM)-activated MH-S cells. Data are expressed as mean ± SD of three experiments. DMSO – dimethyl sulphoxide; P1 –PL at 5 μg/mL; P2 – PL at 15 μg/mL; P3 – PL at 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group

Fig. 2b

In vitro effects of piperlongumine (PL) on the inhibition of production of C-C motif chemokine ligand 2 (CCL-2), C-C motif chemokine ligand 10 (CXCL-10), C-C motif chemokine ligand 5 (CCL-5) and keratinocyte-derived chemokine (KC) in trehalose 6,6-dimycolate (TDM)-activated MH-S cells. Data are expressed as mean ± SD of three experiments. DMSO – dimethyl sulphoxide; P1 – PL 5 μg/mL; P2 – PL 15 μg/mL; P3 – PL 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
In vitro effects of piperlongumine (PL) on the inhibition of production of C-C motif chemokine ligand 2 (CCL-2), C-C motif chemokine ligand 10 (CXCL-10), C-C motif chemokine ligand 5 (CCL-5) and keratinocyte-derived chemokine (KC) in trehalose 6,6-dimycolate (TDM)-activated MH-S cells. Data are expressed as mean ± SD of three experiments. DMSO – dimethyl sulphoxide; P1 – PL 5 μg/mL; P2 – PL 15 μg/mL; P3 – PL 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group

Fig. 3

Enhancing effect of piperlongumine (PL) on the clearance of mycobacterium from alveolar macrophages. Data are expressed as mean ± SD of three experiments. TDM – trehalose-6,6-dimycolate; DMSO – dimethyl sulphoxide; P1 – PL at 5 μg/mL; P2 – PL at 15 μg/mL; P3 – PL at 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; ** P < 0.01; *** P < 0.001 vs M. tuberculosis H37Rv strain infection group
Enhancing effect of piperlongumine (PL) on the clearance of mycobacterium from alveolar macrophages. Data are expressed as mean ± SD of three experiments. TDM – trehalose-6,6-dimycolate; DMSO – dimethyl sulphoxide; P1 – PL at 5 μg/mL; P2 – PL at 15 μg/mL; P3 – PL at 25 μg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; ** P < 0.01; *** P < 0.001 vs M. tuberculosis H37Rv strain infection group

Fig. 5

Diminution by piperlongumine of trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. A) Haematoxylin and eosin-stained granulomatous response in BALB/c mice after 4–7 days of piperlongumine (PL) treatment after TDM challenge. B) Inflammatory intensity level in lungs removed from TDM-challenged mice on days 4 and 7. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 – PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P <0.001 vs TDM-treated group
Diminution by piperlongumine of trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. A) Haematoxylin and eosin-stained granulomatous response in BALB/c mice after 4–7 days of piperlongumine (PL) treatment after TDM challenge. B) Inflammatory intensity level in lungs removed from TDM-challenged mice on days 4 and 7. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 – PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P <0.001 vs TDM-treated group

Fig. 6

Effect of piperlongumine on trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. Graphs show production of tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), keratinocyte-derived cytokine (KC), C-C motif chemokine ligand 5 (CCL-5), C-C motif chemokine ligand 10 (CXCL-10) and C-C motif chemokine ligand 2 (CCL-2) in pulmonary homogenates after 4 and 7 days of TDM stimulation with or without piperlongumine (PL) treatment. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 – PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
Effect of piperlongumine on trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. Graphs show production of tumour necrosis factor alpha (TNF-α), interleukin 6 (IL-6), interleukin 10 (IL-10), keratinocyte-derived cytokine (KC), C-C motif chemokine ligand 5 (CCL-5), C-C motif chemokine ligand 10 (CXCL-10) and C-C motif chemokine ligand 2 (CCL-2) in pulmonary homogenates after 4 and 7 days of TDM stimulation with or without piperlongumine (PL) treatment. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 – PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group

Fig. 7

Inhibition by piperlongumine (PL) of leukocyte infiltration in trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. A) CD11b+ Ly6G−macrophages. B) CD11b+ lymphocyte antigen 6 complex locus G6D (Ly6G+)–neutrophils. C) chemokine receptor type 3 (CCR3)+ CD16/32−eosinophils. Bar charts represent quantitative measurement of positively stained cells. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 –PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
Inhibition by piperlongumine (PL) of leukocyte infiltration in trehalose 6,6-dimycolate (TDM)-stimulated pulmonary granulomatous inflammation in BALB/c mice. Data are expressed as mean ± SD of three experiments. A) CD11b+ Ly6G−macrophages. B) CD11b+ lymphocyte antigen 6 complex locus G6D (Ly6G+)–neutrophils. C) chemokine receptor type 3 (CCR3)+ CD16/32−eosinophils. Bar charts represent quantitative measurement of positively stained cells. P1 – PL at 50 mg/mL; P2 – PL at 100 mg/mL; P3 –PL at 150 mg/mL; DEX – dexamethasone; ns – non-significant, P > 0.05; * P < 0.05; ** P < 0.01; *** P < 0.001 vs TDM-treated group
DOI: https://doi.org/10.2478/jvetres-2021-0061 | Journal eISSN: 2450-8608
Journal RSS Feed
Language: English
Page range: 431 - 440
Submitted on: Apr 2, 2021
Accepted on: Oct 26, 2021
Published on: Nov 20, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 times per year
Keywords:
tuberculosis,
piperlongumine,
macrophages,
inflammation,
Mincle
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Nihong Lu, Yongrui Yang, Xiaofei Li, Jie Li, Jie Cheng, Zhengxuan Lv, Yingrong Du, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

Previous articleVolume 65 (2021): Issue 4 (December 2021)Next article