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Protective effects of bisoprolol against cadmium-induced myocardial toxicity through inhibition of oxidative stress and NF-κΒ signalling in rats Cover

Protective effects of bisoprolol against cadmium-induced myocardial toxicity through inhibition of oxidative stress and NF-κΒ signalling in rats

Journal of Veterinary Research
Volume 65 (2021): Issue 4 (December 2021)
By: Jinhua Liu,  Ying Xie,  Zhujun Han,  Hailong Wang and  Wenhu Xu  
Open Access
|Oct 2021

Figures & Tables

Fig. 1a

Mean arterial pressure (MAP) in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Mean arterial pressure (MAP) in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 1b

Serum creatine kinase-MB (CK-MB) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Serum creatine kinase-MB (CK-MB) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 1c

Serum lactic acid dehydrogenase (LDH) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Serum lactic acid dehydrogenase (LDH) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 1d

Serum alanine aminotransferase (ALT) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Serum alanine aminotransferase (ALT) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 1e

Serum aspartate aminotransferase (AST) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Serum aspartate aminotransferase (AST) levels in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 2

Malondialdehyde (MDA) levels in cardiac tissue from rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Malondialdehyde (MDA) levels in cardiac tissue from rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 3

Tumour necrosis factor alpha (TNF-α) from cardiac tissue of rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Tumour necrosis factor alpha (TNF-α) from cardiac tissue of rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significant difference versus the control group; ** P < 0.001 for significant difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 4a

Cardiac tissue homogenate NF-κB- p65/β-actin expression from Western blot analysis in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significance difference versus the control group; ** P < 0.001 for significance difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Cardiac tissue homogenate NF-κB- p65/β-actin expression from Western blot analysis in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significance difference versus the control group; ** P < 0.001 for significance difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 4b

Cardiac tissue homogenate GST/β-actin expression from Western blot analysis in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significance difference versus the control group; ** P < 0.001 for significance difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)
Cardiac tissue homogenate GST/β-actin expression from Western blot analysis in rats treated with cadmium and bisoprolol (BIS) (2 and 8 mg/kg/day). Values are expressed as mean ± SEM (n = 9). *** P < 0.0001 for significance difference versus the control group; ** P < 0.001 for significance difference versus the Cd-treated group (using one-way ANOVA, including Dunnett’s test)

Fig. 5

Effect of bisoprolol on cadmium-induced cardiotoxicity. I – the control group showing normally preserved cardiac muscle architecture; II – the cadmium-treated group showing subendocardial necrosis with no muscle striations, pyknotic nuclei and vascular congestion; III – the cadmium and 2 mg/kg BIS-treated group showing moderate improvement with congestion and no muscle striations; IV – the cadmium and 8 mg/kg BIS-treated group showing marked improvement with mild congestion
Effect of bisoprolol on cadmium-induced cardiotoxicity. I – the control group showing normally preserved cardiac muscle architecture; II – the cadmium-treated group showing subendocardial necrosis with no muscle striations, pyknotic nuclei and vascular congestion; III – the cadmium and 2 mg/kg BIS-treated group showing moderate improvement with congestion and no muscle striations; IV – the cadmium and 8 mg/kg BIS-treated group showing marked improvement with mild congestion

Quantitative data on the cardiac tissue antioxidant enzymes in rats treated with cadmium and BIS (2 and 8 mg/kg/day)

ParameterControlCadmiumBIS (2 mg/kg)BIS (8 mg/kg)
SOD (unit of SOD/g tissue)41.5 ± 3.6719.81 ± 0.67 ***21.1 ± 3.12 #36.76 ± 1.89 **
CAT (mmol/min/g tissue)3.32 ± 1.642.897 ± 0.34 ***2.521 ± 0.22 #3.167 ± 0.289 **
LPO (nmol hydroperoxides/g tissue)894.3 ± 14.611639 ± 56.23 ***1556.5 ± 45.23#823.8 ± 18.13**
DOI: https://doi.org/10.2478/jvetres-2021-0054 | Journal eISSN: 2450-8608
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Language: English
Page range: 505 - 511
Submitted on: Mar 27, 2021
Accepted on: Sep 30, 2021
Published on: Oct 20, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
cadmium,
bisoprolol,
catalase,
myocardial toxicity
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Jinhua Liu, Ying Xie, Zhujun Han, Hailong Wang, Wenhu Xu, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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