Table 1
Genes and sequences of primers used in the experiment
| Gene name | GenBank number | 5ʹ→3ʹ | Exon junction | Product length (bp) | |
|---|---|---|---|---|---|
| Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) | NM_017008.4 | Forward | CATGGCCTTCCGTGTTCCTA | 74 | |
| Reverse | ACTTGGCAGGTTTCTCCAGG | 825/826 | |||
| Nuclear factor of kappa-light-chain- enhancer in B - cells 1 (NF-κB1) | NM_001276711.1 | Forward | GCCAACTGGCAGGTATTTGAC | 2694/2695 | 117 |
| Reverse | TTGCAGCCTCGTGTCTTCTG | ||||
| Nuclear factor of kappa-light-chain- enhancer in B - cells 2, p49/p100 (NF-κB2) | NM_001008349.1 | Forward | TGGTACAGAGCGGTAAGAGTG | 2326/2327 | 134 |
| Reverse | TCTGTCTCAGCCAGGCTACC | ||||
| Interleukin 10 (IL-10) | NM_012854.2 | Forward | TGCGACGCTGTCATCGATTT | 379/380 | 129 |
| Reverse | TGGCCTTGTAGACACCTTTGT | ||||
| Tumour necrosis factor alpha (TNF-α) | NM_012675.3 | Forward | ATGGGCTCCCTCTCATCAGT | 106 | |
| Reverse | GCTTGGTGGTTTGCTACGAC | 433/434 | |||
| Interleukin 1beta (IL-1β) | NM_031512.2 | Forward | CCTATGTCTTGCCCGTGGAG | 82 | |
| Reverse | AGAGGACGGGCTCTTCTTCAA | 354/355 | |||
| Transforming growth factor, beta 1 (TGF-β1) | NM_021578.2 | Forward | CTGCTGACCCCCACTGATAC | 94 | |
| Reverse | AGCCCTGTATTCCGTCTCCT | 779/780 | |||
Fig. 1
Changes in interleukin expression in the lungs. Ctrl – Group I (control); 3 h – Group II; 24 h – Group III; 48 h – Group IV. Significance levels are indicated with asterisks: * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001. Ns – not statistically significant
Fig. 2
Changes in interleukin expression in the liver. Ctrl – Group I (control); 3h – Group II; 24h – Group III; 48h – Group IV. Significance levels are indicated with asterisks: * P ≤ 0.05; ** P ≤ 0.01; *** P ≤ 0.001. Ns – not statistically significant