Fig. 1
Geographical origin of Rhipicephalus bursa (total collected and Ehrlichia-positive) in south-eastern Romania
Table 1
Primers used for the detection of Rickettsiales DNA in Rhipicephalus bursa
| Pathogen | Primer sequence | Target gene | Target fragment length | Reference |
|---|---|---|---|---|
| EHR16SD: GGTACCYACAGAAGAAGTCC | ||||
| Anaplasmataceae | EHR16SR: TAGCACTCATCGTTTACAGC | 16S rRNA | 345bp | 19 |
| Ge3a: CACATGCAAGTCGAACGGATTATTC | 932bp | |||
| Anaplasma spp./ | Ge10: TTCCGTTAAGAAGGATCTAATCTCC | 16S rRNA | 14 | |
| A. phagocytophilum | Ge2: AACGGATTATTCTTTATAGCTTGCT | 546bp | ||
| Ge9: GGCAGTATTAAAAGCAGCTCCAGG | ||||
| ECC: AGAACGAACGCTGGCGGCAAGCC | 500bp | |||
| Ehrlichia spp./ | ECB: CGTATTACCGCGGCTGCTGGCA | |||
| E. canis | Canis: CAATTATTTATAGCCTCTGGCTATAGGA | 16S rRNA | 389bp | 27 |
| HE3: TATAGGTACCGTCATTATCTTCCCTAT | ||||
| A. marginale/ | Msp43: CCGGATCCTTAGCTGAACAGGAATCTTGC | |||
| msp4 | 842bp | 7 | ||
| A. centrale/A. ovis | Msp45: GGGAGCTCCTATGAATTACAGAGAATTGTTTAC | |||
| EPLAT5: TTTGTCGTAGCTTGCTATGAT | ||||
| A. platys | EPLAT3: CTTCTGTGGGTACCGTC | 16S rRNA | 359bp | 18 |
| Rsfg877: GGGGGCCTGCT- CACGGCGG | ||||
| SFG Rickettsia spp. | Rsfg1258: ATTGCAAAAAGTACAGTGAACA | gltA | 359bp | 22 |
Fig. 2
Electrophoresis gel image showing positive results and including negative and positive controls and the 100-bp DNA ladder (Invitrogen, part of Thermo-Fisher Scientific, Waltham, MA, USA) as the last 3 lanes
Fig. 3
Phylogenetic tree of E. canis detected in R. bursa collected from sheep and goats in south-eastern Romania