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The presence of Ehrlichia canis in Rhipicephalus bursa ticks collected from ungulates in continental Eastern Europe Cover

The presence of Ehrlichia canis in Rhipicephalus bursa ticks collected from ungulates in continental Eastern Europe

Journal of Veterinary Research
Volume 65 (2021): Issue 3 (September 2021)
By: Ioana Adriana Matei,  Angela Monica Ionică,  Alexandra Corduneanu,  Cristian Domșa and  Attila D. Sándor  
Open Access
|Sep 2021

Figures & Tables

Fig. 1

Geographical origin of Rhipicephalus bursa (total collected and Ehrlichia-positive) in south-eastern Romania
Geographical origin of Rhipicephalus bursa (total collected and Ehrlichia-positive) in south-eastern Romania

Fig. 2

Electrophoresis gel image showing positive results and including negative and positive controls and the 100-bp DNA ladder (Invitrogen, part of Thermo-Fisher Scientific, Waltham, MA, USA) as the last 3 lanes
Electrophoresis gel image showing positive results and including negative and positive controls and the 100-bp DNA ladder (Invitrogen, part of Thermo-Fisher Scientific, Waltham, MA, USA) as the last 3 lanes

Fig. 3

Phylogenetic tree of E. canis detected in R. bursa collected from sheep and goats in south-eastern Romania
Phylogenetic tree of E. canis detected in R. bursa collected from sheep and goats in south-eastern Romania

Primers used for the detection of Rickettsiales DNA in Rhipicephalus bursa

PathogenPrimer sequenceTarget geneTarget fragment lengthReference
EHR16SD: GGTACCYACAGAAGAAGTCC
AnaplasmataceaeEHR16SR: TAGCACTCATCGTTTACAGC16S rRNA345bp19
Ge3a: CACATGCAAGTCGAACGGATTATTC 932bp
Anaplasma spp./Ge10: TTCCGTTAAGAAGGATCTAATCTCC16S rRNA 14
A. phagocytophilumGe2: AACGGATTATTCTTTATAGCTTGCT 546bp
Ge9: GGCAGTATTAAAAGCAGCTCCAGG
ECC: AGAACGAACGCTGGCGGCAAGCC 500bp
Ehrlichia spp./ECB: CGTATTACCGCGGCTGCTGGCA
E. canisCanis: CAATTATTTATAGCCTCTGGCTATAGGA16S rRNA389bp27
HE3: TATAGGTACCGTCATTATCTTCCCTAT
A. marginale/Msp43: CCGGATCCTTAGCTGAACAGGAATCTTGC
msp4842bp7
A. centrale/A. ovisMsp45: GGGAGCTCCTATGAATTACAGAGAATTGTTTAC
EPLAT5: TTTGTCGTAGCTTGCTATGAT
A. platysEPLAT3: CTTCTGTGGGTACCGTC16S rRNA359bp18
Rsfg877: GGGGGCCTGCT- CACGGCGG
SFG Rickettsia spp.Rsfg1258: ATTGCAAAAAGTACAGTGAACAgltA359bp22
DOI: https://doi.org/10.2478/jvetres-2021-0044 | Journal eISSN: 2450-8608
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Language: English
Page range: 271 - 275
Submitted on: Feb 17, 2021
Accepted on: Aug 2, 2021
Published on: Sep 23, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
spotted fever group,
Ixodidae,
livestock,
tick-borne diseases
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Ioana Adriana Matei, Angela Monica Ionică, Alexandra Corduneanu, Cristian Domșa, Attila D. Sándor, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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