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Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system Cover

Semi-stable production of bovine IL-4 and GM-CSF in the mammalian episomal expression system

Journal of Veterinary Research
Volume 65 (2021): Issue 3 (September 2021)
By: Federico Carlos Blanco,  Cristina Lourdes Vazquez,  Julia Sabio y García,  Rosana Valeria Rocha,  María José Gravisaco,  Marina Andrea Forrellad,  Giovanni Magistrelli and  Fabiana Bigi  
Open Access
|Aug 2021

Figures & Tables

Fig. 1

PEAKrapid CRL-2828 cells transfected with plasmids expressing bovine IL-4 and bovine GM-CSF together with plasmids expressing GFP
PEAKrapid CRL-2828 cells transfected with plasmids expressing bovine IL-4 and bovine GM-CSF together with plasmids expressing GFP

Fig. 2

Expression of rIL-4 and rGM-CSF in culture supernatants of transfected cells. Western blot analysis of total secreted proteins from transfected cells. Total proteins were precipitated with 10 % TCA from culture supernatant of non-transfected cells (1) of cells transfected with plasmid expressing IL-4 (2 and 4) or GM-CSF (3and 5). Molecular weight marker was loaded on lane 6
Expression of rIL-4 and rGM-CSF in culture supernatants of transfected cells. Western blot analysis of total secreted proteins from transfected cells. Total proteins were precipitated with 10 % TCA from culture supernatant of non-transfected cells (1) of cells transfected with plasmid expressing IL-4 (2 and 4) or GM-CSF (3and 5). Molecular weight marker was loaded on lane 6

Fig. 3

Functional activity of culture supernatant of IL-4 and GM-CSF transfected cells. Bovine monocytes were incubated with 10% v/v cell culture supernatant containing rIL-4 and/or rGM-CSF. As a control, monocytes were incubated with 10% v/v cell culture supernatant of non-transfected cells. Cell morphologies were registered with a confocal microscope
Functional activity of culture supernatant of IL-4 and GM-CSF transfected cells. Bovine monocytes were incubated with 10% v/v cell culture supernatant containing rIL-4 and/or rGM-CSF. As a control, monocytes were incubated with 10% v/v cell culture supernatant of non-transfected cells. Cell morphologies were registered with a confocal microscope
DOI: https://doi.org/10.2478/jvetres-2021-0043 | Journal eISSN: 2450-8608
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Language: English
Page range: 315 - 321
Submitted on: Jan 22, 2021
Accepted on: Jul 26, 2021
Published on: Aug 19, 2021
Published by: National Veterinary Research Institute in Pulawy
In partnership with: Paradigm Publishing Services
Publication frequency: 4 issues per year
Keywords:
bovine GM-CSF,
bovine IL-4,
dendritic cells,
macrophages,
mammalian episomal expression
Related subjects:
Life sciences,
Molecular biology,
Microbiology and virology,
Life sciences, other,
Medicine,
Veterinary medicine

© 2021 Federico Carlos Blanco, Cristina Lourdes Vazquez, Julia Sabio y García, Rosana Valeria Rocha, María José Gravisaco, Marina Andrea Forrellad, Giovanni Magistrelli, Fabiana Bigi, published by National Veterinary Research Institute in Pulawy
This work is licensed under the Creative Commons Attribution-NonCommercial-NoDerivatives 3.0 License.

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